Chondrocyte colonisation of a tissue-engineered cartilage substitute under a mechanical stimulus

Nachtsheim, Julia; Dursun, Gözde; Markert, Bernd; Stoffel, Marcus

doi:10.1016/j.medengphy.2019.09.022

Cited by 11 publications

(12 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In line with our results of mechanical stimulation helping to recruit cells, Nachtsheim et al, found colonization of chondrocytes in an upper located col-I scaffold when using unconfined compression [29]. Nevertheless, in our system the scaffolds were stressed in a confined situation as we placed a circular compressible ring around the scaffold [18].…”

Section: Mechanical Stimulation and Mobilized Cellssupporting

confidence: 83%

“…Nachtsheim et al reported a colonization of cell-free col-I scaffolds by chondrocytes originally provided in a layer located beneath the cell free scaffold [29]. However, our preliminary results suggested that mechanical stimulation mobilizes MSCs only when working together with functionalized scaffolds [18].…”

Section: Biomechanics In the Knee Joint And The Bioreactormentioning

confidence: 56%

“…Thus, the cartilage surrounding the defect was simulated by an elastic ring made of silicone that recreated a laterally confined environment for the scaffold in our in vitro system [18]. Nachtsheim et al detected fewer chondrocytes (approximately 150 viable cells/mm 3 , 90% cell viability) in the scaffolds after 20 days of stimulation [29] than we did after 24 h for col-I scaffolds (245 cells/mm 3 , 93.5% viability), probably because long runs may imply a higher risk of losing cells by apoptosis and necrosis. In the present study, the viability of the moved cells was slightly higher under dynamic stimulation than the viability of the controls without mechanical stimulation, which is comparable with the results of Nachtsheim et al [29].…”

Section: Mechanical Stimulation and Mobilized Cellsmentioning

confidence: 99%

See 2 more Smart Citations

Compression Bioreactor-Based Mechanical Loading Induces Mobilization of Human Bone Marrow-Derived Mesenchymal Stromal Cells into Collagen Scaffolds In Vitro

Gamez

Schneider-Wald

Schuette

et al. 2020

IJMS

View full text Add to dashboard Cite

Articular cartilage (AC) is an avascular tissue composed of scattered chondrocytes embedded in a dense extracellular matrix, in which nourishment takes place via the synovial fluid at the surface. AC has a limited intrinsic healing capacity, and thus mainly surgical techniques have been used to relieve pain and improve function. Approaches to promote regeneration remain challenging. The microfracture (MF) approach targets the bone marrow (BM) as a source of factors and progenitor cells to heal chondral defects in situ by opening small holes in the subchondral bone. However, the original function of AC is not obtained yet. We hypothesize that mechanical stimulation can mobilize mesenchymal stromal cells (MSCs) from BM reservoirs upon MF of the subchondral bone. Thus, the aim of this study was to compare the counts of mobilized human BM-MSCs (hBM-MSCs) in alginate-laminin (alginate-Ln) or collagen-I (col-I) scaffolds upon intermittent mechanical loading. The mechanical set up within an established bioreactor consisted of 10% strain, 0.3 Hz, breaks of 10 s every 180 cycles for 24 h. Contrary to previous findings using porcine MSCs, no significant cell count was found for hBM-MSCs into alginate-Ln scaffolds upon mechanical stimulation (8 ± 5 viable cells/mm3 for loaded and 4 ± 2 viable cells/mm3 for unloaded alginate-Ln scaffolds). However, intermittent mechanical stimulation induced the mobilization of hBM-MSCs into col-I scaffolds 10-fold compared to the unloaded col-I controls (245 ± 42 viable cells/mm3 vs. 22 ± 6 viable cells/mm3, respectively; p-value < 0.0001). Cells that mobilized into the scaffolds by mechanical loading did not show morphological changes. This study confirmed that hBM-MSCs can be mobilized in vitro from a reservoir toward col-I but not alginate-Ln scaffolds upon intermittent mechanical loading, against gravity.

show abstract

Section: Mechanical Stimulation and Mobilized Cellssupporting

confidence: 83%

Section: Biomechanics In the Knee Joint And The Bioreactormentioning

confidence: 56%

Section: Mechanical Stimulation and Mobilized Cellsmentioning

confidence: 99%

See 1 more Smart Citation

Compression Bioreactor-Based Mechanical Loading Induces Mobilization of Human Bone Marrow-Derived Mesenchymal Stromal Cells into Collagen Scaffolds In Vitro

Gamez

Schneider-Wald

Schuette

et al. 2020

IJMS

View full text Add to dashboard Cite

show abstract

“…To our knowledge, this is the first study suggesting mobilization of MSCs from a low compartment to another at the top against gravity, induced by biomechanical stimulation in vitro. Migration of chondrocytes under mechanical stimulation has been previously stated [40][41][42], and migration of MSCs under mechanical stimulation was addressed in the past by Ode and collaborators, demonstrating that loading hampered the mobilization of MSCs in bone healing context [43] using a bioreactor system previously described [44]. Both bioreactors were able to apply load on a scaffold.…”

Section: Discussionmentioning

confidence: 91%

Bioreactor for mobilization of mesenchymal stem/stromal cells into scaffolds under mechanical stimulation: Preliminary results

et al. 2020

Self Cite

View full text Add to dashboard Cite

OPEN ACCESS Citation: Gamez C, Schneider-Wald B, Schuette A, Mack M, Hauk L, Khan AuM, et al. (2020) Bioreactor for mobilization of mesenchymal stem/ stromal cells into scaffolds under mechanical stimulation: Preliminary results. PLoS ONE 15(1): e0227553. https://doi.org/10.1371/journal. ResultsThe bioreactor was able to stimulate the scaffolds and the cells for 24.4 (±1.7) hours, exerting compression with vertical displacements of 185.8 (±17.8) μm and a force-amplitude of 1.87 (±1.37; min 0.31, max 4.42) N. Our results suggest that continuous mechanical stimulation hampered the viability of the cells located at the cell reservoir when comparing to intermittent mechanical stimulation (34.4 ± 2.0% vs. 66.8 ± 5.9%, respectively).Functionalizing alginate scaffolds with laminin-521 (LN521) seemed to enhance the mobilization of cells from 48 (±21) to 194 (±39) cells/mm 3 after applying intermittent mechanical loading. ConclusionThe bioreactor presented here was able to provide mechanical stimulation that seemed to induce the mobilization of MSCs into LN521-alginate scaffolds under an intermittent loading regime.Bioreactor for mobilization of MSCs under mechanical stimulation PLOS ONE | https://doi.

show abstract

“…AC is a dense connective tissue mainly comprised of collagen, proteoglycans, organized in special zones containing special types of cells called articular chondrocytes [1,2]. The biological and mechanical properties of AC are highly complex and vary zonally [3]. AC provides the joint with different biomechanical functions, e.g., wear resistance or shock absorption up to eight times of the bodyweight [4,5].…”

Section: Introductionmentioning

confidence: 99%

The Biomechanics of Cartilage—An Overview

Eschweiler

Horn

Rath

et al. 2021

Life

View full text Add to dashboard Cite

Articular cartilage (AC) sheathes joint surfaces and minimizes friction in diarthrosis. The resident cell population, chondrocytes, are surrounded by an extracellular matrix and a multitude of proteins, which bestow their unique characteristics. AC is characterized by a zonal composition (superficial (tangential) zone, middle (transitional) zone, deep zone, calcified zone) with different mechanical properties. An overview is given about different testing (load tests) methods as well as different modeling approaches. The widely accepted biomechanical test methods, e.g., the indentation analysis, are summarized and discussed. A description of the biphasic theory is also shown. This is required to understand how interstitial water contributes toward the viscoelastic behavior of AC. Furthermore, a short introduction to a more complex model is given.

show abstract

Chondrocyte colonisation of a tissue-engineered cartilage substitute under a mechanical stimulus

Cited by 11 publications

References 31 publications

Compression Bioreactor-Based Mechanical Loading Induces Mobilization of Human Bone Marrow-Derived Mesenchymal Stromal Cells into Collagen Scaffolds In Vitro

Compression Bioreactor-Based Mechanical Loading Induces Mobilization of Human Bone Marrow-Derived Mesenchymal Stromal Cells into Collagen Scaffolds In Vitro

Bioreactor for mobilization of mesenchymal stem/stromal cells into scaffolds under mechanical stimulation: Preliminary results

The Biomechanics of Cartilage—An Overview

Contact Info

Product

Resources

About