Chondrocyte Cultivation in Hyaluronan-Tyramine Cross-Linked Hydrogel

Abstract

The application of chondrocyte-seeded hydrogels, such as tyramine-substituted hyaluronan, is a highly promising strategy for damaged cartilage treatments. The rheological characteristics of hyaluronan-tyramine derivative scaffold were determined together with the hydrogel swelling, the water uptake, and the morphological characterization. The chondrocytes seeded in hyaluronantyramine hydrogel were viable and expressed markers typical for differentiated chondrocytes with no signs of hypertrophy or extensive expression of matrix metalloproteinases. The lyophilized unseeded hyaluronan-tyramine scaffolds were also implantated into trochlea osteochondral defects in rabbits. In conclusion, the hyaluronan-tyramine derivative was proven to be advisable material as a scaffold for cartilage reconstitution.

Injectable hydroxyphenyl derivative of hyaluronic acid hydrogel modified with RGD as scaffold for spinal cord injury repair

“…29,30 The cross-linking reaction of this HA-PH derivative can be triggered by enzyme horseradish peroxidase (HRP) and hydrogen peroxide (H 2 O 2 ) under physiological conditions, without a negative effect on encapsulated cells during cross-linking reaction and gel forming. 31 Previously, we demonstrated the chondrogenic potential of HA-PH derivative based hydrogel in vitro as well as in vivo in cartilage defects. 31,32 In this study, we have used the HA-PH derivative bearing the newly developed 3-(4-hydroxyphenyl) propionic acid (PH)-L-lysine-aminohexanoic acid-L-glycine-L-arginine-L-glycine-L-aspartic acid (HA-PH-RGD) sequence [ Fig.…”

“…31 Previously, we demonstrated the chondrogenic potential of HA-PH derivative based hydrogel in vitro as well as in vivo in cartilage defects. 31,32 In this study, we have used the HA-PH derivative bearing the newly developed 3-(4-hydroxyphenyl) propionic acid (PH)-L-lysine-aminohexanoic acid-L-glycine-L-arginine-L-glycine-L-aspartic acid (HA-PH-RGD) sequence [ Fig. 1(A)], which allows the attachment of PH moiety and RGD cell adhesive motive to polymer backbone in one synthetic step.…”

“…One of such promising HA‐based materials capable to form covalently cross‐linked hydrogel with the required mechanical properties, is the hydroxyphenyl derivative of HA (HA‐PH) . The cross‐linking reaction of this HA‐PH derivative can be triggered by enzyme horseradish peroxidase (HRP) and hydrogen peroxide (H 2 O 2 ) under physiological conditions, without a negative effect on encapsulated cells during cross‐linking reaction and gel forming . Previously, we demonstrated the chondrogenic potential of HA‐PH derivative based hydrogel in vitro as well as in vivo in cartilage defects …”