Chondrocyte Generation of Cartilage‐Like Tissue Following Photoencapsulation in Methacrylated Polysaccharide Solution Blends

Hayami, James W. S.; Waldman, Stephen D.; Amsden, Brian G.

doi:10.1002/mabi.201500465

Cited by 17 publications

(20 citation statements)

References 59 publications

(103 reference statements)

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“…The ability to dissect out the individual contributions of specific microenvironmental cues could have powerful consequences for elucidating the complex array of mechanisms and cellular decisionmaking processes that drive these pathological outcomes. This report also describes VIC culture in a CS-based environment, which is uncommon, despite methacrylation of CS and HA being equally practical (62,63). Thus, this study points to the crucial role that CS plays in the progression of early CAVD and highlights the importance of expanding the examination of GAGs in the aortic valve beyond HA.…”

Section: Discussionmentioning

confidence: 74%

Creation of disease-inspired biomaterial environments to mimic pathological events in early calcific aortic valve disease

Porras

Westlund

Evans

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

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An insufficient understanding of calcific aortic valve disease (CAVD) pathogenesis remains a major obstacle in developing treatment strategies for this disease. The aim of the present study was to create engineered environments that mimic the earliest known features of CAVD and apply this in vitro platform to decipher relationships relevant to early valve lesion pathobiology. Glycosaminoglycan (GAG) enrichment is a dominant hallmark of early CAVD, but culture of valvular interstitial cells (VICs) in biomaterial environments containing pathological amounts of hyaluronic acid (HA) or chondroitin sulfate (CS) did not directly increase indicators of disease progression such as VIC activation or inflammatory cytokine production. However, HA-enriched matrices increased production of vascular endothelial growth factor (VEGF), while matrices displaying pathological levels of CS were effective at retaining lipoproteins, whose deposition is also found in early CAVD. Retained oxidized low-density lipoprotein (oxLDL), in turn, stimulated myofibroblastic VIC differentiation and secretion of numerous inflammatory cytokines. OxLDL also increased VIC deposition of GAGs, thereby creating a positive feedback loop to further enrich GAG content and promote disease progression. Using this disease-inspired in vitro platform, we were able to model a complex, multistep pathological sequence, with our findings suggesting distinct roles for individual GAGs in outcomes related to valve lesion progression, as well as key differences in cell-lipoprotein interactions compared with atherosclerosis. We propose a pathogenesis cascade that may be relevant to understanding early CAVD and envision the extension of such models to investigate other tissue pathologies or test pharmacological treatments.

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Section: Discussionmentioning

confidence: 74%

Creation of disease-inspired biomaterial environments to mimic pathological events in early calcific aortic valve disease

Porras

Westlund

Evans

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

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“…Cells (chondrocytes or mesenchymal stem cells) are encapsulated in MeHA hydrogels functionalized with methacrylated chondroitin sulfate, devitalized cartilage microparticles or N-cadherin mimetic peptides (Figure 4C). These encapsulated cells demonstrate increased expression of cartilage markers and deposition of collagen type II compared with those encapsulated in pristine MeHA hydrogels [98,112,113]. In addition, MeHA/GelMA hybrid hydrogel has been used to engineer 3D in vitro fibrotic heart valve tissue models for understanding the process of fibrotic remodeling and calcification in heart valve diseases [107,114,115].…”

Section: Synthesis Properties and Recent Biomedical Applications Of Phmentioning

confidence: 99%

Recent Advances in Photo-Crosslinkable Hydrogels for Biomedical Applications

et al. 2019

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Photo-crosslinkable hydrogels have recently attracted significant scientific interest. Their properties can be manipulated in a spatiotemporal manner through exposure to light to achieve the desirable functionality for various biomedical applications. This review article discusses the recent advances of the most common photo-crosslinkable hydrogels, including poly(ethylene glycol) diacrylate, gelatin methacryloyl and methacrylated hyaluronic acid, for various biomedical applications. We first highlight the advantages of photopolymerization and discuss diverse photosensitive systems used for the synthesis of photo-crosslinkable hydrogels. We then introduce their synthesis methods and review their latest state of development in biomedical applications, including tissue engineering and regenerative medicine, drug delivery, cancer therapies and biosensing. Lastly, the existing challenges and future perspectives of engineering photo-crosslinkable hydrogels for biomedical applications are briefly discussed.

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“…Natural origin hydrogels can serve as suitable materials that have been proposed in literature. Some of the commonly used hydrogels for regeneration of nucleus pulposus are based on the following raw materials: alginate [119,120], chitosan [121,122,123], collagen [124], gellan gum [125,126], and hyaluronic acid [127,128].…”

Section: Application Of Smart Hydrogels For Tissue Engineeringmentioning

confidence: 99%

Smart Hydrogels in Tissue Engineering and Regenerative Medicine

et al. 2019

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The field of regenerative medicine has tremendous potential for improved treatment outcomes and has been stimulated by advances made in bioengineering over the last few decades. The strategies of engineering tissues and assembling functional constructs that are capable of restoring, retaining, and revitalizing lost tissues and organs have impacted the whole spectrum of medicine and health care. Techniques to combine biomimetic materials, cells, and bioactive molecules play a decisive role in promoting the regeneration of damaged tissues or as therapeutic systems. Hydrogels have been used as one of the most common tissue engineering scaffolds over the past two decades due to their ability to maintain a distinct 3D structure, to provide mechanical support for the cells in the engineered tissues, and to simulate the native extracellular matrix. The high water content of hydrogels can provide an ideal environment for cell survival, and structure which mimics the native tissues. Hydrogel systems have been serving as a supportive matrix for cell immobilization and growth factor delivery. This review outlines a brief description of the properties, structure, synthesis and fabrication methods, applications, and future perspectives of smart hydrogels in tissue engineering.

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Chondrocyte Generation of Cartilage‐Like Tissue Following Photoencapsulation in Methacrylated Polysaccharide Solution Blends

Cited by 17 publications

References 59 publications

Creation of disease-inspired biomaterial environments to mimic pathological events in early calcific aortic valve disease

Creation of disease-inspired biomaterial environments to mimic pathological events in early calcific aortic valve disease

Recent Advances in Photo-Crosslinkable Hydrogels for Biomedical Applications

Smart Hydrogels in Tissue Engineering and Regenerative Medicine

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