Chondrocytes respond both anabolically and catabolically to impact loading generally considered non-injurious

Santos, Stephany; Richard, Kelsey; Fisher, Melanie C.; Dealy, Caroline N.; Pierce, David M.

doi:10.1016/j.jmbbm.2020.104252

Cited by 5 publications

(6 citation statements)

References 49 publications

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“…The articular cartilage chondrocytes are well-specialized cells that are sensitive to mechanical stimuli, which are a complex combination of compression, stretching, shear stress and hydrostatic pressure. Mechanical loading increased SOX9 IHC labeling in the superficial zone of bovine cartilage cultured as cylindrical osteochondral plugs [ 43 ]. The application of bioreactor-generated joint-like movements demonstrated the mechanosensitivity of ACPC cultures [ 44 ].…”

Section: Autologous Chondrocytes From Different Sourcesmentioning

confidence: 99%

Strategies to Convert Cells into Hyaline Cartilage: Magic Spells for Adult Stem Cells

Kurenkova

Романова

Kibirskiy

et al. 2022

IJMS

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Damaged hyaline cartilage gradually decreases joint function and growing pain significantly reduces the quality of a patient’s life. The clinically approved procedure of autologous chondrocyte implantation (ACI) for treating knee cartilage lesions has several limits, including the absence of healthy articular cartilage tissues for cell isolation and difficulties related to the chondrocyte expansion in vitro. Today, various ACI modifications are being developed using autologous chondrocytes from alternative sources, such as the auricles, nose and ribs. Adult stem cells from different tissues are also of great interest due to their less traumatic material extraction and their innate abilities of active proliferation and chondrogenic differentiation. According to the different adult stem cell types and their origin, various strategies have been proposed for stem cell expansion and initiation of their chondrogenic differentiation. The current review presents the diversity in developing applied techniques based on autologous adult stem cell differentiation to hyaline cartilage tissue and targeted to articular cartilage damage therapy.

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Section: Autologous Chondrocytes From Different Sourcesmentioning

confidence: 99%

Strategies to Convert Cells into Hyaline Cartilage: Magic Spells for Adult Stem Cells

Kurenkova

Романова

Kibirskiy

et al. 2022

IJMS

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“…Recent studies have revealed that EGFR signaling pathways are multifunctional cytokines that are closely associated with pathological and physiological processes in a variety of human organs and tissues and play an important role in regulating the growth and metabolism of chondrocytes [ 27 ]. Furthermore, EGFR signaling is critical for preventing OA initiation and maintaining the superficial layer of articular cartilage [ 12 ].…”

Section: Discussionmentioning

confidence: 99%

SIRT1 restoration enhances chondrocyte autophagy in osteoarthritis through PTEN-mediated EGFR ubiquitination

Liu

Miao

et al. 2022

Cell Death Discov.

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The pharmacological interventions aimed at activating pathways inducing chondrocyte autophagy or reversing extracellular matrix degradation may be promising approaches for the management of osteoarthritis (OA). Evidence exists suggesting that sirtuin 1 (SIRT1) is involved in the pathogenesis of OA. The present study aimed to explore the regulatory role and downstream mechanisms of SIRT1 in OA. Bioinformatics predictions identified downstream factors phosphatase and tensin homolog (PTEN) and epidermal growth factor receptor (EGFR) in OA. We validated poorly expressed SIRT1 and EGFR and highly expressed PTEN in cartilage tissues of OA patients. OA was induced in vitro by exposing human primary chondrocytes to IL-1β and in vivo by destabilization of the medial meniscus (DMM) in a mouse model. SIRT1 knockdown was found to augment IL-1β-stimulated inflammation and chondrocyte metabolic imbalance. Knockdown of SIRT1 diminished PTEN acetylation and then enhanced PTEN expression. PTEN inactivation decreased EGFR ubiquitination and promoted EGFR expression by destabilizing the EGFR-Cbl complex, which in turn inhibited extracellular matrix degradation in cartilage tissues and activated chondrocyte autophagy. In the DMM mouse model, knockdown of SIRT1 inhibited chondrocyte autophagy, promoted metabolic imbalance, thus accelerating osteoarthritic process. In conclusion, SIRT1 represses the ubiquitination of EGFR by down-regulating PTEN, inhibits extracellular matrix degradation and activates chondrocyte autophagy, thereby performing an OA-alleviating role.

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“…Microdamage to the collagen matrix and loss of mechanical integrity in cartilage highlights the potential for repair strategies aimed at mitigating the initiation and progression of microcracks within cartilage. Extended deterioration of extracellular matrix including growth of microcracks, coupled with catabolic responses from cells [ 10 ], may define some of the key early stages of OA pathogenesis [ 11 ]. Consequently we sought to investigate possible therapeutics that would slow or arrest the progression of microcracks, or even heal them, and minimize the possibility of escalating pathologies within cartilage and joints.…”

Section: Introductionmentioning

confidence: 99%

Genipin does not reduce the initiation or propagation of microcracks in collagen networks of cartilage

Santos

Neu

Grady

et al. 2022

Osteoarthritis and Cartilage Open

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Chondrocytes respond both anabolically and catabolically to impact loading generally considered non-injurious

Cited by 5 publications

References 49 publications

Strategies to Convert Cells into Hyaline Cartilage: Magic Spells for Adult Stem Cells

Strategies to Convert Cells into Hyaline Cartilage: Magic Spells for Adult Stem Cells

SIRT1 restoration enhances chondrocyte autophagy in osteoarthritis through PTEN-mediated EGFR ubiquitination

Genipin does not reduce the initiation or propagation of microcracks in collagen networks of cartilage

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