Chondrogenesis in a Cell-Polymer-Bioreactor System

Freed, Lisa E.; Hollander, Anthony P.; Martin, Ivan; Barry, Jeffrey; Langer, Robert; Vunjak-Novakovic, Gordana

doi:10.1006/excr.1998.4010

Cited by 416 publications

(291 citation statements)

References 28 publications

Supporting

Mentioning

269

Contrasting

Unclassified

Order By: Relevance

“…32 In HYAFF 1 -M, chondrogenesis was initiated at the construct periphery and progressed towards the construct center, as previously reported with calf articular chondrocytes cultured in polyglycolic acid nonwoven meshes. 33 The different pattern of tissue development in PEGT/ PBT scaffolds, where cells and matrix did not accumulate in the inner regions, were likely due to the reduced porosity and pore interconnectivity of the scaffolds. 19 Further studies should investigate the possibility of enhancing the uniformity of the generated tissues by using bioreactors that increase mass transfer rates by the application of specific regimes of fluid flow.…”

Section: Discussionmentioning

confidence: 99%

Cartilage tissue engineering by expanded goat articular chondrocytes

Miot

Freitas

Wirz

et al. 2006

Journal Orthopaedic Research

Self Cite

View full text Add to dashboard Cite

In this study we investigated whether expanded goat chondrocytes have the capacity to generate cartilaginous tissues with biochemical and biomechanical properties improving with time in culture. Goat chondrocytes were expanded in monolayer with or without combinations of FGF-2, TGF-b1, and PDGFbb, and the postexpansion chondrogenic capacity assessed in pellet cultures. Expanded chondrocytes were also cultured for up to 6 weeks in HYAFF 1 -M nonwoven meshes or Polyactive TM foams, and the resulting cartilaginous tissues were assessed histologically, biochemically, and biomechanically. Supplementation of the expansion medium with FGF-2 increased the proliferation rate of goat chondrocytes and enhanced their postexpansion chondrogenic capacity. FGF-2-expanded chondrocytes seeded in HYAFF 1 -M or Polyactive TM scaffolds formed cartilaginous tissues with wet weight, glycosaminoglycan, and collagen content, increasing from 2 days to 6 weeks culture (up to respectively 2-, 8-, and 41-fold). Equilibrium and dynamic stiffness measured in HYAFF 1 -M-based constructs also increased with time, up to, respectively, 1.3-and 16-fold. This study demonstrates the feasibility to engineer goat cartilaginous tissues at different stages of development by varying culture time, and thus opens the possibility to test the effect of maturation stage of engineered cartilage on the outcome of cartilage repair in orthotopic goat models. ß

show abstract

Section: Discussionmentioning

confidence: 99%

Cartilage tissue engineering by expanded goat articular chondrocytes

Miot

Freitas

Wirz

et al. 2006

Journal Orthopaedic Research

Self Cite

View full text Add to dashboard Cite

show abstract

“…This value for q cell was derived based on the case of an initial cell seeding density of 10 million cells cm −3 , free swelling culture in 2% agarose with medium containing 10% serum, where matrix accumulation showed a linear increase in time over 42 days, 40 the percentage wet weight GAG and collagen were pooled and a construct density of 1 g cm −3 was assumed (natural cartilage: 1.15 g cm −3 ). 16 Note that the value for q cell is an underestimate, since it was based on matrix accumulation data in the construct and did not account for matrix molecules lost to the medium.…”

Section: Methodsmentioning

confidence: 99%

“…15,41,47,51,56 In addition the thus formed extracellular matrix is also subject to physical, chemical and chondrocyte mediated degradation, leading to a constant matrix remodeling. 10,50,53 In the course of time the construct's collagen and proteoglycan content can approach a steady state, 16 resulting from a balance between synthesis, incorporation and degradation and/or product inhibited synthesis. 23,43,62 The relation between the content of extracellular matrix constituents and biomechanical function has been investigated in various studies.…”

Section: Introductionmentioning

confidence: 99%

The Local Matrix Distribution and the Functional Development of Tissue Engineered Cartilage, a Finite Element Study

et al. 2004

View full text Add to dashboard Cite

Abstract-Assessment of the functionality of tissue engineered cartilage constructs is hampered by the lack of correlation between global measurements of extra cellular matrix constituents and the global mechanical properties. Based on patterns of matrix deposition around individual cells, it has been hypothesized previously, that mechanical functionality arises when contact occurs between zones of matrix associated with individual cells. The objective of this study is to determine whether the local distribution of newly synthesized extracellular matrix components contributes to the evolution of the mechanical properties of tissue engineered cartilage constructs. A computational homogenization approach was adopted, based on the concept of a periodic representative volume element. Local transport and immobilization of newly synthesized matrix components were described. Mechanical properties were taken dependent on the local matrix concentration and subsequently the global aggregate modulus and hydraulic permeability were derived. The transport parameters were varied to assess the effect of the evolving matrix distribution during culture. The results indicate that the overall stiffness and permeability are to a large extent insensitive to differences in local matrix distribution. This emphasizes the need for caution in the visual interpretation of tissue functionality from histology and underlines the importance of complementary measurements of the matrix's intrinsic molecular organization.

show abstract

“…For example, the most commonly used cell seeding technique, gravity cell seeding, usually results in a vertical cell density gradient within the scaffold 5 . Alternative methods, such as the use of spinning flasks 6 or vacuum chamber 7 , showed improved cell seeding, and more uniform cell and matrix distribution in the construct. Homogeneous cell distribution in the construct is a crucial step for uniform tissue formation during tissue engineering.…”

Section: Introductionmentioning

confidence: 99%

Cell–Nanofiber-Based Cartilage Tissue Engineering Using Improved Cell Seeding, Growth Factor, and Bioreactor Technologies

Jiang

Tuan

2008

Tissue Engineering Part A

View full text Add to dashboard Cite

Biodegradable nanofibrous scaffolds serving as an extracellular matrix substitute have been shown to be applicable for cartilage tissue engineering. However, a key challenge in using nanofibrous scaffolds for tissue engineering is that the small pore size limits the infiltration of cells, which may result in uneven cell distribution throughout the scaffold. This study describes an effective method of chondrocyte loading into nanofibrous scaffolds, which combines cell seeding, mixing, and centrifugation to form homogeneous, packed cell-nanofiber composites (CNCs). When the effects of different growth factors are compared, CNCs cultured in medium containing a combination of insulin-like growth factor-I and transforming growth factor-β1 express the highest mRNA levels of collagen type II and aggrecan. Radiolabeling analyses confirm the effect on collagen and sulfated-glycosaminoglycans (sGAG) production. Histology reveals chondrocytes with typical morphology embedded in lacuna-like space throughout the entire structure of the CNC. Upon culturing using a rotary wall vessel bioreactor, CNCs develop into a smooth, glossy cartilage-like tissue, compared to a rough-surface tissue when maintained in a static environment. Bioreactorgrown cartilage constructs produce more total collagen and sGAG, resulting in greater gain in net tissue weight, as well as express cartilage-associated genes, including collagen types II and IX, cartilage oligomeric matrix protein, and aggrecan. In addition, dynamic culture enhances the mechanical property of the engineered cartilage. Taken together, these results indicate the applicability of nanofibrous scaffolds, combined with efficient cell loading and bioreactor technology, for cell-based cartilage tissue engineering.

show abstract

Chondrogenesis in a Cell-Polymer-Bioreactor System

Cited by 416 publications

References 28 publications

Cartilage tissue engineering by expanded goat articular chondrocytes

Cartilage tissue engineering by expanded goat articular chondrocytes

The Local Matrix Distribution and the Functional Development of Tissue Engineered Cartilage, a Finite Element Study

Cell–Nanofiber-Based Cartilage Tissue Engineering Using Improved Cell Seeding, Growth Factor, and Bioreactor Technologies

Contact Info

Product

Resources

About