Chondrogenesis of Infrapatellar Fat Pad Derived Adipose Stem Cells in 3D Printed Chitosan Scaffold

Ye, Ken; Felimban, Raed; Traianedes, Kathy; Moulton, Simon E.; Wallace, Gordon G.; Chung, Johnson; Quigley, Anita; Choong, Peter F. M.; Myers, Damian E.

doi:10.1371/journal.pone.0099410

Cited by 109 publications

(79 citation statements)

References 43 publications

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“…Similar to BMSCs, ASCs have the property to differentiate into osteoblastic, chondrocytic, adipocytic and neurogenic cells [38][39][40]. The influence of estrogen on differentiation of ASCs has been demonstrated earlier but remains controversial [41,42].…”

Section: Discussionmentioning

confidence: 99%

Estrogen Receptor α and β in Mouse: Adipose-Derived Stem Cell Proliferation, Migration, and Brown Adipogenesis In Vitro

Zhang

Schmull

et al. 2016

Cell Physiol Biochem

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Background/Aims: Adipose-derived stem cells (ASCs) belong to mesenchymal stem cells and may play a potential role as seeding cells in stem cell transplantation. To be able to exploit stem cells as therapeutic tool, their defects in some important cellular functions, such as low survival rate and cellular activity, should be considered. This is especially the case for stem cells that are intended for transplantation. Of note, stem cell responses to hormones should be considered since estrogen is known to play a critical role in stem cell behavior. However, different impacts of the estrogen receptor (ER) types α and β have not been fully determined in ASC function. In this study, we investigated effects of ERα and ERβ on ASC proliferation, migration, as well as in adipogenesis. Methods: ASCs obtained from mice were cultured with 100nM ERα or ERβ agonist PPT and DPN, respectively. The ERα and ERβ antagonist ICI 182,780 (100nM) was used as control. Results: Compared to ERβ, ERα appears more potent in improving ASC proliferation and migration. Investigation of adipogenesis revealed that ERβ played a significant role in suppressing ASC-mediated brown tissue adipogenesis which is in contrast to ERα. These results correlated with reduced mRNA expression of UCP-1, PGC-1α and PPAR-γ. Conclusions: ERα plays a more critical role in promoting ASC proliferation and migration while ERβ is more potent in suppressing ASC brown adipose tissue differentiation mediated by decreased UCP-1, PGC-1α and PPAR-γ expression.

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Section: Discussionmentioning

confidence: 99%

Estrogen Receptor α and β in Mouse: Adipose-Derived Stem Cell Proliferation, Migration, and Brown Adipogenesis In Vitro

Zhang

Schmull

et al. 2016

Cell Physiol Biochem

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“…These can broadly be divided into natural or synthetic scaffolds. Natural scaffolds include substances such as carbohydrate-based materials such as chitosan (Ye et al 2014;García Cruz et al 2012;Alves da Silva et al 2011), hyaluronate (Son et al 2013;Toh et al 2012) or even protein-based structures such as collagen (Zhang et al 2012(Zhang et al , 2013aMurphy et al 2012), fibrin (Diederichs et al 2012;Park et al 2011), gelatin (Klangjorhor et al 2012;Pruksakorn et al 2009) and chondroitin (Chen et al 2013;Park et al 2010;Varghese et al 2008). Synthetic scaffolds successfully used include polyglycolic acid, polylactic acid, poly(lactic-co-glycolic acid), polyethylene glycol and polycaprolactone (Hidalgo et al 2013;Childs et al 2013;Zhang et al 2013b;Li et al 2013).…”

Section: Scaffoldsmentioning

confidence: 99%

Stem Cell Therapy for Cartilage Defects

Pastides

Khan

2015

Translational Medicine Research

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Cartilaginous defects within the articular cartilage present a treatment problem within the orthopaedic community. In cases of established osteoarthritis affecting large joints, arthroplasty is a good, well-established and predictable option. It is though a step too far for smaller and discrete lesions. Currently, surgical options include autologous chondrocyte implantation, microfracture, osteochondral autologous transplantation and even osteochondral allograft plugs. Tissue engineering techniques may prove to be the answer to this problem. There is plenty of interest in stem cell manipulation to induce chondrogenesis. The areas of research focus on the differentiation of multipotent mesenchymal stem cells but also more recently on the use of induced pluripotent stem cells. Furthermore, augmentation of repair can be facilitated by endogenous stimulants to these cells such as growth factors, gene therapy and scaffolds to maintain an optimum microenvironment. Endogenous stimulants aside, it does appear that exogenous methods of stimulation such as ultrasound and magnetic field applications can further augment and improve the reparative process. The aim of this chapter is to define the problem faced by the medical world due to the macro-and microscopic structure of cartilage and present data and reports showing the advances made in this field. The final section focuses on the current state of play surrounding the translation of these techniques to human subjects, presenting the up-to-date studies.

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“…Ideal scaffolds should be biocompatible, with a porosity to allow for the transport of oxygen and nutrients to the cells and for easy waste removal [6]. Natural materials such as chitosan and collagen demonstrate selective cell adhesion and other physiological qualities and therefore have been used to make scaffolds for a variety of cell types [7,8]. The chitosan-collagen combination also improves the mechanical properties of the scaffold and is one of the most widely used materials for a variety of scaffolds, such as for skin and cartilage reconstruction [9].…”

Section: Introductionmentioning

confidence: 99%

Design of a Novel Method for the Spatial Distribution of Cells within a Porous Scaffold for Tissue Engineering Applications

Subramanian¹,

Bhowmick²,

Gappa-Fahlenkamp³

2017

J Tissue Sci Eng

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Tissue engineering is rapidly progressing to provide complex, three-dimensional (3D) representations of human tissues that can be used for tissue replacement and/or to study tissue systems. Tissue engineering includes the addition of cells within 3D scaffolds, along with bioactive components, sometimes within a bioreactor. A major challenge in developing many tissue-engineered models is the ability to evenly distribute cells throughout a porous scaffold, in order to achieve good cell viability and growth. In this study, we created a 3D collagen-chitosan scaffold with specific properties to aid in seeding cells within the entire volume and investigated a dynamic method to seed cells within such scaffold. Based on the requirements for cell seeding, the scaffolds were less than 500 µm thick, had pore sizes greater than 50 µm and had a porosity of 50% or greater. Fibroblasts were used as model cells for this seeding method. To seed fibroblasts within the scaffold, we varied two design parameters: concentration of the collagen seeding solution and the centrifugal force used for cell seeding. We ranked the seeding efficiency, cell proliferation and distribution in order to choose the ideal cell seeding method. Results showed that seeding with a higher concentration (2 mg/ml) of collagen seeding solution and a lower centrifugation speed (259 ×g) was the optimal seeding method, resulting in 84% increase in cell proliferation and a more uniform cell distribution throughout the scaffold. Results from this study can be applied for seeding a variety of cell populations within porous scaffolds for tissue engineering applications.

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Chondrogenesis of Infrapatellar Fat Pad Derived Adipose Stem Cells in 3D Printed Chitosan Scaffold

Cited by 109 publications

References 43 publications

Estrogen Receptor α and β in Mouse: Adipose-Derived Stem Cell Proliferation, Migration, and Brown Adipogenesis In Vitro

Estrogen Receptor α and β in Mouse: Adipose-Derived Stem Cell Proliferation, Migration, and Brown Adipogenesis In Vitro

Stem Cell Therapy for Cartilage Defects

Design of a Novel Method for the Spatial Distribution of Cells within a Porous Scaffold for Tissue Engineering Applications

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