Chondrogenic potentials of human synovium-derived cells sorted by specific surface markers

Chang, Chong Bum; Han, Sangbin; Kim, E.M.; Lee, Seungmee; Seong, Sang Cheol; Lee, M.C.

doi:10.1016/j.joca.2012.10.005

Cited by 36 publications

(23 citation statements)

References 33 publications

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“…The ADSCs of murine origin expressed CD105 but its expression varied with passage number and cell density. When ADSCs were CD105 negative, they were more inclined to differentiate into osteocytes and adipocytes [Anderson et al, 2013], whereas the presence of CD105 favored chondrogenic transformation [Chang et al, 2013]. In our study, the decrease in CD105 mRNA abundance in the absence of FBS (TRT2 and TRT4) is, therefore, indicative that the absence of FBS promotes lineage differentiation towards adipocytes irrespective of the fact that CD105 was still expressed at a low level in each TRT.…”

Section: Discussionmentioning

confidence: 45%

Influence of Bovine Serum Lipids and Fetal Bovine Serum on the Expression of Cell Surface Markers in Cultured Bovine Preadipocytes

Sandhu¹,

Jurek²,

Trappe³

et al. 2017

Cells Tissues Organs

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To establish the influence of fetal bovine serum (FBS) and bovine serum lipids (BSL) on cell differentiation marker expression, bovine adipose-derived stem cells from subcutaneous tissue were incubated for 14 days in 4 types of differentiation media containing 10% FBS and 10 µL/mL BSL (TRT-1), no FBS and 10 µL/mL of BSL (TRT-2), 10% FBS and no BSL (TRT-3), or no supplements (TRT-4). Cells were subjected to Nile red staining, immunocytochemistry (CD73, CD90, CD105, DLK1, FabP4), and quantitative real-time PCR (CD73, CD90, CD105, FabP4). The number of cells presenting FabP4 and the percentage of mature adipocytes with large lipid droplets were increased in TRT-2, accompanied by a robust increase in FabP4 mRNA abundance and a decrease in DLK1-positive cells. In preadipocytes, CD73 was present around the nucleus and translocated towards cell membranes during differentiation. Although the percentage of CD73-positive cells was not different among treatments, its mRNA abundance, immunocytochemical staining intensity, and translocation towards cell membranes were decreased when the medium contained no FBS (TRT-2 and TRT-4). All cells showed a diffuse distribution of CD90 and CD105 and remained positive for these markers irrespective of the treatment. However, the CD90 and CD105 mRNA abundance was decreased in TRT-2 and TRT-4; i.e., in media containing no FBS. The presence of FBS increased the absolute number of cell nuclei as assessed by DAPI fluorescence. Our results suggest that bovine subcutaneous preadipocytes display typical stem cell markers. The differentiation into mature adipocytes is promoted by BSL, whereas FBS endorses cell proliferation.

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Section: Discussionmentioning

confidence: 45%

Influence of Bovine Serum Lipids and Fetal Bovine Serum on the Expression of Cell Surface Markers in Cultured Bovine Preadipocytes

Sandhu¹,

Jurek²,

Trappe³

et al. 2017

Cells Tissues Organs

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“…Accordingly, CD73 is highly expressed on synovial stromal cells (10). A second possibility is that CD73 expression on endothelial cells regulates joint inflammation.…”

Section: Discussionmentioning

confidence: 99%

“…CD73 on nonhematopoietic cells plays an important role in the control of CIA CD73 is expressed on both hematopoietic and nonhematopoietic cells (10). Notably, CD73 is expressed at high levels on human joint synovial cells (Supplemental Fig.…”

Section: Cd73-deficient Mice Are More Susceptible To Ciamentioning

confidence: 99%

CD73 Plays a Protective Role in Collagen-Induced Arthritis

Chrobák

Charlebois

Rejtar

et al. 2015

The Journal of Immunology

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Rheumatoid arthritis (RA) is a chronic autoimmune disease with significant morbidity and mortality. Recent studies suggest that modulation of adenosine signaling, a potent immunosuppressive pathway, is a promising approach for treatment of RA. Extracellular adenosine can come from two sources: transport of intracellular adenosine and hydrolysis of extracellular adenine nucleotides by CD73. In this study, we investigated the susceptibility of CD73-deficient C57BL/6 mice to collagen-induced arthritis (CIA), a well-established mouse model of RA. Our data demonstrated that CD73-deficient mice are significantly more susceptible to CIA than wild-type mice. CD73 deficiency resulted in an increased production of proinflammatory cytokines in the joints, increased Th1 T cell responses, and increased joint destruction. Surprisingly, this was accompanied by delayed anticollagen IgG responses, suggesting defective isotype class switching in CD73-deficient mice. Using bone marrow chimera mice, we demonstrated that CD73 expression on nonhematopoietic cells, but not on hematopoietic cells, was important for protection from CIA. We further demonstrated that administration of a selective A2A adenosine receptor agonist to CD73-deficient mice resulted in arthritis incidence similar to wild-type mice in support of a protective role for A2A signaling. Taken together, our study identifies CD73 as an important regulator of CIA in mice. It also strengthens the notion that CD73-generated adenosine by nonhematopoietic cells plays a protective role in RA and suggests that strategies able to enhance CD73 activity or expression levels may be a valid therapeutic option.

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“…It is documented that MSCs directly act in cartilage formation, as well as release trophic factors, and promote angiogenesis [9–11]. Amongst the various available sources, MSCs seem to have many advantages over their counterparts, while recent study demonstrated that synovium-derived MSCs (SMSCs), which have better chondrogenic potential compared with MSC, are gaining momentum [12–17]. Improved MRI features, histology, and better clinical outcome have been achieved in cartilage repair derived from SMSCs [14].…”

Section: Introductionmentioning

confidence: 99%

Long noncoding RNA DANCR regulates miR-1305-Smad 4 axis to promote chondrogenic differentiation of human synovium-derived mesenchymal stem cells

et al. 2017

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miRNAs have been reported to regulate cellular differentiation by modulating multiple signaling pathways. Long noncoding RNA (lnc RNA) DANCR was previously identified to be critical for the chondrogenesis of human synovium-derived mesenchymal stem cells (SMSC), however, the underlying molecular mechanism requires better understanding. Here, miRNA expression profiling in DANCR overexpressed in SMSCs identified significant down-regulation of miR-1305, which serves as a downstream target of DANCR. Notably, miR-1305 overexpression reversed DANCR-induced cell proliferation and chondrogenic differentiation of SMSCs, which suggested that miR-1305 antagonized the function of DANCR. Mechanistically, highly expressed miR-1305 resulted in the decreased expression of the TGF-β pathway member Smad4, and inhibition of miR-1305 enhanced the expression level of Smad4. Depletion of Smad4 suppressed the promotion of DANCR in cell proliferation and chondrogenesis of SMSCs. Collectively, our results characterized miR-1305-Smad4 axis as a major downstream functional mechanism of lncRNA DANCR in promoting the chondrogenesis in SMSCs.

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Chondrogenic potentials of human synovium-derived cells sorted by specific surface markers

Abstract: Our study suggests that CD105 and CD166 would be valuable surface markers associated with chondrogenic potential; thus, CD105- and CD166-enriched cells derived from human synovium would be practical and valuable sources for cartilage regeneration.

Cited by 36 publications

References 33 publications

Influence of Bovine Serum Lipids and Fetal Bovine Serum on the Expression of Cell Surface Markers in Cultured Bovine Preadipocytes

Influence of Bovine Serum Lipids and Fetal Bovine Serum on the Expression of Cell Surface Markers in Cultured Bovine Preadipocytes

CD73 Plays a Protective Role in Collagen-Induced Arthritis

Long noncoding RNA DANCR regulates miR-1305-Smad 4 axis to promote chondrogenic differentiation of human synovium-derived mesenchymal stem cells

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