Chondroitin‐4‐Sulfate: A Bioactive Macromolecule to Foster Vascular Healing around Stent‐Grafts after Endovascular Aneurysm Repair

Charbonneau, Cindy; Gautrot, Julien E.; Hébert, Marie‐Josée; Zhu, Xuhai; Lerouge, Sophie

doi:10.1002/mabi.200700008

Cited by 11 publications

(27 citation statements)

References 41 publications

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“…C4S and CD44 are known to contribute to cellular adhesion (Charbonneau et al , 2007; Murai et al , 2004). Our THP-1 cell adhesion assay mimics the co-expression of CD44v7 and osteopontin in active areas of active inflammation in Gottron’s papules of dermatomyositis, and its results demonstrate the adhesive interactions regulated by stretch-induced CD44v7 on dermal fibroblasts complexed with osteopontin.…”

Section: Discussionmentioning

confidence: 99%

Gottron's Papules Exhibit Dermal Accumulation of CD44 Variant 7 (CD44v7) and Its Binding Partner Osteopontin: A Unique Molecular Signature

Kim

Bashir

Werth

2012

Journal of Investigative Dermatology

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The accumulated mucin in non-Gottron’s dermatomyositis (DM) lesions is primarily chondroitin-4-sulfate (C4S), which is immunomodulatory in vitro. Gottron’s papules are a particularly resistant manifestation of DM that often persist after other lesions have resolved with therapy. We examined non-Gottron’s DM lesions and Gottron’s papule skin biopsies for C4S, CD44v7, a CS-binding isoform causally implicated in autoimmunity, and osteopontin, a CD44v7 ligand implicated in chronic inflammation. Gottron’s papule dermis contained more C4S and CD44v7 than non-Gottron’s lesions. Normal skin showed less CD44v7 over joints relative to Gottron’s lesions. All DM dermis had increased osteopontin compared to healthy skin. Mechanically stretching cultured fibroblasts for six hours induced CD44v7 mRNA and protein, while IFN-γ treatment induced OPN mRNA and protein. Osteopontin alone did not induce CD44v7, but stretching dermal fibroblasts in the presence of osteopontin increased THP-1 monocyte binding, which is blunted by anti-CD44v7 blocking antibody. C4S, CD44v7, and osteopontin are three molecules uniquely present in Gottron’s papules that contribute to inflammation individually and in association with one another. We propose that stretch-induced CD44v7 over joints, in concert with dysregulated osteopontin levels in the skin of DM patients, increases local inflammatory cell recruitment and contributes to the pathogenesis and resistance of Gottron’s papules.

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Section: Discussionmentioning

confidence: 99%

Gottron's Papules Exhibit Dermal Accumulation of CD44 Variant 7 (CD44v7) and Its Binding Partner Osteopontin: A Unique Molecular Signature

Kim

Bashir

Werth

2012

Journal of Investigative Dermatology

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“…26,27 A possible strategy to overcome this problem would be to formulate coatings that can promote optimal attachment and growth of endothelial cells on the stent surface, thereby ensuring hematocompatibility. 28,29 For this purpose, the class of polysaccharide molecules known as glycosaminoglycans holds particular promise [12][13][14][15][16] because many of these molecules are known to exert a positive stimulatory effect on angiogenesis and endothelial function. For example, hyaluronan has been widely utilized in tissue engineering applications to promote both angiogenesis and wound-healing at the same time.…”

Section: Discussionmentioning

confidence: 99%

“…9,10 Although there have already been several studies on endothelial cell interaction with various glycosaminoglycan coatings [42][43][44] and other multilayer polyelectolyte films, [45][46][47] there has not yet been a systematic comparison of the adhesion, proliferation, and gene expression profile of endothelial cells on various glycosaminoglycan-coated surfaces. Given the vast potential of glycosaminoglycan-based multilayered polyelectrolyte films in the fabrication of stent coatings [12][13][14][15][16][17][18] and other tissue engineering applications, [8][9][10][11] it is of great interest to determine which particular combination of glycosaminoglycans with other polyelectrolytes ͑i.e., chitosan, poly-L-lysine͒ would yield the most conducive substrata for endothelial cell adhesion, proliferation, and expression of differentiated phenotype.…”

Section: Discussionmentioning

confidence: 99%

“…11 Such biologically relevant properties would certainly be useful for incorporation within the coatings of coronary drug-eluting stents. Indeed, there have been numerous studies on the formulation of stent coatings with various combinations of heparin, hyaluronan, and chondroitin sulfate, [12][13][14][15][16] together with chitosan 17 and poly-L-lysine. 18 Hence, in this study, we fabricated bilayered polyelectrolyte coatings of various combinations of negatively charged glycosaminoglycans overlaid on either positively charged poly-L-lysine or chitosan on tissue culture polystyrene ͑TCPS͒ surface.…”

Section: Introductionmentioning

confidence: 99%

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Adhesion, proliferation, and gene expression profile of human umbilical vein endothelial cells cultured on bilayered polyelectrolyte coatings composed of glycosaminoglycans

et al. 2010

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This study characterized human umbilical vein endothelial cell ͑HUVEC͒ adhesion, proliferation, and gene expression on bilayered polyelectrolyte coatings composed of an outermost layer of glycosaminoglycans ͑hyaluronan, heparin, or chondroitin sulfate͒, with an underlying layer of poly-L-lysine or chitosan. The proportion of cells that adhered to the various polyelectrolyte coatings after 1 and 2 h incubations was quantified by the WST-8 assay. Interchanging poly-L-lysine with chitosan resulted in significant differences in cellular adhesion to the outermost glycosaminoglycan layer after 1 h, but these differences became insignificant after 2 h. The proliferation of HUVEC on the various bilayered polyelectrolyte coatings over 10 days was characterized using the WST-8 assay. Regardless of whether the underlying layer was poly-L-lysine or chitosan, HUVEC proliferation on the hyaluronan outermost layer was significantly less than on heparin or chondroitin sulfate. Additionally, it was observed that there was more proliferation with poly-L-lysine as the underlying layer, compared to chitosan. Subsequently, real-time polymerase chain reaction was used to analyze the expression of seven genes related to adhesion, migration, and endothelial function ͑VWF, VEGFR, VEGFA, endoglin, integrin-␣5, ICAM1, and ICAM2͒ by HUVEC cultured on the various bilayered polyelectrolyte coatings for 3 days. With poly-L-lysine as the underlying layer, biologically significant differences ͑greater than twofold͒ in the expression of VWF, VEGFR, VEGFA, endoglin, and ICAM1 were observed among the three glycosaminoglycans. With chitosan as the underlying layer, all three glycosaminoglycans displayed biologically significant differences in the expression of VWF and VEGFR compared to the chitosan control. CT-HA displayed the highest level of expression of VWF, whereas expression levels of VEGFR were almost similar among the three glycosaminoglycans.

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“…Thus, a low‐pressure plasma‐polymerized primary amine‐rich coating, “L‐PPE:N,” developed in our laboratory, has proven very efficient in promoting the adhesion and proliferation of U937 monocytes, of vascular smooth muscle cells (VSMCs) and of endothelial cells (EC), as well as for improving resistance of the latter to shear stress. Amine functional groups also widely serve to covalently graft other biologically active molecules …”

Section: Introductionmentioning

confidence: 99%

Characterization and comparison of N‐, O‐, and N+O‐functionalized polymer surfaces for efficient (HUVEC) endothelial cell colonization

Boespflug

Maire

Crescenzo

et al. 2016

Plasma Processes & Polymers

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Surface modifications are often required to enhance cell adhesion and growth around implanted biomaterials. This study compares various functionalization processes in their ability to create high densities of oxygen‐ and/or nitrogen‐containing functional groups, mostly on a polymeric biomaterial, polyethylene terephthalate (PET). Primary amine (NH2)‐rich surfaces were prepared by low‐pressure plasma‐polymerization (L‐PPE:N), plasma modification (functionalized PET, “PETf”), chemical vapour deposition (Parylene diX AM), and grafting of polyallylamine (PAAm). Plasma polymerization was also used to obtain oxygen‐rich (L‐PPE:O) as well as hybrid (L‐PPE:O,N) films, which were respectively compared to oxygen‐rich tissue culture polystyrene (TCP) and hybrid (Primaria™) culture plates. Compositions and bond types were studied by X‐ray photoelectron spectroscopy. Finally, the effect of each surface on cell adhesion and growth was assessed using human umbilical vein endothelial cells (HUVECs). Amine‐containing surfaces manifested a wide [NH2] range, up to 8.9%. Hybrid surfaces, Primaria™ and L‐PPE:O,N, showed lower [NH2] in spite of high [N], suggesting more varied and complex functionalities. Except for Parylene, all O‐ and NH2‐rich surfaces promoted HUVEC adhesion and growth similarly, despite differing chemical compositions. Primaria™ showed the best cell behavior, but L‐PPE:O,N did not reproduce this apparent synergistic effect. To conclude, both N‐ and O‐rich surfaces displayed good cell‐colonization properties, particularly plasma polymers, while “hybrid” surfaces appear somewhat ambiguous and call for further investigation.

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Chondroitin‐4‐Sulfate: A Bioactive Macromolecule to Foster Vascular Healing around Stent‐Grafts after Endovascular Aneurysm Repair

Cited by 11 publications

References 41 publications

Gottron's Papules Exhibit Dermal Accumulation of CD44 Variant 7 (CD44v7) and Its Binding Partner Osteopontin: A Unique Molecular Signature

Gottron's Papules Exhibit Dermal Accumulation of CD44 Variant 7 (CD44v7) and Its Binding Partner Osteopontin: A Unique Molecular Signature

Adhesion, proliferation, and gene expression profile of human umbilical vein endothelial cells cultured on bilayered polyelectrolyte coatings composed of glycosaminoglycans

Characterization and comparison of N‐, O‐, and N+O‐functionalized polymer surfaces for efficient (HUVEC) endothelial cell colonization

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