Chondroitin sulfate immobilized PCL nanofibers enhance chondrogenic differentiation of mesenchymal stem cells

Meghdadi, Marziyeh; Pezeshki‐Modaress, Mohamad; Irani, Shiva; Atyabi, Seyed Mohammad; Zandi, Mojgan

doi:10.1016/j.ijbiomac.2019.06.061

Cited by 35 publications

(29 citation statements)

References 44 publications

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“…CS/PdBT hydrogels, on the other hand, may not support these early chondrogenic processes and cell–cell interactions as directly, and given their delayed onset of sGAG synthesis, may be more effective at promoting mid/late stage chondrogenic matrix deposition. The longer culture time required for promotion of sGAG synthesis by CS/PdBT is also consistent with other CS‐based constructs, which traditionally show an onset around 14–28 days of culture (Agrawal & Pramanik, ; Meghdadi, Pezeshki‐Modaress, Irani, Atyabi, & Zandi, ; Sawatjui et al, ). The lack of hypertrophic calcification within CS/PdBT and NC/PdBT hydrogels is beneficial given that many conventional methods of chondrogenic induction, such as transforming growth factor‐β supplementation, produce unwanted conversion of hypertrophic chondrocytes to osteoblasts (Deng et al, ).…”

Section: Discussionsupporting

confidence: 82%

“…For the osteogenic hydrogels, all conditions showed comparable expression of chondrogenic markers at day 7, but BMHP1/PdBT (High) and GHK/PdBT (High) showed a significant drop in chondrogenic CDH2 expression at day 35 (Figure 3c). The longer culture time required for promotion of sGAG synthesis by CS/PdBT is also consistent with other CS-based constructs, which traditionally show an onset around 14-28 days of culture(Agrawal & Pramanik, 2019;Meghdadi, Pezeshki-Modaress, Irani, Atyabi, & Zandi, 2019;Sawatjui et al, 2015). In terms of osteogenic marker expression, BMHP1/PdBT (High) and both GHK/PdBT formulations showed significantly higher RUNX2 and OPN expression compared to the PdBT control at day 7, but these differences became nonsignificant at day 35 (Figure 3d).3.3 | Histological imaging of hydrogelsHistological sections of all chondrogenic and osteogenic hydrogels were stained and imaged for cells (red/purple), sGAG deposition (blue), and mineralization (brown/black) after 35 days of culture to visualize ECM and assess tissue-specific matrix deposition (Figure 4).…”

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confidence: 83%

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Click functionalized, tissue‐specific hydrogels for osteochondral tissue engineering

Guo

Kim

et al. 2019

J Biomedical Materials Res

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Osteochondral repair requires the induction of both articular cartilage and subchondral bone development, necessitating the presentation of multiple tissue-specific cues for these highly distinct tissues. To provide a singular hydrogel system for the repair of either tissue type, we have developed biofunctionalized, mesenchymal stem cell-laden hydrogels that can present in situ biochemical cues for either chondrogenesis or osteogenesis by simple click modification of a crosslinker, poly(glycolic acid)-poly(ethylene glycol)-poly(glycolic acid)-di(but-2-yne-1,4-dithiol) (PdBT). After modifying PdBT with either cartilage-specific biomolecules (N-cadherin peptide, chondroitin sulfate) or bone-specific biomolecules (bone marrow homing peptide 1, glycine-histidine-lysine peptide), the biofunctionalized, PdBT-crosslinked hydrogels can selectively promote the desired bone-or cartilage-like matrix synthesis and tissue-specific gene expression, with effects dependent on both biomolecule selection and concentration. Our findings establish the versatility of this click functionalized hydrogel system as well as its ability to promote in vitro development of osteochondral tissue phenotypes.

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Section: Discussionsupporting

confidence: 82%

supporting

confidence: 83%

Click functionalized, tissue‐specific hydrogels for osteochondral tissue engineering

Guo

Kim

et al. 2019

J Biomedical Materials Res

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“…As for CMC-OCS scaffold, about 17% of PB-MSCs were positive cells of COL-2 after culturing in vitro for 14 days, and COL-2 content also increased as time extended, indicating that CMC-OCS hydrogel could partly promote chondrogenic differentiation of PB-MSCs. The chondroitin sulfate in hydrogels probably contribute to the proliferation and chondrogenic differentiation of seed cells and the synthesis of extracellular matrix (Meghdadi et al, 2019).…”

Section: Resultsmentioning

confidence: 99%

Low-Molecular-Weight Heparin-Functionalized Chitosan-Chondroitin Sulfate Hydrogels for Controlled Release of TGF-β3 and in vitro Neocartilage Formation

et al. 2019

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Repair of hyaline cartilage remains a huge challenge in clinic because of the avascular and aneural characteristics and the paucity of endogenous repair cells. Recently, tissue engineering technique, possessing unique capacity of repairing large tissue defects, avoiding donor complications and two-stage invasive surgical procedures, has been developed a promising therapeutic strategy for cartilage injury. In this study, we incorporated low-molecular-weight heparin (LMWH) into carboxymethyl chitosan-oxidized chondroitin sulfate (CMC-OCS) hydrogel for loading transforming growth factor-β3 (TGF-β3) as matrix of peripheral blood mesenchymal stem cells (PB-MSCs) to construct tissue-engineered cartilage. Meanwhile, three control hydrogels with or without LMWH and/or TGF-β3 were also prepared. The gelling time, microstructures, mechanical properties, degradation rate, cytotoxicity, and the release of TGF-β3 of different hydrogels were investigated. In vitro experiments evaluated the tri-lineage differentiation potential of PB-MSCs, combined with the proliferation, distribution, viability, morphology, and chondrogenic differentiation. Compared with non-LMWH-hydrogels, LMWH-hydrogels (LMWH-CMC-OCS-TGF-β3) have shorter gelling time, higher mechanical strength, slower degradation rate and more stable and lasting release of TGF-β3. After two weeks of culture in vitro, expression of cartilage-specific genes collagen type-2 (COL-2) and aggrecan (AGC), and secretion of glycosaminoglycan (GAG), and COL-2 proteins in LMWH-CMC-OCS-TGF-β3 group were significantly higher than those in other groups. COL-2 immunofluorescence staining showed that the proportion of COL-2 positive cells and immunofluorescence intensity in LMWH-CMC-OCS-TGF-β3 hydrogel were significantly higher than those in other groups. The LMWH-CMC-OCS-TGF-β3 hydrogel can slowly release TGF-β3 in a long term, and meanwhile the hydrogel can provide a biocompatible microenvironment for the growth and chondrogenic differentiation of PB-MSCs. Thus, LMWH functionalized CMC-OCS hydrogels proposed in this work will be beneficial for constructing functional scaffolds for tissue-engineered cartilage.

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“…Chondroitin sulfate is similar with hyaluronan in fundamental structural and biological processes. It has been reported to be beneficial in reducing pain and functional limitation associated with knee osteoarthritis [ 89 ] and improving the mechanical and signaling properties in composite materials for cartilage engineering [ 88 , 153 , 154 ]. Alginate, agarose, chitosan and gellan gum are all natural polysaccharides with similar structures to GAGs and thus have been widely explored in cartilage and osteochondral tissue engineering.…”

Section: Strategies Of the Scaffolds For Cartilage And Osteochondral Tissue Engineeringmentioning

confidence: 99%

“…It is worth mentioning that Caplan, the author of the paper first using this term, and some other researchers have recently called for a name change of mesenchymal stem cells to more precise labels in order to reflect the function more accurately and clear up the mess [ 298 , 299 ]. The encapsulation of BMSCs in the scaffolds have been broadly studied in various materials for cartilage and osteochondral regeneration [ 149 , 154 , 174 , 190 , 234 , 270 ]. Zhou et al have evaluated the proliferation and differentiation of BMSCs into chondrocytes and osteoblasts on the two separate layers of the collagen-HAp biphasic scaffold in the presence of chondrogenic and osteogenic chemical factors [ 165 ].…”

Section: Other Key Elements In Cartilage and Osteochondral Tissue Engineeringmentioning

confidence: 99%

Articular cartilage and osteochondral tissue engineering techniques: Recent advances and challenges

Wei

Dai

2021

Bioactive Materials

194

161

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In spite of the considerable achievements in the field of regenerative medicine in the past several decades, osteochondral defect regeneration remains a challenging issue among diseases in the musculoskeletal system because of the spatial complexity of osteochondral units in composition, structure and functions. In order to repair the hierarchical tissue involving different layers of articular cartilage, cartilage-bone interface and subchondral bone, traditional clinical treatments including palliative and reparative methods have showed certain improvement in pain relief and defect filling. It is the development of tissue engineering that has provided more promising results in regenerating neo-tissues with comparable compositional, structural and functional characteristics to the native osteochondral tissues. Here in this review, some basic knowledge of the osteochondral units including the anatomical structure and composition, the defect classification and clinical treatments will be first introduced. Then we will highlight the recent progress in osteochondral tissue engineering from perspectives of scaffold design, cell encapsulation and signaling factor incorporation including bioreactor application. Clinical products for osteochondral defect repair will be analyzed and summarized later. Moreover, we will discuss the current obstacles and future directions to regenerate the damaged osteochondral tissues.

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Chondroitin sulfate immobilized PCL nanofibers enhance chondrogenic differentiation of mesenchymal stem cells

Cited by 35 publications

References 44 publications

Click functionalized, tissue‐specific hydrogels for osteochondral tissue engineering

Click functionalized, tissue‐specific hydrogels for osteochondral tissue engineering

Low-Molecular-Weight Heparin-Functionalized Chitosan-Chondroitin Sulfate Hydrogels for Controlled Release of TGF-β3 and in vitro Neocartilage Formation

Articular cartilage and osteochondral tissue engineering techniques: Recent advances and challenges

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