Chondroitin Sulphate and Glucosamine Sulphate Synergistically Reduced DRG Proinflammatory Molecules and Improved Axonal Function in Sciatic Nerve Ligated Rats.

Abstract

Neuropathic pain (NP) is a sickness of the somatosensory nervous system. It is linked to defective quality of life and often poorly managed. Due to the limited number of approved drugs, limited efficacy and side effects associated with them, drugs or drug combinations with great efficacy and very minimal or no side effects will be of great advantage in managing NP. This study aimed at investigating the synergistic antinociceptive effects of the combination of glucosamine sulphate (GS) (240mg/kg) and chondroitin sulphate (CS) (900mg/kg) in chronic constriction injury (CCI)-induced neuropathy in rat. Forty-two Wistar rats were randomly distributed into seven groups (n=6). Sciatic nerve was ligated with four loose ligatures to induce NP. Effects of drugs were examined on stimulus and non-stimulus evoked potentials, expression of dorsal root ganglia (DRG) pain modulators and structural architecture of DRG. Oral administration of GS and CS for 21 days reduced hyperalgesia, allodynia, sciatic nerve functional aberration and DRG pain modulators. Histopathology and immunohistochemistry revealed restoration of structural integrity of DRG. Our result showed that the combination of GS and CS produced antinociceptive effects by attenuating hyperalgesia, allodynia and downregulation of NP mediators. GS and CS additionally produced synergistic analgesic effect than its individual components.