Choosing the right exit: How functional plasticity of the nuclear pore drives selective and efficient <scp>mRNA</scp> export

Bensidoun, Pierre; Zenklusen, Daniel; Oeffinger, Marlene

doi:10.1002/wrna.1660

Cited by 20 publications

(13 citation statements)

References 134 publications

(231 reference statements)

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“…These and other findings collectively highlight the role of the NB in tethering the genome and regulating its activity. Moreover, TPR and Mlps are also capable of recruiting mRNPs and the TREX-2 (Transcription Elongating and RNA Export) mRNA export complex, presumably to facilitate mRNA processing and export following active transcription at the NB [27][28][29]. Supporting this notion, mutations in TPR and Mlp1/2p lead to nuclear retention of mis-spliced or aberrant mRNAs [30][31][32].…”

Section: Introductionmentioning

confidence: 92%

GBPL3 localizes to the nuclear pore complex and functionally connects the nuclear basket with the nucleoskeleton in plants

Tang

Ho²,

Kang³

et al. 2022

PLoS Biol

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The nuclear basket (NB) is an essential structure of the nuclear pore complex (NPC) and serves as a dynamic and multifunctional platform that participates in various critical nuclear processes, including cargo transport, molecular docking, and gene expression regulation. However, the underlying molecular mechanisms are not completely understood, particularly in plants. Here, we identified a guanylate-binding protein (GBP)-like GTPase (GBPL3) as a novel NPC basket component in Arabidopsis. Using fluorescence and immunoelectron microscopy, we found that GBPL3 localizes to the nuclear rim and is enriched in the nuclear pore. Proximity labeling proteomics and protein-protein interaction assays revealed that GBPL3 is predominantly distributed at the NPC basket, where it physically associates with NB nucleoporins and recruits chromatin remodelers, transcription apparatus and regulators, and the RNA splicing and processing machinery, suggesting a conserved function of the NB in transcription regulation as reported in yeasts and animals. Moreover, we found that GBPL3 physically interacts with the nucleoskeleton via disordered coiled-coil regions. Simultaneous loss of GBPL3 and 1 of the 4 Arabidopsis nucleoskeleton genes CRWNs led to distinct development- and stress-related phenotypes, ranging from seedling lethality to lesion development, and aberrant transcription of stress-related genes. Our results indicate that GBPL3 is a bona fide component of the plant NPC and physically and functionally connects the NB with the nucleoskeleton, which is required for the coordination of gene expression during plant development and stress responses.

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Section: Introductionmentioning

confidence: 92%

GBPL3 localizes to the nuclear pore complex and functionally connects the nuclear basket with the nucleoskeleton in plants

Tang

Ho²,

Kang³

et al. 2022

PLoS Biol

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“…This is likely a consequence of regulatory proteins binding to the 3 UTR and slowing export or actively retaining mRNA transcripts within the nucleus. Such mechanisms are likely to occur also in yeast, where nuclear traffic is highly regulated [17].…”

Section: Cis-regulatory Featuresmentioning

confidence: 99%

Seeking a Role for Translational Control by Alternative Polyadenylation in Saccharomyces cerevisiae

Turner

2021

Microorganisms

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Alternative polyadenylation (APA) represents an important mechanism for regulating isoform-specific translation efficiency, stability, and localisation. Though some progress has been made in understanding its consequences in metazoans, the role of APA in the model organism Saccharomyces cerevisiae remains a relative mystery because, despite abundant studies on the translational state of mRNA, none differentiate mRNA isoforms’ alternative 3′-end. This review discusses the implications of alternative polyadenylation in S. cerevisiae using other organisms to draw inferences. Given the foundational role that research in this yeast has played in the discovery of the mechanisms of cleavage and polyadenylation and in the drivers of APA, it is surprising that such an inference is required. However, because advances in ribosome profiling are insensitive to APA, how it impacts translation is still unclear. To bridge the gap between widespread observed APA and the discovery of any functional consequence, we also provide a review of the experimental techniques used to uncover the functional importance of 3′ UTR isoforms on translation.

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“…However, recent findings in both animals and yeast have shown that the NPC plays a role in both active and repressive activities, with certain NPC components or subcomplexes preferentially affecting gene activation or silencing [ 13 ]. Interestingly, the nuclear pore exhibits high heterogeneity at distinct nuclear subdomains even in a single cell, which might be required for organization of chromatin compartmentalization and transport of specific cargos [ 96 ]. Taken together, the NPC is clearly a dynamic assembly, with both highly flexible composition and conformation, that accommodates its roles in cargo transport, genome activities and beyond.…”

Section: The Npc Bridges Nuclear Transport and Gene Regulationmentioning

confidence: 99%

Plant nuclear envelope as a hub connecting genome organization with regulation of gene expression

Tang¹

2023

Nucleus

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Eukaryotic cells organize their genome within the nucleus with a double-layered membrane structure termed the nuclear envelope (NE) as the physical barrier. The NE not only shields the nuclear genome but also spatially separates transcription from translation. Proteins of the NE including nucleoskeleton proteins, inner nuclear membrane proteins, and nuclear pore complexes have been implicated in interacting with underlying genome and chromatin regulators to establish a higher-order chromatin architecture. Here, I summarize recent advances in the knowledge of NE proteins that are involved in chromatin organization, gene regulation, and coordination of transcription and mRNA export. These studies support an emerging view of plant NE as a central hub that contributes to chromatin organization and gene expression in response to various cellular and environmental cues.

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Choosing the right exit: How functional plasticity of the nuclear pore drives selective and efficient mRNA export

Cited by 20 publications

References 134 publications

GBPL3 localizes to the nuclear pore complex and functionally connects the nuclear basket with the nucleoskeleton in plants

GBPL3 localizes to the nuclear pore complex and functionally connects the nuclear basket with the nucleoskeleton in plants

Seeking a Role for Translational Control by Alternative Polyadenylation in Saccharomyces cerevisiae

Plant nuclear envelope as a hub connecting genome organization with regulation of gene expression

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