Chorionic gonadotropin stimulates maternal hepatocyte proliferation during pregnancy

Cho, Jaeyong; Tsugawa, Yoji; Imai, Yumi; Imai, Takeshi

doi:10.1016/j.bbrc.2021.09.039

Cited by 3 publications

(9 citation statements)

References 18 publications

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“…The following antibodies were purchased: β-actin (sc-47778, Santa Cruz Biotechnology, Santa Cruz, CA, USA), FLAG (F-1804, Sigma, St. Louis, MO, USA), and UDP-glucose pyrophosphorylase (UGP, sc-377089 Santa Cruz). Mouse pancreas-derived NIT-1 cells (CRL-2055TM purchased from ATCC, Manassas, VA, USA) were maintained as described previously [ 1 , 14 , 15 , 16 , 17 , 18 , 19 , 20 , 21 ]. Briefly, the cells were plated at a density of 1.5–3.0 × 10 6 cells/60 mm dish with replacement of F-12K medium (Kaighn’s modification of Ham’s F-12 medium) plus 10% fetal calf serum (FCS) after 48 h of culture.…”

Section: Methodsmentioning

confidence: 99%

“…Briefly, the cells were plated at a density of 1.5–3.0 × 10 6 cells/60 mm dish with replacement of F-12K medium (Kaighn’s modification of Ham’s F-12 medium) plus 10% fetal calf serum (FCS) after 48 h of culture. Human embryo kidney 293T (HEK293T) cells and human hepatocellular carcinoma (Hep G2) cells were maintained with DMEM + 10% FCS [ 20 , 21 , 22 , 23 ]. Cereblon KO cells were kindly provided by Tokyo Medical University [ 8 ].…”

Section: Methodsmentioning

confidence: 99%

“…Insulin secretion was determined using a commercial enzyme-linked immunosorbent assay (ELISA) kit (Shibayagi, Gunma, Japan) [ 15 , 16 , 17 , 18 , 19 , 20 , 21 , 22 ] as described previously. Briefly, the culture medium of the NIT-1 cells was replaced with UDP-glucose-containing F12K + 10% fetal calf serum (FCS) for the indicated time.…”

Section: Methodsmentioning

confidence: 99%

“…Immunoprecipitation and Western blot analysis were performed as described previously with minor modifications [ 14 , 15 , 16 , 17 , 18 , 19 , 20 , 21 ]. NIT-1 cells transfected with the pCDNA-insulin-Myc expression vector were cultivated in arginine-free medium for 30 min before arginine was added.…”

Section: Methodsmentioning

confidence: 99%

“…Signals were quantified with the LAS 4000 imaging system (GE Healthcare). ImageJ was used for densitometry of scanned membranes [ 14 , 15 , 16 , 17 , 18 , 19 , 20 , 21 ].…”

Section: Methodsmentioning

confidence: 99%

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UDP-Glucose: A Cereblon-Dependent Glucokinase Protein Degrader

Cho

Miyagawa

Yamaguchi

et al. 2022

IJMS

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We previously reported that glucokinase is ubiquitinated and degraded by cereblon with an unknown endogenous glucokinase protein degrader. Here, we show that UDP-glucose is a glucokinase protein degrader. We identified that both glucose and UDP-glucose bind to glucokinase and that both uridine and UDP-glucose bind to cereblon in a similar way to thalidomide. From these results, UDP-glucose was identified as a molecular glue between cereblon and glucokinase. Glucokinase produces glucose-6-phosphate in the pancreas and liver. Especially in β-cells, glucokinase is the main target of glucose for glucose-induced insulin secretion. UDP-glucose administration ubiquitinated and degraded glucokinase, lowered glucose-6-phosphate production, and then reduced insulin secretion in β-cell lines and mice. Maturity-onset diabetes of the young type 2 (MODY2) glucokinaseE256K mutant protein was resistant to UDP-glucose induced ubiquitination and degradation. Taken together, glucokinase ubiquitination and degradation signaling might be impaired in MODY2 patients.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…Signals were quantified with the LAS 4000 imaging system (GE Healthcare). ImageJ was used for densitometry of scanned membranes [ 14 , 15 , 16 , 17 , 18 , 19 , 20 , 21 ].…”

Section: Methodsmentioning

confidence: 99%

See 3 more Smart Citations

UDP-Glucose: A Cereblon-Dependent Glucokinase Protein Degrader

Cho

Miyagawa

Yamaguchi

et al. 2022

IJMS

Self Cite

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Histomorphology of the ovaries of rabbits does during ovulation induced by the combined use of gonadotropins

Tverdokhlib,

Naumenko,

Koshevoy

et al. 2024

Ukr. Jour. of Vet. and Agr. Sci.

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Hormonal stimulation of folliculogenesis is a general method of increasing reproductive capacity in rabbits; however, studying the safety of the developed protocols remains an urgent scientific task. The use of hormonal agents is due to the physiological characteristics of rabbits, primarily coitus-dependent mechanisms of ovulation. Applying gonadotropin-releasing analogs depends on mature follicles ready for ovulation in the ovary. It, therefore, has limited effectiveness for rabbits of the “zero” cycle that have not yet given birth. Therefore, such females are recommended to use medications based on gonadotropins obtained from the serum of foal mares (eCG) or human chorionic gonadotropin (hCG). There is limited data on the combined use of gonadotropins, especially the long-term administration in different doses. Thus, the research aimed to conduct a morphological evaluation of rabbit's ovaries with combined serum and chorionic gonadotropins to stimulate folliculogenesis before artificial insemination to assess the safety of the proposed dosages and long-term administration. Stimulation of ovulation in rabbits of experimental groups was caused by the combined use of serum and chorionic gonadotropins (eCG – 400 IU; hCG – 200 IU) for experimental groups 1 and 2 at a dose of 40 IU and 24 IU, respectively. Females of the control group were induced to ovulate by subcutaneous injection of 0.2 ml of gonadotropin-releasing hormone analog. Ovaries were collected on the seventh day of gestation after the euthanasia of the rabbit with prior premedication. After dissection and macroscopic examination, the ovaries were fixed in a 10% aqueous solution of neutral formalin. Histological slides were made according to the general method by pouring in paraffin and subsequent staining with hematoxylin and eosin. The research has established that repeated (during five reproductive cycles) combined use of serum and chorionic gonadotropins has dose-dependent effectiveness and can cause dysfunction of ovaries and blood circulation. Pathological changes may acquire a systemic character with the manifestation of critical conditions for the health and life of rabbits. The stimulating effect of the combined use of eCG and hCG was detected at a dose of 40 IU, and the inhibition of folliculogenesis was observed at 24 IU. Since the premises for the hyperandrogenism were found microscopically, it was essential to investigate the level of androgens in the blood of rabbits after using a dose of 24 IU. At the same time, both doses of gonadotropins affect the reactivity of the endothelium, which is manifested by the presence of intravascular vesicles and changes in the rheological properties of blood.

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Maternal hepatic immunology during pregnancy

et al. 2023

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The liver plays pivotal roles in immunologic responses, and correct hepatic adaptations in maternal immunology are required during pregnancy. In this review, we focus on anatomical and immunological maternal hepatic adaptations during pregnancy, including our recent reports in this area. Moreover, we summarize maternal pregnancy-associated liver diseases, including hyperemesis gravidarum; intrahepatic cholestasis of pregnancy; preeclampsia, specifically hemolysis, elevated liver enzymes, and low platelet count syndrome; and acute fatty liver of pregnancy. In addition, the latest information about the factors that regulate hepatic immunology during pregnancy are reviewed for the first time, including human chorionic gonadotropin, estrogen, progesterone, growth hormone, insulin like growth factor 1, oxytocin, adrenocorticotropic hormone, adrenal hormone, prolactin, melatonin and prostaglandins. In summary, the latest progress on maternal hepatic anatomy and immunological adaptations, maternal pregnancy-associated diseases and the factors that regulate hepatic immunology during pregnancy are discussed, which may be used to prevent embryo loss and abortion, as well as pregnancy-associated liver diseases.

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Chorionic gonadotropin stimulates maternal hepatocyte proliferation during pregnancy

Cited by 3 publications

References 18 publications

UDP-Glucose: A Cereblon-Dependent Glucokinase Protein Degrader

UDP-Glucose: A Cereblon-Dependent Glucokinase Protein Degrader

Histomorphology of the ovaries of rabbits does during ovulation induced by the combined use of gonadotropins

Maternal hepatic immunology during pregnancy

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