Chorismate Pyruvate-Lyase and 4-Hydroxy-3-solanesylbenzoate Decarboxylase Are Required for Plastoquinone Biosynthesis in the Cyanobacterium Synechocystis sp. PCC6803

Pfaff, Christian; Glindemann, Niels; Gruber, Jens; Frentzen, Margrit; Sadre, Radin

doi:10.1074/jbc.m113.511709

Cited by 23 publications

(33 citation statements)

References 45 publications

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“…Sadre et al also examined properties of Slr0926 product isolated from transgenic E. coli and proved that the transgenic protein indeed was HBA prenyltransferase (Sadre et al 2012). Using an analogical approach, the same group confirmed that cyanobacterial homologues of UbiC, UbiD and UbiX, encoding chorismate lyase and two 4-hydroxy-3-solanesylbenzoate decarboxylases, respectively, participate in PQ biosynthesis (Pfaff et al 2014).…”

Section: Introductionmentioning

confidence: 77%

“…PCC 6803 was used for experiments concerning a-Toc and PQ biosynthesis more recently (Dahnhardt et al 2002;Sadre et al 2012;Pfaff et al 2014), these species were used also in our experiments. Two other species chosen were: Thermosynechococcus elongatus, in which there are no close homologues of genes encoding enzymes of plant a-Toc and PQ biosynthetic pathway and Synechococcus sp.…”

Section: Resultsmentioning

confidence: 99%

“…As in early research carried out by Whistance and Threlfall (Whistance and Threlfall 1970), radiolabelled HGA was incorporated into PQ, whereas in latest research (Sadre et al 2012;Pfaff et al 2014), the pathway analogical to UQ biosynthetic pathway present in proteobacteria, in which p-hydroxybenzoate (HBA) serves as a precursor of PQ head group was proved to occur in Synechocystis sp. PCC 6803, there is a need to explain these differences.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Cyanobacteria use both p-hydroxybenozate and homogentisate as a precursor of plastoquinone head group

Nowicka

Kruk

2016

Acta Physiol Plant

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Section: Introductionmentioning

confidence: 77%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Cyanobacteria use both p-hydroxybenozate and homogentisate as a precursor of plastoquinone head group

Nowicka

Kruk

2016

Acta Physiol Plant

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“…S4 and Dataset S2) (38). Recent work has shown that plastoquinone biosynthesis in cyanobacteria uses 4HB as an intermediate (39,40). Indeed, the benzoate compounds in MC1 and MC2 correlate negatively with the aromatic amino acids, which are consumed in plastoquinone biosynthesis (Fig.…”

Section: Correlations In Metabolite Abundance Can Help Classify Unknownmentioning

confidence: 99%

The circadian oscillator in Synechococcus elongatus controls metabolite partitioning during diurnal growth

Diamond

Jun

Rubin

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

124

158

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Synechococcus elongatus PCC 7942 is a genetically tractable model cyanobacterium that has been engineered to produce industrially relevant biomolecules and is the best-studied model for a prokaryotic circadian clock. However, the organism is commonly grown in continuous light in the laboratory, and data on metabolic processes under diurnal conditions are lacking. Moreover, the influence of the circadian clock on diurnal metabolism has been investigated only briefly. Here, we demonstrate that the circadian oscillator influences rhythms of metabolism during diurnal growth, even though lightdark cycles can drive metabolic rhythms independently. Moreover, the phenotype associated with loss of the core oscillator protein, KaiC, is distinct from that caused by absence of the circadian output transcriptional regulator, RpaA (regulator of phycobilisome-associated A). Although RpaA activity is important for carbon degradation at night, KaiC is dispensable for those processes. Untargeted metabolomics analysis and glycogen kinetics suggest that functional KaiC is important for metabolite partitioning in the morning. Additionally, output from the oscillator functions to inhibit RpaA activity in the morning, and kaiC-null strains expressing a mutant KaiC phosphomimetic, KaiC-pST, in which the oscillator is locked in the most active output state, phenocopies a ΔrpaA strain. Inhibition of RpaA by the oscillator in the morning suppresses metabolic processes that normally are active at night, and kaiC-null strains show indications of oxidative pentose phosphate pathway activation as well as increased abundance of primary metabolites. Inhibitory clock output may serve to allow secondary metabolite biosynthesis in the morning, and some metabolites resulting from these processes may feed back to reinforce clock timing.C yanobacteria comprise a promising engineering platform for the production of fuels and industrial chemicals. These organisms already have been engineered to produce ethanol, isobutyraldehyde, alkanes, and hydrogen (1-4). However, the efficient industrial-scale application of these photosynthetic organisms will require their growth and maintenance in the outdoors where they will be subjected to light-dark (LD) cycles (5). Phototrophic cyanobacteria present a completely different engineering challenge relative to heterotrophic bacteria such as Escherichia coli: their cellular activities respond strongly to the presence and absence of light because their metabolism is centered on photosynthesis (6, 7). Diverse cyanobacteria also possess a true circadian clock that synchronizes with external LD cycles and has been demonstrated to drive both gene expression and metabolic rhythms (8-10). It is important to understand how signals from the external environment and the internal circadian clock are integrated to modulate metabolic processes in environmentally relevant LD cycles to optimize the engineering of these organisms. In this work we attempt to separate the influences of environment and circadian control using the cyan...

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“…For instance, in Escherichia coli, the ring of ubiquinone is made from 4-hydroxybenzoate, which comes from the direct aromatization of chorismate catalyzed by chorismate pyruvate-lyase or UbiC (Figure 1; Nichols and Green, 1992). However, this enzyme appears to be restricted to the eubacterial lineage (Clarke, 2000;Kawamukai, 2009;Pfaff et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

The Origin and Biosynthesis of the Benzenoid Moiety of Ubiquinone (Coenzyme Q) in Arabidopsis

et al. 2014

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It is not known how plants make the benzenoid ring of ubiquinone, a vital respiratory cofactor. Here, we demonstrate that Arabidopsis thaliana uses for that purpose two separate biosynthetic branches stemming from phenylalanine and tyrosine. Gene network modeling and characterization of T-DNA mutants indicated that acyl-activating enzyme encoded by At4g19010 contributes to the biosynthesis of ubiquinone specifically from phenylalanine. CoA ligase assays verified that At4g19010 prefers para-coumarate, ferulate, and caffeate as substrates. Feeding experiments demonstrated that the at4g19010 knockout cannot use para-coumarate for ubiquinone biosynthesis and that the supply of 4-hydroxybenzoate, the side-chain shortened version of para-coumarate, can bypass this blockage. Furthermore, a trans-cinnamate 4-hydroxylase mutant, which is impaired in the conversion of trans-cinnamate into para-coumarate, displayed similar defects in ubiquinone biosynthesis to that of the at4g19010 knockout. Green fluorescent protein fusion experiments demonstrated that At4g19010 occurs in peroxisomes, resulting in an elaborate biosynthetic architecture where phenylpropanoid intermediates have to be transported from the cytosol to peroxisomes and then to mitochondria where ubiquinone is assembled. Collectively, these results demonstrate that At4g19010 activates the propyl side chain of para-coumarate for its subsequent b-oxidative shortening. Evidence is shown that the peroxisomal ABCD transporter (PXA1) plays a critical role in this branch.

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Chorismate Pyruvate-Lyase and 4-Hydroxy-3-solanesylbenzoate Decarboxylase Are Required for Plastoquinone Biosynthesis in the Cyanobacterium Synechocystis sp. PCC6803

Cited by 23 publications

References 45 publications

Cyanobacteria use both p-hydroxybenozate and homogentisate as a precursor of plastoquinone head group

Cyanobacteria use both p-hydroxybenozate and homogentisate as a precursor of plastoquinone head group

The circadian oscillator in Synechococcus elongatus controls metabolite partitioning during diurnal growth

The Origin and Biosynthesis of the Benzenoid Moiety of Ubiquinone (Coenzyme Q) in Arabidopsis

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