Choristoneura fumiferana granulovirus: sequence analysis and 5′ characterization of ORF891

Bah, A.; Lucarotti, Christopher J.; Arella, M.; Guertin, Claude

doi:10.1007/s007050050539

Cited by 7 publications

(4 citation statements)

References 23 publications

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“…Other GV me53 homologues show little similarity in the upstream promoter region of this gene. CfGV ORF 891 has been shown to be transcribed from an early promoter but also contains a late promoter (Bah et al ., 1999 ). The ClGV homologue also contains early and late promoter elements (Jehle & Backhaus, 1994 ) and CpGV contains early but not late promoter elements (Crook et al ., 1997 ).…”

Section: Resultsmentioning

confidence: 99%

Phylogenetic analysis of conserved genes within the ecdysteroid UDP-glucosyltransferase gene region of the slow-killing Adoxophyes orana granulovirus

Wormleaton

Winstanley

2001

Journal of General Virology

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A physical map of the genome of Adoxophyes orana granulovirus (AoGV) was constructed for the restriction enzymes BamHI, BglII, EcoRI, PstI and SacI using restriction endonuclease analysis and DNA hybridization techniques. This enabled the size of the AoGV genome to be estimated at 100n9 kbp. A plasmid library covering 99n9 % of the AoGV genome was constructed using five restriction enzymes. The ecdysteroid UDP-glucosyltransferase gene (egt) was located by hybridization with the egt gene of Cydia pomonella granulovirus. The sequence of 6000 bp of the egt region is presented and compared to the equivalent area in other GVs. Database searches showed that this region contained eight open reading frames (ORFs) similar to the baculovirus genes egt, granulin, pk-1, me53 and four ORFs of Xestia c-nigrum granulovirus (ORF 178, ORF 2, ORF 7 and ORF 8). The egt gene was shown to encode an active EGT using an EGT assay. Phylogenetic trees of the granulovirus genes egt, granulin, pk-1 and me53 were constructed using maximum parsimony and distance analyses. These analyses indicated that AoGV genes may be more closely related to other tortricid-infecting GVs than to GVs that infect other lepidopteran families.

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Section: Resultsmentioning

confidence: 99%

Phylogenetic analysis of conserved genes within the ecdysteroid UDP-glucosyltransferase gene region of the slow-killing Adoxophyes orana granulovirus

Wormleaton

Winstanley

2001

Journal of General Virology

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“…Notably, Choristoneura fumiferana granulovirus emerged as a robust biomarker for carnivorous birds, particularly prevalent in the Wader-Carnivorous, Raptor-Carnivorous, and Natatores-Carnivorous groups. This virus, affiliated with Baculoviridae , signifies the dietary preference of carnivorous birds for Lepidoptera or Hymenoptera insects [ 28 ], establishing it as a reliable biomarker.…”

Section: Discussionmentioning

confidence: 99%

The Multi-Kingdom Microbiome of Wintering Migratory Birds in Poyang Lake, China

Liu,

Li,

Song

et al. 2024

Viruses

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Wild birds are a natural reservoir for zoonotic viruses. To clarify the role of migratory birds in viruses spread in Poyang Lake, we investigated the microbiome of 250 wild bird samples from 19 species in seven orders. The bacterial and viral content abundance and diversity were preliminarily evaluated by Kraken2 and Bracken. After de novo assembly by Megahit and Vamb, viral contigs were identified by CheckV. The reads remapped to viral contigs were quantified using Bowtie2. The bacterial microbiome composition of the samples covers 1526 genera belonging to 175 bacterial orders, while the composition of viruses covers 214 species belonging to 22 viral families. Several taxonomic biomarkers associated with avian carnivory, oral sampling, and raptor migration were identified. Additionally, 17 complete viral genomes belonging to Astroviridae, Caliciviridae, Dicistroviridae, Picornaviridae, and Tombusviridae were characterized, and their phylogenetic relationships were analyzed. This pioneering metagenomic study of migratory birds in Poyang Lake, China illuminates the diverse microbial landscape within these birds. It identifies potential pathogens, and uncovers taxonomic biomarkers relevant to varied bird habitats, feeding habits, ecological classifications, and sample types, underscoring the public health risks associated with wintering migratory birds.

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“…The results reported here document EGT activity in the haemolymph of virus‐infected larvae and confirmed the presence of an egt gene in the AdhoGV genome. The AdhoGV egt and granulin genes are 1 kbp apart and are orientated in opposite directions, as in C. fumiferana GV (ChfuGV) (Bah et al ., 1999), Cryptophlebia leucotreta GV (Jehle & Backhaus, 1994) and Cydia pomonella GV (CpGV) (Luque et al ., 2001). Three candidate late‐promoter motifs and a possible TATA signal with a CATT motif are located upstream from the AdhoGV egt ORF.…”

Section: Discussionmentioning

confidence: 99%

“…The ORF of the AdhoGV egt gene showed nucleotide and amino acid sequence homology with known baculovirus egt s: 51.5% identity in a 1073 nucleotide overlap and 39.5% identity in a 458 amino acid overlap with the CpGV egt (Luque et al ., 2001; GenBank accession no. ); 54.2% identity in a 1342 nucleotide overlap and 45.5% identity in a 455 amino acid overlap with the ChfuGV egt (Bah et al ., 1999 and unpublished data; GenBank accession no. ); and 54.8% identity in a 825 nucleotide overlap and 34.1% identity in a 457 amino acid overlap with the MbMNPV egt (Clarke et al ., 1996; GenBank accession no.…”

Section: Discussionmentioning

confidence: 99%

Granulovirus prevents pupation and retards development of Adoxophyes honmai larvae

Nakai

Goto

Shiotsuki

et al. 2002

Physiological Entomology

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Abstract. Larvae of Adoxophyes honmai (Lepidoptera: Tortricidae) infected with granulovirus (AdhoGV) do not pupate; instead, they undergo prolonged larval development and die during the final stadium. Non-infected larvae, however, pupate after five larval stadia. Insect metamorphosis is regulated by fluctuations of ecdysteroid and Juvenile Hormone (JH). JH esterase activity and titres of ecdysteroid must be measured to understand fully the interaction of an insect virus and its host. JH esterase activity is consistently low in AdhoGV-infected larvae, which suggests that JH in AdhoGV-infected larvae is not degraded during the final stadium. The ecdysteroid titre in non-infected larvae showed a large peak in the final stadium before pupation, whereas that in AdhoGV-infected larvae increased from day 2 to day 5 in the final stadium, and then remained at a high level until death. Furthermore, an ecdysteroid UDP-glucosyltransferase (EGT) assay showed that this activity occurs in haemolymph from AdhoGV-infected larvae, but not in haemolymph of non-infected larvae. PCR and sequencing analysis revealed that the AdhoGV genome contains an egt gene, which encodes a protein of 445 amino acids, located approximately 1 kbp upstream from the granulin gene. These results suggest that AdhoGV-infected larvae are prevented from pupating because JHE activity is suppressed and EGT expression inactivates ecdysteroid in the haemolymph.

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Choristoneura fumiferana granulovirus: sequence analysis and 5′ characterization of ORF891

Cited by 7 publications

References 23 publications

Phylogenetic analysis of conserved genes within the ecdysteroid UDP-glucosyltransferase gene region of the slow-killing Adoxophyes orana granulovirus

Phylogenetic analysis of conserved genes within the ecdysteroid UDP-glucosyltransferase gene region of the slow-killing Adoxophyes orana granulovirus

The Multi-Kingdom Microbiome of Wintering Migratory Birds in Poyang Lake, China

Granulovirus prevents pupation and retards development of Adoxophyes honmai larvae

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