chr genes from adaptive replicons are responsible for chromate resistance by Burkholderia xenovorans LB400

Reyes-Gallegos, Rosa I.; Ramírez-Díaz, Martha I.; Cervantes, Carlos

doi:10.1007/s11274-015-1996-x

Cited by 9 publications

(6 citation statements)

References 10 publications

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“…Taken together, the ability of KJ-34 to protect against B. cinerea, combined with its ability to fix nitrogen, [can increase plant resistance] to pathogens and promote growth. Burkholderia bacteria have the ability to degrade environmental pollutants, B. xenovorans LB400 can decrease carcinogens (polychlorinated biphenyl, 2-aminophenol, Metallica) (Martıńez et al, 2007;Chirino et al, 2013;Reyes-Gallegos et al, 2016). The Burkholderia species MBA4 can enrich and bioremediate haloacids from soil (Su et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

Burkholderia gladioli strain KJ-34 exhibits broad-spectrum antifungal activity

Yang

Wang²,

Wan³

et al. 2023

Front. Plant Sci.

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IntroductionPlant pathogens are one of the major constraints on worldwide food production. The antibiotic properties of microbes identified as effective in managing plant pathogens are well documented.MethodsHere, we used antagonism experiments and untargeted metabolomics to isolate the potentially antifungal molecules produced by KJ-34.ResultsKJ-34 is a potential biocontrol bacterium isolated from the rhizosphere soil of rice and can fight multiple fungal pathogens (i.e. Ustilaginoidea virens, Alternaria solani, Fusarium oxysporum, Phytophthora capsica, Corynespora cassiicola). The favoured fermentation conditions are determined and the fermentation broth treatment can significantly inhibit the infection of Magnaporthe oryzae and Botryis cinerea. The fermentation broth suppression ratio is 75% and 82%, respectively. Fermentation broth treatment disrupted the spore germination and led to malformation of hyphae. Additionally, we found that the molecular weight of antifungal products were less than 1000 Da through semipermeable membranes on solid medium assay. To search the potentially antifungal molecules that produce by KJ-34, we used comparative and bioinformatics analyses of fermentation broth before and after optimization by mass spectrometry. Untargeted metabolomics analyses are presumed to have a library of antifungal agents including benzoylstaurosporine, morellin and scopolamine.DiscussionThese results suggest that KJ-34 produced various biological control agents to suppress multiple phytopathogenic fungi and showed a strong potential in the ecological technologies of prevention and protection.

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Section: Discussionmentioning

confidence: 99%

Burkholderia gladioli strain KJ-34 exhibits broad-spectrum antifungal activity

Yang

Wang²,

Wan³

et al. 2023

Front. Plant Sci.

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“…In the first set of experiments, we compared the growth of the wildtype and recombinant strains under different levels of Cr stress. We wondered whether the accumulation of multiple Cr transporters in B. subtilis results in a more robust extrusion of Cr(VI), as is seen in some other species ( Reyes-Gallegos et al, 2016 ). Another issue we addressed was whether ChrA, an LCHR chromate transporter, compensates for the loss of SCHR transporters YwrB and YwrA in the Δ ywrBA B. subtilis strain.…”

Section: Discussionmentioning

confidence: 99%

“…The SCHR family comprises mono-domain proteins of around 200 aa in length, while the LCHR family consists of bi-domain proteins 400 aa long. Interestingly, certain strains encode a combination of mono- and bi-domain chromate transporters in their genomes, located either on the chromosome or a plasmid, with different contributions to overall chromate resistance, as in the case for Burkholderia xenovorans ( Reyes-Gallegos et al, 2016 ). All chromate transporters typically possess multiple transmembrane segments (TMS).…”

Section: Introductionmentioning

confidence: 99%

Implantation of Bacillus pseudomycoides Chromate Transporter Increases Chromate Tolerance in Bacillus subtilis

et al. 2022

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Chromium of anthropogenic origin contaminates the environment worldwide. The toxicity of chromium, a group I human carcinogen, is greatest when it is in a hexavalent oxidation state, Cr(VI). Cr(VI) is actively transported into the cell, triggering oxidative damage intracellularly. Due to the abundance of unspecific intracellular reductants, any microbial species is capable of bio-transformation of toxic Cr(VI) to innocuous Cr(III), however, this process is often lethal. Only some bacterial species are capable of sustaining the vegetative growth in the presence of a high concentration of Cr(VI) and thus operate as self-sustainable bioremediation agents. One of the successful microbial Cr(VI) detoxification strategies is the activation of chromate efflux pumps. This work describes transplantation of the chromate efflux pump from the potentially pathogenic but highly Cr resistant Bacillus pseudomycoides environmental strain into non-pathogenic but only transiently Cr tolerant Bacillus subtilis strain. In our study, we compared the two Bacillus spp. strains harboring evolutionarily diverged chromate efflux proteins. We have found that individual cells of the Cr-resistant B. pseudomycoides environmental strain accumulate less Cr than the cells of B. subtilis strain. Further, we found that survival of the B. subtilis strain during the Cr stress can be increased by the introduction of the chromate transporter from the Cr resistant environmental strain into its genome. Additionally, the expression of B. pseudomycoides chromate transporter ChrA in B. subtilis seems to be activated by the presence of chromate, hinting at versatility of Cr-efflux proteins. This study outlines the future direction for increasing the Cr-tolerance of non-pathogenic species and safe bioremediation using soil bacteria.

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“…xenovorans LB400, which possesses six genes encoding chromate ion transporters, only the chrA2 gene present on a megaplasmid is subject to chromate-induced expression [ 35 ]. Interestingly, it was recently demonstrated that ChrA2 is the major determinant of chromate resistance in this organism [ 37 ]. The chrBACF operon of Ochrobactrum tritici 5bvl1, which is located in a chromosomally integrated transposon, proved also to be chromate-induced [ 38 ].…”

Section: Discussionmentioning

confidence: 99%

ChrASO, the chromate efflux pump of Shewanella oneidensis, improves chromate survival and reduction

et al. 2017

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The chromate efflux pump encoding gene chrASO was identified on the chromosome of Shewanella oneidensis MR1. Although chrASO is expressed without chromate, its expression level increases when Cr(VI) is added. When deleted, the resulting mutant ΔchrASO exhibits a chromate sensitive phenotype compared to that of the wild-type strain. Interestingly, heterologous expression of chrASO in E. coli confers resistance to high chromate concentration. Moreover, expression of chrASO in S. oneidensis and E. coli significantly improves Cr(VI) reduction. This effect could result either from extracytoplasmic chromate reduction or from a better cell survival leading to enhanced Cr(VI) reduction.

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chr genes from adaptive replicons are responsible for chromate resistance by Burkholderia xenovorans LB400

Cited by 9 publications

References 10 publications

Burkholderia gladioli strain KJ-34 exhibits broad-spectrum antifungal activity

Burkholderia gladioli strain KJ-34 exhibits broad-spectrum antifungal activity

Implantation of Bacillus pseudomycoides Chromate Transporter Increases Chromate Tolerance in Bacillus subtilis

ChrASO, the chromate efflux pump of Shewanella oneidensis, improves chromate survival and reduction

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