CHROMAgar Candida más fluconazol: comparación con técnicas de microdilución

Linares, María José Pedraja; Charriel, Guadalupe; Solis, Francisco J.; Casal, Manuel

doi:10.1157/13052333

Cited by 3 publications

(2 citation statements)

References 8 publications

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“…Our findings corroborate an earlier report in which C. albicans was found to be the most common isolated species that caused candidemia at the Armed Forces Hospital, Riyadh, with 50.7% cases [1]. Moreover, it is also reported by several authors that the most frequently encountered species in Candida infections is C. albicans [32,34,35].…”

Section: Discussionsupporting

confidence: 92%

Susceptibility of Fluconazole-Resistant Candida albicans to Thyme Essential Oil

Alshaikh

Perveen

2021

Microorganisms

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Candida spp. is the most common microbial pathogen in fungal infections. There has been a tremendous increase in cases of candidiasis, especially among critically ill non-neutropenic patients. Candida albicans’ isolates were procured from the Prince Sultan Military Hospital, Riyadh, KSA. The isolates were characterized for their identification using CHROMagar, carbohydrate metabolism, germ tube formation, and RAPD-PCR techniques. The essential oil of Thymus vulgaris was obtained by hydro-distillation and characterized to decipher the major bioactive phytoconstituents. The antifungal activity of the thyme essential oil (TEO) was evaluated against fluconazole-resistant C. albicans isolates. The major phytocomponents identified by GC/MS were thymol (68.1%) followed by γ-terpinene (8.9%), cymol (7.7%), caryophyllene (1.1%), linalool (1.4%). The TEO successfully reduced the growth of C. albicans isolates. At very low doses, the TEO proved to be fungi static and fungicidal. TEO also effectively inhibited the germ tube formation and budging of fungal pathogens. The time kill assays have shown that TEO was more effective against drug resistant clinical isolates than fluconazole. This study provides an array of experimental evidence regarding the therapeutic efficacy of TEO against the drug-resistant clinical isolates of C. albicans. The findings may be used in the development of a new antifungal agent accordingly.

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Section: Discussionsupporting

confidence: 92%

Susceptibility of Fluconazole-Resistant Candida albicans to Thyme Essential Oil

Alshaikh

Perveen

2021

Microorganisms

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“…Tan & Peterson (2005) and de Vasconcelos et al (2011) performed disc diffusion assays, which fail to determine susceptible dose-dependent isolates and often rely on a subjective interpretation. Linares et al (2003), Kirkpatrick et al (2010) and Patterson et al (1996a, b) added fluconazole or other antifungals to the CHROMagar, but could only identify highly sensitive and highly resistant isolates. These studies showed promise, but required use of multiple plates and, once again, failed to differentiate the susceptible dose-dependent susceptibility category.…”

Section: Introductionmentioning

confidence: 99%

X-Plate Technology: a new method for detecting fluconazole resistance in Candida species

Chadwick

Schuyler

Vermitsky

et al. 2013

Journal of Medical Microbiology

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Candida species are responsible for many opportunistic fungal infections. Fluconazole is a welltolerated antifungal drug, commonly used in the treatment of candidiasis. However, with fluconazole resistance ever increasing, rapid detection and antifungal susceptibility testing of Candida is imperative for proper patient treatment. This paper reports a cost-effective, simple and rapid chromogenic agar dilution method for simultaneous Candida species identification and fluconazole susceptibility testing. The results obtained by X-Plate Technology were in absolute concordance with standard microbroth dilution assays. Analysis of 1383 clinical patient samples with suspected vulvovaginal candidiasis revealed that this technology was able to detect and speciate the Candida isolate and determine the fluconazole susceptibility. The prevalence and susceptibility profiles of the clinical isolates using this method were highly similar to published reports using the microbroth dilution method.

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