CHROMagar Yersinia, a New Chromogenic Agar for Screening of Potentially Pathogenic Yersinia enterocolitica Isolates in Stools

Renaud, Nicolas; Lecci, Laetitia; Courcol, René; Simonet, Michel; Gaillot, Olivier

doi:10.1128/jcm.02903-12

Cited by 25 publications

(24 citation statements)

References 18 publications

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“…All of the Y. pseudotuberculosis strains (59/59) failed to grow on this medium with supplements ( Table 1). Renaud et al 9) reported that no Y. pseudotuberculosis strains grew on CAYe, and our result was the same. Among the 176 pathogenic Y. enterocolitica strains, 167 strains were able to grow on CIN agar, while 175 strains were able to grow on CAYe.…”

Section: Resultssupporting

confidence: 85%

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Evaluation of Chromogenic Medium for Selective Isolation of <i>Yersinia</i>

Thuan

Naher

Kubo

et al. 2016

Journal of the Food Hygienics Society of Japan

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Cefsulodin-irgasan-novobiocin agar (CIN) has been used as a selective agar to detect Yersinia in food or human patients; however, its components can inhibit the growth of some strains of Yersinia isolates grew on both media after 48 hr of incubation at 32 . Of the 10 pathogenic Y. enterocolitica O3 which could not grow on CIN or CAYe, 9 strains could not grow on CIN with supplements and 1 strain could not grow CAYe with supplements. Of 9 strains which did not grow on CIN with supplements, 3 strains could not grow on CIN without supplements. However, 1 strain which did not grow on CAYe with supplements could grow on CAYe without supplements. All of the Y. pseudotuberculosis strains could grow on CIN with/without supplements and on CAYe without supplements. The results indicate that the inhibition of the growth of Y. enterocolitica O3 on CIN is related to the components of CIN; however, the inhibition on CAYe appears to be related to the supplements in CAYe. Therefore, CAYe may be a more useful selective medium than CIN for pathogenic Y. enterocolitica .

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Section: Resultssupporting

confidence: 85%

“…Yersinia (CAYe), has been found to be useful for the identification of pathogenic Y. enterocolitica colonies 9) .…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Chromogenic Medium for Selective Isolation of <i>Yersinia</i>

Thuan

Naher

Kubo

et al. 2016

Journal of the Food Hygienics Society of Japan

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“…The value of chromogenic media has been demonstrated in a number of studies that reported improved sensitivity and specificity over traditional selective/differential media for the recovery of Salmonella, Shigella, STEC, Vibrio, and Yersinia (267)(268)(269)(270)(271). However, these media are more expensive than traditional media and are not available for in vitro diagnostic use for Vibrio and Yersinia in the United States at this time.…”

Section: Specimen Processingmentioning

confidence: 99%

Practical Guidance for Clinical Microbiology Laboratories: Diagnosis of Bacterial Gastroenteritis

2015

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SUMMARY Bacterial gastroenteritis is a disease that is pervasive in both the developing and developed worlds. While for the most part bacterial gastroenteritis is self-limiting, identification of an etiological agent by bacterial stool culture is required for the management of patients with severe or prolonged diarrhea, symptoms consistent with invasive disease, or a history that may predict a complicated course of disease. Importantly, characterization of bacterial enteropathogens from stool cultures in clinical laboratories is one of the primary means by which public health officials identify and track outbreaks of bacterial gastroenteritis. This article provides guidance for clinical microbiology laboratories that perform stool cultures. The general characteristics, epidemiology, and clinical manifestations of key bacterial enteropathogens are summarized. Information regarding optimal specimen collection, transport, and processing and current diagnostic tests and testing algorithms is provided. This article is an update of Cumitech 12A (P. H. Gilligan, J. M. Janda, M. A. Karmali, and J. M. Miller, Cumitech 12A, Laboratory diagnosis of bacterial diarrhea, 1992).

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“…CIN agar is the most frequently used agar for naturally contaminated samples because of the high confirmation rate of presumptive isolates and its relatively high selectivity (Laukkanen et al 2010;Savin et al 2012). Moreover, two selective chromogenic media have recently been designed for isolation of potentially pathogenic Y. enterocolitica strains (Weagant 2008;Renaud et al 2013). …”

Section: Detection Methodsmentioning

confidence: 99%

Enteropathogenic Yersinia spp.

Fredriksson-Ahomaa

2014

Zoonoses - Infections Affecting Humans and Animals

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Yersinia enterocolitica and Yersinia pseudotuberculosis can cause enteric yersiniosis among humans and animals. The infection is typically acquired through contaminated food but it can also be transmitted directly through human or animal contact. Common symptoms are diarrhoea, abdominal pain and fever but sometimes sequelae as joint pain and skin rash occur. The severity of the infection depends on the age and immunity of the infected person, the virulence of the strain and the infection dose. Y. enterocolitica and Y. pseudotuberculosis differ clearly from each other both pheno-and genotypically. Y. enterocolitica species consists of a very heterogeneous group of bacteria and not all Y. enterocolitica strains are pathogenic. Y. pseudotuberculosis strains show little variation in biochemical reactions and they are all considered to be pathogenic. Both plasmid and chromosomal encoded virulence factors are needed for Yersinia pathogenicity and all pathogenic strains carry a virulence plasmid which essential for the bacteria to multiply and disseminate in the host. Yersinia strains can grow at low temperatures and under both aerobic and anaerobic conditions but they are heat sensitive. The isolation and identification of enteropathogenic Yersinia is difficult and time consuming, especially when food and environmental samples are studied. More accurate methods should be designed for isolation and identification of Yersinia spp. and more information is needed about transmission routes and sources of enteropathogenic Yersinia strains.

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CHROMagar Yersinia, a New Chromogenic Agar for Screening of Potentially Pathogenic Yersinia enterocolitica Isolates in Stools

Cited by 25 publications

References 18 publications

Evaluation of Chromogenic Medium for Selective Isolation of <i>Yersinia</i>

Evaluation of Chromogenic Medium for Selective Isolation of <i>Yersinia</i>

Practical Guidance for Clinical Microbiology Laboratories: Diagnosis of Bacterial Gastroenteritis

Enteropathogenic Yersinia spp.

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