Chromate detoxification using combination of ChromeBac™ system and immobilized chromate reductase beads

Ishak, Amir Fuhaira; Karim, Norsuhada Abdul; Ahmad, Wan Azlina; Zakaria, Zainul Akmar

doi:10.1016/j.ibiod.2016.03.020

Cited by 15 publications

(7 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Human activities including electroplating and alloy production have had profound impacts on the discharge of chromium (Cr) into aquifers . Excessive intake of hexavalent chromate [Cr(VI)] (0.1 mg L −1 of MCL in drinking water proposed by U.S. EPA) can cause carcinogenic, teratogenic and mutagenic effects in animals and humans . Reduction of the most toxic and mobile Cr(VI) to less toxic and insoluble Cr(III) by denitrifying bacteria is a common approach to remove Cr(VI) from contaminated groundwater …”

Section: Introductionmentioning

confidence: 99%

“…9 Excessive intake of hexavalent chromate [Cr(VI)] (0.1 mg L −1 of MCL in drinking water proposed by U.S. EPA) can cause carcinogenic, teratogenic and mutagenic effects in animals and humans. 10,11 Reduction of the most toxic and mobile Cr(VI) to less toxic and insoluble Cr(III) by denitrifying bacteria is a common approach to remove Cr(VI) from contaminated groundwater. [12][13][14] Previous studies have demonstrated that synchronous reduction of NO 3 − and Cr by microorganisms can be achieved in a variety of bioreactors, involving the application of a cylindrical reactor with sulfur as the electron donor, 3 a granular sludge www.soci.org Y Hu et al…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Insights into simultaneous microbial chromium and nitrate reduction: inhibitory effects and molecular mechanisms

Chen

et al. 2019

J of Chemical Tech & Biotech

View full text Add to dashboard Cite

BACKGROUND: The potential effect of chromium on the denitrification process has drawn much attention recently. The efficient remediation of groundwater contaminated by both hexavalent chromate [Cr(VI)] and nitrate (NO 3 − ) was hampered due to the deficiency of denitrification in the presence of Cr(VI). The goal of this work was to understand the mechanisms of how Cr(VI) affected denitrification. RESULTS:To better understand the underlying nature, Cr(VI) effects on denitrification were investigated in batch. Negative effects of Cr(VI) exposure on denitrification were confirmed and ascribed to restriction of bacterial activities, by regulating functional genes expression and altering community composition. Stronger inhibition of the expression of denitrifying genes was observed with increased Cr(VI) loading (0-50 mg L −1 ). The critical inhibitory concentration and IC 50 of Cr(VI) on electron transport system activities were calculated to be 3.21 and 4.87 mg L −1 , respectively. Further amplicon analysis revealed that Betaproteobacteria were enriched, implying their potential key role in simultaneously removing nitrate and Cr(VI). The presence of Cr(VI) also upregulated the metabolic activities related to apoptosis. CONCLUSION: These findings shed light on the biogeochemical fates of Cr(VI) and NO 3− in aquifers, and may contribute in enhancing their practical application for bioremediation using microbial processes. ETSA assayElectron transport activity of the bacteria was measured by reducing 2-(p-iodophenyl)-3-(p-nitrophenyl)-5-phenyl tetrazolium wileyonlinelibrary.com/jctb

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Insights into simultaneous microbial chromium and nitrate reduction: inhibitory effects and molecular mechanisms

Chen

et al. 2019

J of Chemical Tech & Biotech

View full text Add to dashboard Cite

show abstract

“…Other researchers also reported on the evaluation of Cr(VI) reducing ability by environmental isolate either in batch [39,89] or continuous bacterial biofilm system [77,[90][91][92][93][94]. One example is the ability of a locally isolated gram negative aerobic Acinetobacter haemolyticus (A. haemolyticus) EF369508 that can carry out both Cr(VI) resistant and reducing properties [89].…”

Section: Volcanic Geothermal Regionmentioning

confidence: 99%

“…However, 16S rRNA identification for bacterial species present in the formed biofilm on the sugarcane bagasse (after more than 30 days of operation), did not reveal substantial presence of A. haemolyticus with Chitinophaga terrae, Laribacter hongkongensis, Ottowia thiooxydans, Rhizobium cellulosilyticum and Candidate division OP10 being the dominant species. Some important characteristics for the chromate reductase activities for A. haemolyticus have been reported [94] where results obtained are as follows: not NADH-dependent, associated with CFE with notable contribution from the membrane fraction, enhanced in the presence of glucose, optimal at pH 7.0, 30°C, in the presence of 1 mM Co 2+ (highest) with Michaelis-Menten constant, K m , and maximum reaction rate, V max , of 184.47 μM and 33.3 nmol/min/mg protein, respectively. The copresence of Ag + and Hg 2+ ions inhibited the enzyme activity.…”

Section: Volcanic Geothermal Regionmentioning

confidence: 99%

Bacterial Reduction of Cr(VI): Operational Challenges and Feasibility

et al. 2021

Self Cite

View full text Add to dashboard Cite

Purpose of Review Hexavalent chromium, Cr(VI), and trivalent chromium, Cr(III), are two chromium compounds with practical importance due to their high occurrence and solubility in the environment. Current Cr(VI) treatment techniques involve chemical reduction of Cr(VI) to Cr(III), which posed serious threat to workers and environment notably from long exposure and toxic fumes.Recent Findings Numerous reports have demonstrated the feasibility of using biological processes for the treatment of Cr(VI) industrial effluents by either pure culture or a consortium of Cr(VI)-reducing bacteria, with various degrees of success. Among issues to be considered include high cost of nutrient for the bacteria, low Cr resistant-reducing ability of environmental isolates, difficulty in scaling up finding in the laboratory to pilot scale and on-site application as well as the understanding on the dynamic underlying mechanisms for bacterial Cr(VI) reduction. Summary This review highlights cytotoxicity and genotoxicity properties of Cr(VI), which form the biggest motivation for continuous development in the field of Cr(VI) treatment technologies, latest finding in aerobic and anaerobic bacterial reduction of Cr(VI), operational challenges for bacterial Cr(VI) reduction, and some examples for laboratory-scale and pilot-scale evaluation of free and immobilized (biofilm) cells of Cr(VI) resistant-reducing bacteria.

show abstract

“…One of the main approaches applied to resolve these di culties is to immobilize biocatalyst into solid supports that can produce recoverable and stable heterogeneous biocatalysts, and is considered as a very powerful tool to improve biocatalyst stability and properties (Ishak et al 2016, Esmaeili and Farrahi 2016; Wu et al 2019). The use of immobilized microorganisms is superior to free cells in different applications that exhibited enhanced stability, facilitate its recovery and reuse (Kathiravan et al 2010;Lu et al 2018;Dey and Paul 2020).…”

Section: Introductionmentioning

confidence: 99%

Hexavalent chromium detoxification by haloalkaliphilic Nesterenkonia sp strain NRC-Y immobilized in different matrices

Maany,

Wahba,

Abo-Alkasem

et al. 2023

Biocatalysis and Biotransformation

View full text Add to dashboard Cite

In order to develop a bioprocess for Cr(VI) detoxi cation in industrial e uent, a previously isolated potent Cr(VI) reducing haloalkaliphilic Nesterenkonia sp strain NRC-Y was immobilized in nine different matrices including natural polymer, modi ed natural, synthetic and mixtures of natural and synthetic polymers and investigated for Cr(VI) detoxi cation. Among the tested carriers and immobilization approaches, Nesterenkonia sp NRC-Y cells encapsulated in amidated pectin beads showed highest Cr(VI) reduction e ciency (58.4% of initial Cr(VI) concentration 150 mg/L after 20 h), followed by alginate, alginate-PVA, alginate-PVA-chitosan, PVA-PVP, and PVA respectively. Therefore, amidated pectin was selected for further investigation and immobilization of both whole cells and chromate reductase.Operational stability study revealed that immobilized whole cell was more e cient and stable than immobilized chromate reductase and the free cells retaining about 60%, 27.0% and 11.5% of its initial activity after four successive batches, respectively. The temperature and pH optima for the immobilized cells were 35 C and 7.0, respectively. The pH and thermal stability of Nesterenkonia sp NRC-Y cells were signi cantly enhanced upon immobilization in amidated pectin beads. The developed immobilized biocatalyst was applied for Cr(VI) reduction in industrial e uent samples, and was able to completely reduce Cr(VI) within 4 and 8 h for e uents for initial Cr(VI) concentrations of 10 and 30 mg/L, respectively. To the best of our knowledge, this is the rst report about of Cr(VI) detoxi cation by immobilized Nesterenkonia sp NRC-Y in amidated pectin beads. The developed immobilized biocatalyst is promising and has the potential for large-scale Cr(VI) detoxi cation application.

show abstract

Chromate detoxification using combination of ChromeBac™ system and immobilized chromate reductase beads

Cited by 15 publications

References 29 publications

Insights into simultaneous microbial chromium and nitrate reduction: inhibitory effects and molecular mechanisms

Insights into simultaneous microbial chromium and nitrate reduction: inhibitory effects and molecular mechanisms

Bacterial Reduction of Cr(VI): Operational Challenges and Feasibility

Hexavalent chromium detoxification by haloalkaliphilic Nesterenkonia sp strain NRC-Y immobilized in different matrices

Contact Info

Product

Resources

About