Chromate tolerance and removal of bacterial strains isolated from uncontaminated and chromium-polluted environments

Tamindžija, Dragana; Chromiková, Zuzana; Spaić, Andrea; Barák, Imrich; Bernier‐Latmani, Rizlan; Radnović, Dragan

doi:10.1007/s11274-019-2638-5

Cited by 15 publications

(10 citation statements)

References 78 publications

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“…Some studies found that the culturable number of various microbial groups in the contaminated soil samples is decreased, compared to a number in uncontaminated samples [16]. Contrary, other studies did not find a statistically significant correlation between the metal concentration in soil (Cr) and cultivable bacteria count nor with the percentage of chromate tolerant cultivable bacteria [17,18]. The functional diversity and composition of the microbial community can be also assessed by using the Biolog-Microplate method [19], restriction fragment length polymorphism (RFLP) based on 16S rRNA sequence of microbes [20,21] as well as the PCR-DGGE analysis [22].…”

Section: Interaction Of Heavy Metals With Microorganismsmentioning

confidence: 93%

“…The speciation of any metal in nature depends on the combined effects of pH, redox potential, and ionic strength. Bioavailability of metals is a complex phenomenon, influenced by many physical, chemical, and biological factors such as pH, eH, ion exchange capacity, organic matter content, clay content, presence of ligands, metal concentration, and speciation [16,17]. Some heavy metals are essential for the normal function of a microbial cell, such as iron and copper contained in cytochrome c oxidase [13], and manganese, which is important for oxygen production during photosynthesis [14].…”

Section: Interaction Of Heavy Metals With Microorganismsmentioning

confidence: 99%

“…As). Many species, such as Bacillus cereus, Bacillus subtilis, Pseudomonas aeruginosa, Pseudomonas fluorescens, and Escherichia coli, are capable of reducing highly toxic hexavalent chromium to its biologically inert, less toxic trivalent form [8,17,[33][34][35][36][37][38]. Besides chromium, it is reported that bacteria can reduce mercury [39], iron [40], arsenic [41], uranium [42], manganese [43], molybdenum [44], and other metals, and oxidize As [45,46].…”

Section: Interaction Of Heavy Metals With Microorganismsmentioning

confidence: 99%

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Microbial bioremediation of heavy metals

Volarić

Svirčev

Tamindžija

et al. 2021

Hem Ind

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Heavy metal pollution is one of the most serious environmental problems, due to metal ions persistence, bioavailability, and toxicity. There are many conventional physical and chemical techniques traditionally used for environmental clean-up. Due to several drawbacks regarding these methods, the use of living organisms, or bioremediation, is becoming more prevalent. Biotechnological application of microorganisms is already successfully implemented and is in constant development, with many microbial strains successfully removing heavy metals. This paper provides an overview of the main heavy metal characteristics and describes the interactions with microorganisms. Key heavy metal resistance mechanisms in microorganisms are described, as well as the main principles and types of heavy metal bioremediation methods, with details on successful pilot scale bioreactor studies. Special attention should be given to indigenous bacteria isolated from the polluted environments since such species are already adapted to contamination and possess resistance mechanisms. Utilization of bacterial biofilms or consortia could be advantageous due to higher resistance and a combination of several metabolic pathways, and thus, the possibility to remove several heavy metals simultaneously. Novel technologies covered in this review, such as nanotechnology, genetic engineering, and metagenomics, are being introduced to the field of bioremediation in order to improve the process. To conclude, bioremediation is a potentially powerful solution for cleaning the environment.

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Section: Interaction Of Heavy Metals With Microorganismsmentioning

confidence: 93%

Section: Interaction Of Heavy Metals With Microorganismsmentioning

confidence: 99%

Section: Interaction Of Heavy Metals With Microorganismsmentioning

confidence: 99%

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Microbial bioremediation of heavy metals

Volarić

Svirčev

Tamindžija

et al. 2021

Hem Ind

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Section: Introductionmentioning

confidence: 99%

“…In a previous study, we isolated a highly Cr-resistant environmental strain, Bacillus pseudomycoides NCr1a ( Tamindžija et al, 2019 ). This strain exhibited resistance to 8 mM Cr(VI) in minimal M9 medium and, thus, was far more resistant to Cr(VI) than any of the other environmental strains isolated in that study ( Tamindžija et al, 2019 ). Here, using scanning transmission electron microscopy-energy dispersive spectroscopy (STEM-EDS), we mapped and quantified chromium accumulation by individual cells of the Cr-resistant environmental strain and compared it to that of the Cr-tolerant, but not resistant B. subtilis PY79.…”

Section: Introductionmentioning

confidence: 99%

Implantation of Bacillus pseudomycoides Chromate Transporter Increases Chromate Tolerance in Bacillus subtilis

et al. 2022

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Chromium of anthropogenic origin contaminates the environment worldwide. The toxicity of chromium, a group I human carcinogen, is greatest when it is in a hexavalent oxidation state, Cr(VI). Cr(VI) is actively transported into the cell, triggering oxidative damage intracellularly. Due to the abundance of unspecific intracellular reductants, any microbial species is capable of bio-transformation of toxic Cr(VI) to innocuous Cr(III), however, this process is often lethal. Only some bacterial species are capable of sustaining the vegetative growth in the presence of a high concentration of Cr(VI) and thus operate as self-sustainable bioremediation agents. One of the successful microbial Cr(VI) detoxification strategies is the activation of chromate efflux pumps. This work describes transplantation of the chromate efflux pump from the potentially pathogenic but highly Cr resistant Bacillus pseudomycoides environmental strain into non-pathogenic but only transiently Cr tolerant Bacillus subtilis strain. In our study, we compared the two Bacillus spp. strains harboring evolutionarily diverged chromate efflux proteins. We have found that individual cells of the Cr-resistant B. pseudomycoides environmental strain accumulate less Cr than the cells of B. subtilis strain. Further, we found that survival of the B. subtilis strain during the Cr stress can be increased by the introduction of the chromate transporter from the Cr resistant environmental strain into its genome. Additionally, the expression of B. pseudomycoides chromate transporter ChrA in B. subtilis seems to be activated by the presence of chromate, hinting at versatility of Cr-efflux proteins. This study outlines the future direction for increasing the Cr-tolerance of non-pathogenic species and safe bioremediation using soil bacteria.

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Heavy Metals: Source, Toxicity Mechanisms, Health Effects, Nanotoxicology and Their Bioremediation

Siddiqa

Faisal

2020

Contaminants in Agriculture

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Chromate tolerance and removal of bacterial strains isolated from uncontaminated and chromium-polluted environments

Cited by 15 publications

References 78 publications

Microbial bioremediation of heavy metals

Microbial bioremediation of heavy metals

Implantation of Bacillus pseudomycoides Chromate Transporter Increases Chromate Tolerance in Bacillus subtilis

Heavy Metals: Source, Toxicity Mechanisms, Health Effects, Nanotoxicology and Their Bioremediation

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