Chromatic polymer assays for the analysis of lipid and lipoprotein peroxidation

Abstract

Evaluation of the extent of lipoprotein oxidation and the effects of therapeutic and dietary agents upon lipid peroxidation processes are critical in understanding and combating atherosclerotic diseases. Bioanalytical chromatic assays based upon polydiacetylene (PDA) have recently emerged as a useful platform for analysis of lipoprotein oxidation. This article describes the principles of PDA-based sensing, and its application to the study of lipids peroxidation, both in purified lipoprotein samples, as well as in blood plasma specimens.

A Colorimetric Strip for Rapid Detection and Real-Time Monitoring of Histamine in Fish Based on Self-Assembled Polydiacetylene Vesicles

“…Friedman et al argued that the surface charge of protein can be analyzed by the change of DMPC/PDA film from blue to red, laying a solid foundation for the application of PDA in the field of biosensor . After that the sensing methods based on PDA for detecting proteins, viruses, enzymes, and polysaccharides have been constantly established. − Even so, when it comes to biochemical detection, it is inevitable to link specific recognition probes, which requires complex modification methods and is time-consuming. Besides, far too little attention has been paid to the application of PDA combined with an intelligent strip to detect and monitor food spoilage.…”

Polydiacetylene Supramolecules: Synthesis, Characterization, and Emerging Applications

“…In their analysis of a PDA film for the sensing of membrane-active compounds, Kolusheva, Jelinek, and colleagues developed an approach that quantifies the RGB values of scanned images . This quantification approach has gained recent popularity especially for analyzing fabricated PDA films. − ,− Volinksy et al derived this digital colorimetric analysis (DCA) method from Pratt, in which the red chromaticity level, r , is defined as …”