2016
DOI: 10.1016/bs.mcb.2016.04.019
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Chromatin immunoprecipitation and an open chromatin assay in zebrafish erythrocytes

Abstract: Zebrafish is an excellent genetic and developmental model for the study of vertebrate development and disease. Its ability to produce an abundance of transparent, externally developed embryos has facilitated large-scale genetic and chemical screens for the identification of critical genes and chemical factors that modulate developmental pathways. These studies can have profound implications for the diagnosis and treatment of a variety of human diseases. Recent advancements in molecular and genomic studies have… Show more

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Cited by 8 publications
(15 citation statements)
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“…To investigate whether Gata1 regulates Hemogen in zebrafish, we analyzed a Gata1 ChIP-seq dataset that was generated to assess Gata1 activity in adult zebrafish erythrocytes (Yang et al, 2016). Fig.…”
Section: Resultsmentioning
confidence: 99%
“…To investigate whether Gata1 regulates Hemogen in zebrafish, we analyzed a Gata1 ChIP-seq dataset that was generated to assess Gata1 activity in adult zebrafish erythrocytes (Yang et al, 2016). Fig.…”
Section: Resultsmentioning
confidence: 99%
“…Figure 5A shows that Gata1 bound to CNE1 and CNE2 at sites overlapping their Gata motifs (red lines), which indicates strongly that Gata1 is required for transcription of Hemogen in zebrafish. Corroboration that CNE1 and CNE2 were active chromatin regions was provided by ATAC-seq and DNase I hypersensitive site analysis (Yang et al, 2016) (Fig. 5A).…”
Section: Hematopoietic and Neural Expression Of Hemogen In Zebrafish mentioning
confidence: 99%
“…In mammals, transcription of Hemogen from the proximal promoter is tightly regulated by Gata1 in hematopoietic cells (Yang et al, 2006). To investigate whether Gata1 regulates Hemogen in zebrafish, we analyzed a Gata1 ChIP-seq dataset that was generated to assess Gata1 activity in adult zebrafish erythrocytes (Yang et al, 2016). Figure 5A shows that Gata1 bound to CNE1 and CNE2 at sites overlapping their Gata motifs (red lines), which indicates strongly that Gata1 is required for transcription of Hemogen in zebrafish.…”
Section: Hematopoietic and Neural Expression Of Hemogen In Zebrafish mentioning
confidence: 99%
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