Chromatin meets the cytoskeleton: the importance of nuclear actin dynamics and associated motors for genome stability

Wollscheid, Hans-Peter; Ulrich, Helle D.

doi:10.1016/j.dnarep.2023.103571

Cited by 9 publications

(3 citation statements)

References 104 publications

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“…Since, as a transcriptional regulator, slug is mainly present in the cell nucleus, we then focused on the nuclear compartment. We [22] and others have previously demonstrated the importance of nuclear actin to the repair of DNA damage [7,9,26]. In good accordance with previous results [22], we validated the synergistic effect of manipulating the actin dynamics via small molecules and treatment with either a DNA-damaging compound (doxorubicin) or UV irradiation.…”

Section: Discussionsupporting

confidence: 90%

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A Novel Interaction of Slug (SNAI2) and Nuclear Actin

Zhuo,

Stöckl,

Fröhlich

et al. 2024

Cells

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Actin is a protein of central importance to many cellular functions. Its localization and activity are regulated by interactions with a high number of actin-binding proteins. In a yeast two-hybrid (Y2H) screening system, snail family transcriptional repressor 2 (SNAI2 or slug) was identified as a yet unknown potential actin-binding protein. We validated this interaction using immunoprecipitation and analyzed the functional relation between slug and actin. Since both proteins have been reported to be involved in DNA double-strand break (DSB) repair, we focused on their interaction during this process after treatment with doxorubicin or UV irradiation. Confocal microscopy elicits that the overexpression of actin fused to an NLS stabilizes complexes of slug and γH2AX, an early marker of DNA damage repair.

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Section: Discussionsupporting

confidence: 90%

“…Research from recent years has revealed that actin and several actin-binding proteins also exist in the nucleus [6,7]. Therefore, actin has been shown to participate in transcriptional regulation, as well as in DNA damage repair [7][8][9][10].…”

Section: Introductionmentioning

confidence: 99%

A Novel Interaction of Slug (SNAI2) and Nuclear Actin

Zhuo,

Stöckl,

Fröhlich

et al. 2024

Cells

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“…Actin interacts with essentially all transcribed genes in Drosophila ovaries [ 47 ], copurifies with all three eukaryotic RNA polymerases [ 44 , 45 , 48 ], and regulates the activity of specific transcription factors [ 49 ]. In addition to gene expression, actin is linked to DNA replication [ 50 ], DNA damage response [ 51 , 52 , 53 , 54 , 55 ], and long-range chromatin motion [ 56 , 57 , 58 ].…”

Section: Other Cytoskeleton Proteins Also Localize and Function In Th...mentioning

confidence: 99%

Non-Canonical Localization of Cardiac Troponins: Expanding Functions or Causing Pathologies?

Arifulin,

Sheval

2024

IJMS

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The troponin complex—consisting of three subunits: troponin C (TnC), cardiac troponin I (cTnI) and cardiac troponin T (cTnT)—plays a key role in the regulation of myocardial contraction. Troponins are preferentially localized in the cytoplasm and bind to myofibrils. However, numerous, albeit scattered, studies have shown the presence of troponins in the nuclei of muscle cells. There is increasing evidence that the nuclear localization of troponins may be functionally important, making troponins an important nuclear player in the pathogenesis of various diseases including cancer and myopathies. Further studies in this area could potentially lead to the development of treatments for certain pathologies. In this review, we collected and discussed recent data on the properties of non-canonically localized cardiac troponins, the molecular mechanisms leading to this non-canonical localization, and the possible functions or pathological effects of these non-canonically localized troponins.

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