Chromatin Preparation and Chromatin Immuno-precipitation from Drosophila Embryos

Löser, Eva; Latreille, Daniel; Iovino, Nicola

doi:10.1007/978-1-4939-6380-5_3

Cited by 3 publications

(1 citation statement)

References 6 publications

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“…The considerable gain in recovery afforded by NChIP means that very small amounts of tissue can be analyzed (21), allowing for the study of hPTM profiles in a single tissue of individual insects. For example, NChIP has been used to study cis-regulatory evolution among lepidopterans (76), as well as to study Drosophila tissues (83).…”

Section: Assaying Dna-associated Proteinsmentioning

confidence: 99%

Epigenetics in Insects: Genome Regulation and the Generation of Phenotypic Diversity

Glastad

Hunt

Goodisman

2019

Annu. Rev. Entomol.

133

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Epigenetic inheritance is fundamentally important to cellular differentiation and developmental plasticity. In this review, we provide an introduction to the field of molecular epigenetics in insects. Epigenetic information is passed across cell divisions through the methylation of DNA, the modification of histone proteins, and the activity of noncoding RNAs. Much of our knowledge of insect epigenetics has been gleaned from a few model species. However, more studies of epigenetic information in traditionally nonmodel taxa will help advance our understanding of the developmental and evolutionary significance of epigenetic inheritance in insects. To this end, we also provide a brief overview of techniques for profiling and perturbing individual facets of the epigenome. Doing so in diverse cellular, developmental, and taxonomic contexts will collectively help shed new light on how genome regulation results in the generation of diversity in insect form and function.

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Section: Assaying Dna-associated Proteinsmentioning

confidence: 99%

Epigenetics in Insects: Genome Regulation and the Generation of Phenotypic Diversity

Glastad

Hunt

Goodisman

2019

Annu. Rev. Entomol.

133

View full text Add to dashboard Cite

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Analyzing the Genome-Wide Distribution of Histone Marks by CUT&Tag in Drosophila Embryos

Zenk

Cardamone

Morales

et al. 2023

Methods in Molecular Biology

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Do social insects support Haig's kin theory for the evolution of genomic imprinting?

et al. 2017

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Although numerous imprinted genes have been described in several lineages, the phenomenon of genomic imprinting presents a peculiar evolutionary problem. Several hypotheses have been proposed to explain gene imprinting, the most supported being Haig's kinship theory. This theory explains the observed pattern of imprinting and the resulting phenotypes as a competition for resources between related individuals, but despite its relevance it has not been independently tested. Haig's theory predicts that gene imprinting should be present in eusocial insects in many social scenarios. These lineages are therefore ideal for testing both the theory's predictions and the mechanism of gene imprinting. Here we review the behavioral evidence of genomic imprinting in eusocial insects, the evidence of a mechanism for genomic imprinting and finally we evaluate recent results showing parent of origin allele specific expression in honeybees in the light of Haig's theory.

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Chromatin Preparation and Chromatin Immuno-precipitation from Drosophila Embryos

Cited by 3 publications

References 6 publications

Epigenetics in Insects: Genome Regulation and the Generation of Phenotypic Diversity

Epigenetics in Insects: Genome Regulation and the Generation of Phenotypic Diversity

Analyzing the Genome-Wide Distribution of Histone Marks by CUT&Tag in Drosophila Embryos

Do social insects support Haig's kin theory for the evolution of genomic imprinting?

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