“…Downstream of BMP4, BME-treated hPSCs showed increased pSMAD1/5/9, leading to upregulation of GATA3, TFAP2A, CDX2, and indirectly of EOMES. EOMES is essential for hPGCLC formation (Kobayashi et al ., 2017; Kojima et al ., 2017), but its continued and high expression has been shown to promote differentiation to endoderm (Heslop et al ., 2022; Yoney et al ., 2022). Consistent with this, EOMES is moderately expressed in D2-progenitor cells exposed to the BME overlay.…”