Chromatin signal in genome size measurements

Vinogradov, Alexander E.

doi:10.1002/(sici)1097-0320(19991101)37:3<243::aid-cyto12>3.0.co;2-k

Cited by 2 publications

(1 citation statement)

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“…These observations support the notion that the use of DNA‐specific fluorochromes with a known base pair preferential binding (e.g., DAPI, which has a preferential binding to AT‐rich DNA sequences) may provide significantly different DNA cell content values, for the same species, compared with the use of DNA intercalating dyes (38, 39). This is particularly relevant because nucleated cells from a different animal species, which may display significant differences in their relative DNA A‐T base pair contents, are used for reference (38, 39). In the present study, we systematically used human diploid blood leukocytes as reference nuclei.…”

Section: Discussionsupporting

confidence: 77%

Flow cytometry measurement of the DNA contents of G0/G1 diploid cells from three different teleost fish species

et al. 2002

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Background:Although there is a lot information in the literature about genome size in fish, a high variability among data for the same species is reported, being mainly related to methodological aspects. Flow cytometry-based fluorescence measurements of intercalating dyes is the most attractive approach due to its precision, objectivity, high speed, and relative simplicity. Methods: We analyze the DNA content of G0/G1 diploid nuclei of three teleost species (Carassius auratus, Tinca tinca, and Danio rerio) using flow cytometry. Forty-three animals were used and up to 50,000 retinal cells were analyzed per sample. Propidium iodide-associated fluorescence was assessed using a FACSCalibur flow cytometer. Standard human leukocytes were used as a reference.

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Section: Discussionsupporting

confidence: 77%

Flow cytometry measurement of the DNA contents of G0/G1 diploid cells from three different teleost fish species

et al. 2002

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Automated identification of diploid reference cells in cervical smears using image analysis

Laak¹,

Siebers²,

Cuijpers³

et al. 2002

Cytometry

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Linear and cyclic analogues of cyclolinopeptide A (CLA) with two dipeptide segments (Val5Pro6 and Pro6Pro7) replaced by their tetrazole derivatives were synthesized by the SPPS technique and cyclized using TBTU (O‐(benzotriazol‐1‐yl)‐1,1,3,3‐tetramethyluronium tetrafluoroborate) reagent. The conformational properties of the c(Leu1Ile2Ile3Leu4Val5Pro6 ψ[CN4]Ala7Phe8Phe9) were investigated by NMR and computational techniques. The overall solution structure of this cyclic peptide resembles that observed for the CLA in the solid state. These studies of cyclic tetrazole CLA analogue confirm that the 1,5‐disubstituted tetrazole ring functions as an effective, well‐tolerated cis‐amide bond mimic in solution. The peptides were examined for their immunosuppressive activity in the humoral response test. For cyclic analogues the immunosuppressive activity, at low doses, is equal in magnitude to the activity presented by cyclosporin A and native CLA. The conformational and biological data seem indicate that the ProProPhePhe moiety and the preservation of the CLA backbone conformation are important for immunosuppressive activity. © 2002 Wiley Periodicals, Inc. Biopolymers 63: 343–357, 2002

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Chromatin signal in genome size measurements

Cited by 2 publications

References 10 publications

Flow cytometry measurement of the DNA contents of G0/G1 diploid cells from three different teleost fish species

Flow cytometry measurement of the DNA contents of G0/G1 diploid cells from three different teleost fish species

Automated identification of diploid reference cells in cervical smears using image analysis

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