Chromatin states of core pluripotency-associated genes in pluripotent, multipotent and differentiated cells

Barrand, Sanna; Collas, Philippe

doi:10.1016/j.bbrc.2009.11.134

Cited by 32 publications

(25 citation statements)

References 27 publications

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“…As reported in previous studies on human stem cells (Ulloa-Montoya et al 2007;Yalvac et al 2010), Oct-4, Nanog and Sox-2 showed different relative gene expression in the cell line isolated and this is probably due to different regulatory mechanisms of these genes (Barrand and Collas 2010). Amnion cells did not express CD34, a known cell marker expressed by hematopoietic stem cells.…”

Section: Discussionmentioning

confidence: 46%

Isolation and differentiation potential of an equine amnion-derived stromal cell line

et al. 2011

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Stem cells represent an important tool in veterinary therapeutic field such as tissue engineering. In the present study, equine amnion-derived mesenchymal stromal cells were investigated for applications in veterinary science as an alternative source to bone marrow mesenchymal stem cells and adipose stem cells. Amnion stromal cells isolation and characterization protocol is described; the in vitro cell growth rate was calculated by measuring viable cell number over 20 days. The expression of stem cell markers such as Oct-4, Nanog, Sox-2 and CD105 was assessed by retrotranscription quantitative PCR (RT-qPCR) and differentiation into adipocytes, osteocytes and chondrocytes precursors was analyzed by cytochemical staining. This study showed that amnion stromal cells expressing stem cell markers can differentiate into mesoderm lineage and may be an alternative source to mesenchymal stem cells derived from adipose tissue and bone marrow for the use in tissue repair.

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Section: Discussionmentioning

confidence: 46%

Isolation and differentiation potential of an equine amnion-derived stromal cell line

et al. 2011

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“…Numerous studies have found that the capacity of proliferation and differentiation of adult stem cells decreased with age and degeneration, which may result from lessened expression of genes regulating the maintenance of stemness of stem cells (34)(35)(36)(37). Furthermore, expression of stemness genes has an important role in the regulation of multilineage differentiation of stem cells and decreases with cell differentiation (38)(39)(40). Tsai et al (41) also found that Oct4 and Nanog genes were highly expressed in adult MSCs, and that proliferation and differentiation of MSCs decreased after knockout of the Oct4 and Nanog genes.…”

Section: Discussionmentioning

confidence: 99%

Comparison of biological characteristics of nucleus pulposus mesenchymal stem cells derived from non-degenerative and degenerative human nucleus pulposus

Jia

Yang

et al. 2017

Experimental and Therapeutic Medicine

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Abstract. Cell therapy using mesenchymal stem cells provides a promising approach for the treatment of intervertebral disc degeneration (IDD). In recent years, human nucleus pulposus mesenchymal stem cells (NPMSCs) have been identified in nucleus pulposus tissue and displayed great potential for the regeneration of IDD. However, biological differences between non-degenerative and degenerative nucleus pulposus-derived NPMSCs have remained to be defined. The aim of the present study was to compare the biological characteristics of human NPMSCs derived from non-degenerative and degenerative nucleus pulposus. NPMSCs were isolated from non-degenerative and degenerative nucleus pulposus, which were assessed using the Pfirrmann grading system. The biological characteristics of the NPMSCs, including the expression of surface markers, multipotent differentiation, colony formation, chemotactic cell migration, cell activity and stemness gene expression were compared. It was found that NPMSCs could be obtained from non-degenerative and degenerative human nucleus pulposus. However, degenerative nucleus pulposus-derived NPMSCs displayed decreased ability of colony formation, chemotactic migration, cell activity and expression of stemness genes compared with non-degenerative nucleus pulposus-derived NPMSCs. Therefore, NPMSCs derived from non-degenerative and degenerative nucleus pulposus show different biological behaviors. The degenerative status of nucleus pulposus tissue should be considered when selecting NPMSCs as a source for clinical application.

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“…Despite the considerable information about silencing of pluripotency ES genes during differentiation, very little is known about the epigenetic control of genes associated with self-renewal and maintenance of multipotent adult stem cells. In adipose-derived stem cells (ASCs) and mesenchymal stem cells from bone marrow (BM-MSCs), OCT4 is silenced by promoter hypermethylation, whereas Nanog and Sox2 are unmethylated despite the repressed state of the genes [19]. The same patterns of methylation were found in differentiated fibroblasts and keratinocytes [19].…”

Section: Dna Methylation-dependent Regulation Of Genes Associated Witmentioning

confidence: 99%

“…In adipose-derived stem cells (ASCs) and mesenchymal stem cells from bone marrow (BM-MSCs), OCT4 is silenced by promoter hypermethylation, whereas Nanog and Sox2 are unmethylated despite the repressed state of the genes [19]. The same patterns of methylation were found in differentiated fibroblasts and keratinocytes [19]. It seems that, whereas Oct4 regulation is strongly influenced by CpG promoter hypermethylation, the control of Nanog and Sox2 expression could be due to other repressive mechanisms such as histone modification patterns [19].…”

Section: Dna Methylation-dependent Regulation Of Genes Associated Witmentioning

confidence: 99%

DNA methylation in stem cell renewal and multipotency

Berdasco

Esteller

2011

Stem Cell Res Ther

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Owing to their potential for differentiation into multiple cell types, multipotent stem cells extracted from many adult tissues are an attractive stem cell resource for the replacement of damaged tissues in regenerative medicine. The requirements for cellular differentiation of an adult stem cell are a loss of proliferation potential and a gain of cell-type identity. These processes could be restricted by epigenetic modifications that prevent the risks of lineage-unrelated gene expression or the undifferentiated features of stem cells in adult somatic cells. In this review, we focus on the role of DNA methylation in controlling the transcriptional activity of genes important for self-renewal, the dynamism of CpG methylation of tissue-specific genes during several differentiation programs, and whether the multilineage potential of adult stem cells could be imposed early in the original precursor stem cells through CpG methylation. Additionally, we draw attention to the role of DNA methylation in adult stem cell differentiation by reviewing the reports on spontaneous differentiation after treatment with demethylating agents and by considering the evidence provided by reprogramming of somatic cells into undifferentiated cells (that is, somatic nuclear transfer or generation of induced pluripotent cells). It is clear from the evidence that DNA methylation is necessary for controlling stem cell proliferation and differentiation, but their exact contribution in each lineage program is still unclear. As a consequence, in a clinical setting, caution should be exerted before employing adult stem cells or their derivatives in regenerative medicine and appropriate tests should be applied to ensure the integrity of the genome and epigenome.

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Chromatin states of core pluripotency-associated genes in pluripotent, multipotent and differentiated cells

Cited by 32 publications

References 27 publications

Isolation and differentiation potential of an equine amnion-derived stromal cell line

Isolation and differentiation potential of an equine amnion-derived stromal cell line

Comparison of biological characteristics of nucleus pulposus mesenchymal stem cells derived from non-degenerative and degenerative human nucleus pulposus

DNA methylation in stem cell renewal and multipotency

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