Chromatin structure and the state of human organism

Shckorbatov, Y. G.; Zhuravlyova, Lyubov A.; Navrotskaya, Valeriya; Miroshnichenko, Elena V.; Montvid, Pavel Y.; Shakhbazov, V.G.; Sutushev, Takhir A.

doi:10.1016/j.cellbi.2004.11.015

Cited by 6 publications

(6 citation statements)

References 8 publications

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“…Although many specific regulatory programs involve targeted alterations in chromatin structure (e.g., Sirtuins and the dietary restriction response) heterochromatin granules in human buccal epithelium showed a circadian rhythm with greater condensation at night and less in morning (following antioxidant recharging?). Physical activity caused condensation, likely mediated by catecholamines and cortisol [245].…”

Section: The Mammalian Regulatory Bauplan: It's About Timementioning

confidence: 99%

Dopamine and Aging: Intersecting Facets

Rollo

2008

Neurochem Res

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Aging encompasses life itself so understanding requires frameworks that forge unity amidst complexity. The free radical theory of aging is one example. The original focus on damage was augmented recently by appreciation that reactive oxygen and nitrogen species are essential to normal signaling and cell function. This paradigm is currently undergoing an explosive expansion fueled by the discovery that regulatory organization is a merry-go-round of redox cycling seamlessly fused to endogenous clocks. This might best be described as an "Electroplasmic Cycle." This is certainly applicable to dopaminergic neurons with their exceptional metabolic, electrical and rhythmic properties. Here I review normal aging of dopamine systems to highlight them as a valuable model. I then examine the possible integration of free radical and ion channel theories of aging. Finally, I incorporate clocks and explore the multifaceted implications of electroplasmic cycles with special emphasis on dopamine.

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Section: The Mammalian Regulatory Bauplan: It's About Timementioning

confidence: 99%

Dopamine and Aging: Intersecting Facets

Rollo

2008

Neurochem Res

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“…Highly significant presence of cysteine suggests its role as a critical residue for SVMPs activity and thus these SVMPs may be investigated for possible role in diseased conditions. Further analysis of the amino acid composition can help to place amino acid presence at remarkable level and be correlated with precise pathological conditions (Shckorbatov et al, 2008). Other physico-chemical parameters also signify the behavior of SVMPs in different conditions (Table 3).…”

Section: Resultsmentioning

confidence: 99%

Identification of accelerated evolution in the metalloproteinase domain of snake venom metalloproteinase sequences (SVMPs) through comparative analysis

Islam¹,

Roly²,

Ruhullah³

et al. 2016

Afr. J. Biotechnol.

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Computational protein sequence analysis is one of the most important tools used for understanding the evolution of closely related proteins sequences including snake venom metalloproteinase sequences (SVMPs) which give valuable information regarding genetic variations. The fundamental objective of the present study is to screen the evolution distributed in metalloproteinase domain regions of protein sequences among different SVMPs in snake species which are involved in a range of pathological disorders such as arthritis, atherosclerosis, liver fibrosis, cardiovascular, cancer, liver and neurodegenerative disorders. In fact, SVMPS are responsible for hemorrhage and may also interfere with the hemostatic system. A comparative characterization of the metalloproteinase sequences has been carried out to analyze their multiple sequence alignment, phylogenic tree, homology, physicochemical, secondary structural and functional properties. DNAMAN software was used for multiple sequence alignment, phylogenic tree and homology and Expasy's Prot-param server was used for amino acid composition, physico-chemical and functional characterization of these SVMPs sequences. Studies of secondary structure of these SVMPs were carried out by computational program. Based on the observed patterns of occurrence of atypical features, we hypothesize that amino acids of metalloproteinase domain region (66.63% identity) of protein sequences are highly changeable; whereas, signal peptide region (93.98% identity) is the lowest changeable protein sequence and the remaining other three domains such as propeptide region (87.36% identity), desintegrin domain region (78.63% identity) and cysteine-rich domain region (75.70% identity) show moderate changeable protein sequence. SVMPs might be an accelerated evolution, which is a key player in causing diseases. From the data, it can be suggested that over -changed metalloproteinase domain regions in snake venom metalloproteinase might be responsible for the generation of functional variation of proteins expressed, which in turn may lead to different disorders in humans after snake bite. The results of this study would be an effective tool for the study of mutation, drugs resistance mechanisms and development of new drugs for different diseases.

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“…In the first case the buccal epithelium cells obtained from the donor were uniformly spread over the glass. In the second case the cell samples were stained with 2 % orcein solution in 45 % acetic acid [2].…”

Section: Problem Statementmentioning

confidence: 99%

“…The investigation on the cellular level of the mechanisms of damaging factors influence may elucidate the mechanisms of stress factors influence on the whole organism [2]. For this reason, developments of new methods of the investigation of the functional state of cells are very urgent.…”

Section: Introductionmentioning

confidence: 99%