Chromatographic analysis (LC-MS and GC-MS), antioxidant activity, antibacterial activity, total phenol, and total flavonoid determination of
            <i>Cleome arabica</i>
            L. growing in Jordan

Alkhatib, Rami; Almasarweh, Afnan B.; Abdo, Nour; Mayyas, Abdulraouf; Al–Qudah, Mahmoud A.; Abu‐Orabi, Sultan T.

doi:10.1080/10942912.2022.2115063

Cited by 11 publications

(13 citation statements)

References 31 publications

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“…The presence of bioactive compounds in the different parts of C. felina contributes to the therapeutic values of the plants in the treatment of diseases. In contrast to this, previously, several Cleome species were studied for the existence of phytochemical compounds in C. spinosa leaves and root extracts of cyclohexane, chloroform, ethyl acetate, and methanolic solvents [22]; C. rutidosperma leaves to water and ethanolic extracts [23], C. gynandra methanolic leaves extract [24], and C. arabica whole-plant aqueous-methanol, butanol, and water extracts [25], and it was reported the presence of flavonoids, saponins, terpenoids, anthraquinones, cardiac glycosides, tannins, carbohydrates, amino acids, proteins, polyphenols, reducing sugars, and alkaloids. The early preliminary findings reported by Silva et al [22] and Alkhatib et al [25] have determined that among comparative analyses between the different solvent extracts, the methanolic extract exhibited stronger positivity towards active phytochemical constitution than the other selective solvents during their studies.…”

Section: Discussionmentioning

confidence: 99%

Biological Characterization of Cleome felina L.f. Extracts for Phytochemical, Antimicrobial, and Hepatoprotective Activities in Wister Albino Rats

Shaikh,

Niazi,

Bepari

et al. 2023

Antibiotics

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The present study aims to explore the phytochemical constitution and biological activities of Cleome felina L.f. (Cleomaceae). C. felina (leaves, stem, and root) extracts (acetone, methanol, and water) were qualitatively assessed for phytochemical presence. Methanolic leaves extract revealed more positive phyto-compounds among all the extracts; further, methanolic leaves extract was evaluated for FTIR, EDX, GCMS, antimicrobial assay, acute toxicity, and paracetamol-induced hepatoprotective activity in Wister albino rats. FTIR and EDX analysis unveiled important functional groups and elements in the leaves. GCMS analysis of methanolic leaves extract exposed 12 active phyto-compounds: major constituents detected were 1-Butanol, 3-methyl-, formate-48.79%; 1-Decanol, 2-ethyl-13.40%; 1,6-Anhydro-β-d-talopyranose-12.49%; Ethene, 1,2-bis(methylthio)-7.22%; Decane-4.02%; 3-Methylene-7, 11-dimethyl-1-dodecene-3.085%; Amlexanox-2.50%; 1,2,3,4-Cyclopentanetetrol, (1α,2β,3β,4α)-2.07%; L-Cysteine S-sulfate-1.84%; n-Hexadecanoic acid-1.70%; and Flucarbazone-1.55%. The antimicrobial assay showed a moderate zone of inhibition against S. aureus, B. cereus, E. coli, P. aeruginosa, C. albicans, and C. glabrata at 100 µL/mL concentration. Additionally, acute toxicity revealed no behavioral sign of the toxic effect. The significant results were obtained for methanolic leaves extract (low-50 and high-100 mg/kg b.wt. dose) for hepatoprotective activity, where it dramatically reduced serum blood biochemical markers (AST, ALT, ALP, Total bilirubin, and cholesterol) and exhibited elevated hepatic antioxidant enzymes (SOD, CAT, and GSH) concentration with lipid peroxidation retardation. To conclude, C. felina methanolic leaves extract ameliorated important phytochemical compounds and showed significant antimicrobial and hepatoprotective efficacy; therefore, utilization of C. felina leaves suggested in pharmacological applications, and in numerous cosmetics, herbicides, and food industries, would be a great scope for future hepatoprotective drug designing.

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Section: Discussionmentioning

confidence: 99%

Biological Characterization of Cleome felina L.f. Extracts for Phytochemical, Antimicrobial, and Hepatoprotective Activities in Wister Albino Rats

Shaikh,

Niazi,

Bepari

et al. 2023

Antibiotics

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“…In the current investigation, essential oil was extracted from the fresh aerial parts of S. peyronii , as described previously in the literature [ 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 ]. Aerial parts of S. peyronii (including stem, leaves, and flowers) were chopped into small pieces and then hydro-distillation was carried out in a Clevenger-type apparatus for 4 h. The oil was then dried over anhydrous Na 2 SO 4 and kept in GC-grade n -hexane at 4 °C until the analysis was performed.…”

Section: Methodsmentioning

confidence: 99%

“…The antioxidant activities of the crude extracts and essential oils were determined by the DPPH and ABTS radical-scavenging methods according to the procedure described in the literature [ 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 ]. The scavenging activity was then measured according to the following equation:

where A C is the absorbance of the blank and A S is the absorbance in the presence of the extract.…”

Section: Methodsmentioning

confidence: 99%

Scrophularia peyronii Post. from Jordan: Chemical Composition of Essential Oil and Phytochemical Profiling of Crude Extracts and Their In Vitro Antioxidant Activity

Al-Dalahmeh,

Almahmoud,

Al-Bataineh

et al. 2023

Life

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The genus Scrophularia is one of the largest genera belonging to the Scrophulariaceae family. Different members of the genus exhibit an interesting, wide spectrum of bioactivities. Accordingly, the current study aimed to investigate, for the first time, the chemical composition of the essential oil of Scrophularia peyronii Post. from Jordan. Additionally, extracts obtained from the aerial parts with solvents of different polarities were assayed for their phytochemical constituents and in vitro antioxidant activities. The major constituents detected in the essential oil, as revealed by GC/MS analysis, contained mainly Z,Z-farnesyl acetone (11.04%), β-elemene (6.36%), n-octanal (5.98%), and spathulenol (4.58%). Each of the aqueous methanol (Sp-M) and butanol (Sp-B) extracts contained flavonoids, saponins, anthraquinone, and glycosides. Both extracts were evaluated for their total phenolic content (TPC), total flavonoid content (TFC), and their in vitro antioxidant activity, which were assayed using the DPPH radical scavenging activity and ABTS radical scavenging methods. Additionally, the two extracts were then subjected to LC-ESI-MS/MS for the qualitative determination of their secondary metabolite content, especially in flavonoids and phenolic compounds. The results showed that the Sp-B extract of S. peyronii had the highest contents of both phenolic compounds and flavonoids and showed high radical scavenging activity, as determined by the two assay methods, when compared with the Sp-M extract. The LC-ESI-MS/MS analysis resulted in the detection of 21 compounds, including 8 flavonoids, 6 phenolic acids, 6 iridoids, and 2 acids. Although the majority of compounds were detected in both extracts, it was noticed that scropolioside B, 6′-O-cinnamoylharpagide, isoferulic acid, and 6-O-methylcatapol were only detected in the Sp-M fraction.

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Section: Introductionmentioning

confidence: 99%

“…3 A large number of studies have reported that natural flavonoids are effective antioxidants, most of which possess the ability to scavenge free radicals and prevent oxidative stress-related diseases. [4][5][6] As typical phenolic antioxidants, natural flavonoids include various structural types, such as flavones, isoflavones, flavonols, and flavanones. They usually have 4′,5,7-OHs as antioxidant sites and scavenge free radicals through hydrogen atom transfer (HAT), single electron transfer followed by proton transfer (SET-PT), and sequential proton loss electron transfer (SPLET) mechanisms.…”

Section: Introductionmentioning

confidence: 99%

Antioxidant mechanisms and products of four 4′,5,7-trihydroxyflavonoids with different structural types

Chen

et al. 2023

RSC Med. Chem.

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Chromatographic analysis (LC-MS and GC-MS), antioxidant activity, antibacterial activity, total phenol, and total flavonoid determination of Cleome arabica L. growing in Jordan

Cited by 11 publications

References 31 publications

Biological Characterization of Cleome felina L.f. Extracts for Phytochemical, Antimicrobial, and Hepatoprotective Activities in Wister Albino Rats

Biological Characterization of Cleome felina L.f. Extracts for Phytochemical, Antimicrobial, and Hepatoprotective Activities in Wister Albino Rats

Scrophularia peyronii Post. from Jordan: Chemical Composition of Essential Oil and Phytochemical Profiling of Crude Extracts and Their In Vitro Antioxidant Activity

Antioxidant mechanisms and products of four 4′,5,7-trihydroxyflavonoids with different structural types

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