Chromatographic analysis of bromhexine and oxytetracycline residues in milk as a drug analysis medium with greenness profile appraisal

Sharkawi, Marco M Z; Safwat, Mark T.; Abdelaleem, Eglal A.; Abdelwahab, Nada S.

doi:10.1039/d2ay01462a

Cited by 7 publications

(2 citation statements)

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“…As a result, many greenness evaluation tools have been developed for this purpose, such as the analytical eco‐scale (ESA) (Gałuszka et al, 2012), the analytical greenness metric approach (AGREE) (Pena‐Pereira et al, 2020) and the green analytical procedure index (GAPI) (Płotka‐Wasylka, 2018). Using more than one evaluation tool may give a complete picture of the greenness profiles of the method, as each metric method gives particular information regarding certain issues of the developed method (Sharkawi et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

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A validated ecofriendly chromatographic method for determination of myasthenia gravis combined medications in spiked human plasma

Magdy

Farid

Anwar

et al. 2023

Biomedical Chromatography

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Recently, the main interest of analytical chemistry researchers has been the development of green analytical methods to minimize harmful effects on the environment and natural life. Therefore, an RP‐HPLC method was developed and assessed regarding its greenness criteria using three greenness assessment tools: an analytical eco‐scale, an analytical greenness metric approach and a green analytical procedure index. This method aims to separate and quantitatively determine three co‐administered drugs, namely pyridostigmine bromide (PYR), 6‐mercaptopurine (MRC) and prednisolone (PRD), in their tertiary mixture and spiked human plasma. These drugs are co‐administered to manage myasthenia gravis autoimmune disease. The separation was done using a C18 column and a gradient elution of a mixture of 0.1% H3PO4 aqueous solution (pH 2.3) and methanol. The flow rate was adjusted to 1 ml/min and detection was done at 254 (for PYR and PRD) and at 330 nm (for MRC). The lower limits of quantitation were 15, 2, and 5 μg/ml for PYR, MER and PRD, respectively. Linear correlations were obtained and found to be near 1. In addition, the proposed method was validated according to the US Food and Drug Administration's instructions, and the results proved its success to determine the three studied drugs in their tertiary mixture and spiked human plasma.

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Section: Introductionmentioning

confidence: 99%

“…(AGREE) (Pena-Pereira et al, 2020) and the green analytical procedure index (GAPI) (Płotka-Wasylka, 2018). Using more than one evaluation tool may give a complete picture of the greenness profiles of the method, as each metric method gives particular information regarding certain issues of the developed method (Sharkawi et al, 2022).…”

mentioning

confidence: 99%