Chromatographic analysis of phospholipids

“…The adsorption of glucoamylase on the amylose 383 complexed with LPL is very low (Kitahara et al, 1996). Since it appears that enzyme 384 resistance of the amylose-lipid complex could be utilised to manipulate the GI for a range 385 of rice food products ( addition of antioxidants such as BHT (Christie, 1985). 448…”

Section: Phospholipids and Rice Storage 285mentioning

confidence: 99%

“…These include (1) gradients of hexane-isopropanol-water 527 (Demandre et al, 1985), (2) isocratic elution with acetonitrile-methanol-sulphuric acid 528 (Christie, 1985) and (3) and so more in vivo human study is needed (Kullenberg et al, 2012). 560…”

Section: Column Chromatography Tlc and Gc Analysis Of Pls 472mentioning

confidence: 99%

Phospholipids in rice: Significance in grain quality and health benefits: A review

et al. 2013

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“…PC and PE fractions of the extract were separated by normal phase HPLC equipped with a fraction collector using a Kromasil column (12.5 ! 0.46 cm, Si 5 m, 100 nm, Chromatographie Service, Langerwehe, Germany) and an elution system consisting of n -hexane/isopropanol (80: 20, v/v) and methanol [22] . After evaporation of the elution solvents using a centrifugal evaporator, PC and PE were methylated with trimethylsulfonium hydroxide [23] .…”

Section: Lipid Analysismentioning

confidence: 99%

Methionine-Induced Elevation of Plasma Homocysteine Concentration Is Associated with an Increase of Plasma Cholesterol in Adult Rats

et al. 2006

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Background and Aims: Dietary methionine affects cholesterol metabolism in growing rats. Methionine effects on adult rats and mechanisms by which methionine alters the lipid metabolism are not fully elucidated. We investigated possible mechanisms by which dietary methionine acts on lipid metabolism of adult rats. Methods: Male adult rats were divided into three groups (n = 10) and were fed casein-based diets differing in methionine concentration (low-methionine diet: 0.96 g/kg; adequate-methionine diet: 2.22 g/kg, high-methionine diet: 6.82 g/kg) for 4 weeks. Concentrations of triacylglycerols and cholesterol in plasma and lipoproteins, concentration of homocysteine in plasma, concentration of cholesterol in liver, fecal lipid excretion, expression of hepatic HMG-CoA reductase, phosphatidylethanolamine N-methyltransferase 2 (PEMT-2) and of LDL receptor were measured. Results: Rats fed the high-methionine diet had higher plasma homocysteine concentrations than rats fed the low-methionine diet (p < 0.05). Although concentrations of cholesterol in plasma and lipoproteins were not different between the groups, there was a distinct positive correlation between circulating plasma homocysteine and plasma cholesterol (R² = 0.55, p < 0.001). The fecal excretion of cholesterol and bile acids was not altered by dietary methionine. The relative mRNA concentration of HMG-CoA reductase and of LDL receptor remained unaffected by dietary methionine. Gene expression of PEMT-2 was higher in rats fed the high-methionine diet than in rats fed the other diets (p < 0.05). Conclusion: The results demonstrate that dietary methionine contributes to a rise in circulating homocysteine concentration which positively correlates with the concentration of plasma cholesterol. However, the effects of methionine on cholesterol metabolism of adult rats were relatively weak.

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Chromatographic analysis of phospholipids

Cited by 33 publications

References 139 publications

Properties, analysis and purification of milk polar lipids

Properties, analysis and purification of milk polar lipids

Phospholipids in rice: Significance in grain quality and health benefits: A review

Methionine-Induced Elevation of Plasma Homocysteine Concentration Is Associated with an Increase of Plasma Cholesterol in Adult Rats

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