Chromatographic analysis of phytochemicals in Costus igneus and computational studies of flavonoids

Peasari, John Reddy; Motamarry, Sneha sri; Varma, Karthikeya Srinivasa; Anitha, P.; Potti, Ravindra Babu

doi:10.1016/j.imu.2018.10.004

Cited by 17 publications

(9 citation statements)

References 19 publications

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“…41 ; Test for Phytosterol in the leaf extracts of CI using Libermann Burchard's (++) and Salkowski's Test (+) depicted the presence of phytosterols in the leaf samples of CI 43 ; Test for Flavonoids in the leaf extracts of CI using Ferric Chloride Test (++) and Shinod's Test (++) showed the presence of flavonoids as indicated by Reddy et al 44 ; Test for Fixed Oils and Fats in the leaf extracts of CI Spot Test (+); ; Saponification (+); ; Test for Free Amino Acids in the leaf extracts of CI Million's Reagent (++); Ninhydrin Reagent (++); Test for Tannins in the leaf extracts of CI using 5% Ferric Chloride (-) and 10% Lead Acetate (-) showed no results indicating the absence of tannins in the sample; Test for Saponins in the leaf extracts of CI Foam Test (+) indicated the presence of saponins; however, gums & mucilage were absent in CIMLE samples (Table 1). In the present study, phytochemical screening of methanolic leaf extracts of C. igneus showed the presence of alkaloids, glycosides, saponins, phytosterols, phenolics, and carbohydrates as indicated earlier by Khanday et al 45 . 46,47 (Table 2; Fig.…”

Section: Fourier Transform Infrared Spectroscopy (Ftir) Analysissupporting

confidence: 82%

Phytochemical Screening, GC-MS and FTIR analysis of Bioactive Compounds in Methanolic Leaf Extracts of Costus igneus (CIMLE) as a Natural Source of Drug Lead Molecules for Next-generation Drug-design, Development and Therapeutics

Vijaya

Selvi²,

John³

et al. 2022

J. Drug Delivery Ther.

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Diabetes mellitus (DM) is a metabolic disorder that affects people of all ages. Increased prevalence of diabetes worldwide has led to the development of synthetic drugs to provide an interim solution to the ill effects of the issue. However, such drugs, although effective as antihyperglycemic agents, are accompanied by significant side effects, costly, and inaccessible to majority of people in remote in underdeveloped countries. Local medicinal plants have been used by such indigenous people through ages to treat such complicate ailments due to their ease of availability and GRAS nature. As diabetes continues to become prevalent, health care practitioners are considering PBNPs as a potential source of antidiabetic drugs due to their high potency and fewer side effects. To better understand the mechanism of action of medicinal plants, their active phytoconstituents are being isolated and investigated thoroughly. Phytochemical screening followed by GCMS analysis of methanolic leaf extracts of Costus igneus depicted the presence of 19 bioactive compounds viz., Bicyclo [3.1.1]heptane, 2,6,6- trimethyl-; 2-Pentadecanone, 6,10,14-trimethyl-; phthalic acid; 3,7,11,15-Tetramethyl-2-hexadecen- 1-ol; 5,9,13-Pentadecatrien-2-one, 6,10,14- trimethyl; 2(3H)-Furanone,dihydro-5-methyl-5- pentyl; Hexamethyl-2,6,10,14,18,22- tetracosahexaen-1-yl]-6-methox.; Oxalic acid, cyclohexyl isohexyl ester; 6-Octadecenoic acid; Phytol; 4,8,12,16-Tetramethylheptadecan-4- olide; 5-methyl-2,3-dihydro-1H-indole; Bis(2-ethylhexyl) phthalate; Adamantane-1-carboxylic acid methyl ester; Benzopyran-6-ol,3,4-dihydro-; Benzoquinoline; gamma-Tocopherol; dimethyl-5,5'-diphenyl-1H,; 1H-benzo[b]furo[2,3-f]indole that may be ADMET predicted for potential biomedical application in the treatment of diabetics and long term complications associated with the disease. Keywords: Costus igneus; CIMLE; GCMS; Phytochemical Screening; Antidiabetic Leads; NDDT; Antidiabetic Medicinal Plant

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Section: Fourier Transform Infrared Spectroscopy (Ftir) Analysissupporting

confidence: 82%

Phytochemical Screening, GC-MS and FTIR analysis of Bioactive Compounds in Methanolic Leaf Extracts of Costus igneus (CIMLE) as a Natural Source of Drug Lead Molecules for Next-generation Drug-design, Development and Therapeutics

Vijaya

Selvi²,

John³

et al. 2022

J. Drug Delivery Ther.

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“…Among them, there are many groups of secondary metabolites, including alkaloids, saccharides, terpenoids, glycosides, steroids, tannins, saponins, phenols, flavonoids, etc. [ 140 , 141 , 142 , 143 ]. Besides the extract of this herb also contains trace elements such as K, Ca, Cr, Mn, Cu, and Zn [ 144 ].…”

Section: Bioactive Compounds From Plants Having Type 2 Antidiabetimentioning

confidence: 99%

Bioactive Compounds in Anti-Diabetic Plants: From Herbal Medicine to Modern Drug Discovery

Tran

Bao

2020

Biology

292

113

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Natural products, including organisms (plants, animals, or microorganisms) have been shown to possess health benefits for animals and humans. According to the estimation of the World Health Organization, in developing countries, 80% of the population has still depended on traditional medicines or folk medicines which are mostly prepared from the plant for prevention or treatment diseases. Traditional medicine from plant extracts has proved to be more affordable, clinically effective and relatively less adverse effects than modern drugs. Literature shows that the attention on the application of phytochemical constituents of medicinal plants in the pharmaceutical industry has increased significantly. Plant-derived secondary metabolites are small molecules or macromolecules biosynthesized in plants including steroids, alkaloids, phenolic, lignans, carbohydrates and glycosides, etc. that possess a diversity of biological properties beneficial to humans, such as their antiallergic, anticancer, antimicrobial, anti-inflammatory, antidiabetic and antioxidant activities Diabetes mellitus is a chronic disease result of metabolic disorders in pancreas β-cells that have hyperglycemia. Hyperglycemia can be caused by a deficiency of insulin production by pancreatic (Type 1 diabetes mellitus) or insufficiency of insulin production in the face of insulin resistance (Type 2 diabetes mellitus). The current medications of diabetes mellitus focus on controlling and lowering blood glucose levels in the vessel to a normal level. However, most modern drugs have many side effects causing some serious medical problems during a period of treating. Therefore, traditional medicines have been used for a long time and play an important role as alternative medicines. Moreover, during the past few years, some of the new bioactive drugs isolated from plants showed antidiabetic activity with more efficacy than oral hypoglycemic agents used in clinical therapy. Traditional medicine performed a good clinical practice and is showing a bright future in the therapy of diabetes mellitus. World Health Organization has pointed out this prevention of diabetes and its complications is not only a major challenge for the future, but essential if health for all is to be attained. Therefore, this paper briefly reviews active compounds, and pharmacological effects of some popular plants which have been widely used in diabetic treatment. Morphological data from V-herb database of each species was also included for plant identification.

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“…The molecules in structure data format were downloaded from Pubchem using their structure CIDs. The geometry of the molecules was optimized using the OPLS2005 force field and low energy conformers generated in the LigPrep module of Schrodinger (Peasari et al, 2018). The generated conformers were then used in molecular docking studies.…”

Section: Ligand Preparationmentioning

confidence: 99%

HMG‐CoA reductase inhibition medicated hypocholesterolemic and antiatherosclerotic potential of phytoconstituents of an aqueous pod extract of Prosopis cineraria (L.) Druce: In silico, in vitro, and in vivo studies

Jaipal

Ram

Charan

et al. 2022

eFood

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Hydrophilic bioactive compounds are copiously exhibited in aqueous extracts owed to solubility. The study was assigned to assess the ability of phytoconstituents of aqueous pod extract of Prosopis cineraria to inhibit 3‐hydroxy‐3‐methylglutary‐coenzyme A (HMG‐CoA) reductase activity and regression in atherosclerotic plaque through in vitro, in vivo, and in silico assessments along with phytochemistry of extract. The test extract exhibited 17 leading compounds as examined by Liquid Chromatograph Triple Quadrupole Mass Spectroscope. In vitro assay of test extract showed 78.1% inhibition of HMG‐CoA inhibition (IC50 was 0.03 μg/ml). In vivo assessments, hypercholesterolemia was induced by supplementing cholesterol powder and a high‐fat diet. The treatment of test extract caused significant (p ≤ 0.001) improvements in the lipid profile and antioxidant levels. Subsequently, the reductions in the atherosclerotic plaque and improved lumen volume were pointedly observed. In silico analyses of molecular docking revealed potent interaction capabilities of cloprostenol with the target protein of HMGR. The interactions were validated through structural simulations of the molecular dynamics such as root mean square fluctuation, the radius of gyration, and solvent accessible surface area. The druggability of potent compounds was also examined. The results revealed that phytoconstituents of the test extract could inhibit HMGR and regress atherosclerotic plaque.

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Chromatographic analysis of phytochemicals in Costus igneus and computational studies of flavonoids

Cited by 17 publications

References 19 publications

Phytochemical Screening, GC-MS and FTIR analysis of Bioactive Compounds in Methanolic Leaf Extracts of Costus igneus (CIMLE) as a Natural Source of Drug Lead Molecules for Next-generation Drug-design, Development and Therapeutics

Phytochemical Screening, GC-MS and FTIR analysis of Bioactive Compounds in Methanolic Leaf Extracts of Costus igneus (CIMLE) as a Natural Source of Drug Lead Molecules for Next-generation Drug-design, Development and Therapeutics

Bioactive Compounds in Anti-Diabetic Plants: From Herbal Medicine to Modern Drug Discovery

HMG‐CoA reductase inhibition medicated hypocholesterolemic and antiatherosclerotic potential of phytoconstituents of an aqueous pod extract of Prosopis cineraria (L.) Druce: In silico, in vitro, and in vivo studies

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