Chromatographic and colorimetric detection of glycosylated hemoglobins: A comparative analysis of two different methods

The colorimetric determination of HbAic in normal and diabetic subjects

“…In two recent pape some were subjected to critical comparison (Abraham et al 1978, Saibene et 6 I 979). Since most of the various methods have drawbacks, such as high investnier cost for the ones involving high pressure liquid chromatography or low efficienc for the column chromatography ones, it appeared a worthwhile task to standardiz the method originally alluded to in previous work (Fluckiger & Winterhalter 197;Fliickiger & Winterhalter 1978) and used for the comparative evaluation (Abrahan et al 1978, Saibene et al 1979. The presently described modified method has highe OD443 yields better reproducibility and correlates well with both the lsolab deter mination of fast hemoglobins and fasting blood sugar levels.…”

Glycated Haemoglobin: Comparison between Methods Based upon 5-Hydroxymethylfurfural Determination (Colorimetric or HPLC) and Ion Exchange Chromatography (HbA1)

“…In the colorimetric technique, 5-hydroxymethylfurfural is generally measured spectrophotometrically after reaction with thiobarbituric acid (13). Many authors (14)(15)(16)(17) have reported that this procedure is non-stoichiometric and requires careful standardization of assay conditions, including the concentration of total haemoglobin in the assayed aliquot, in order to achieve precision and permit comparison of results. In order to obtain more reliable results some authors (18 -20) reported the automatization of the colorimetric procedure.…”

Potent Antidiabetic Activity of Aqueous Extract of Brown Seaweed Padina Boergesenii in Streptozotocininduced Diabetic Rats

“…The protein, urea, uric acid and creatinine were assayed according to the methods of Lowry et al, 1951, [14] Natelson et al, 1951, [15] Caraway et al, 1963 [16] and Brod et al, 1964 [17] respectively. Estimation of hemoglobin andglycosylated hemoglobin were estimated according to the standard methods (Saibene et al, 1979 andDrabkin et al, 1932).…”

“…Estimation of hemoglobin andglycosylated hemoglobin were estimated according to the standard methods (Saibene et al, 1979 andDrabkin et al, 1932). [18,19] The lipid profile oftotal cholesterol, free cholesterol, phospholipids, free fatty acids and triglycerides were calculated by the standard methods of Parekh and Jung, 1970, [20] Rouser et al, 1970, [21] Horn and Mehanan, 1981 [22] and Rice, 1970 [23] respectively.…”