Chromatographic and electrophoretic strategies for the chiral separation and quantification of d‐ and l‐<i>threo</i> methylphenidate in biological matrices

Allen, Serena; Pond, Brooks B.

doi:10.1002/bmc.3312

Cited by 6 publications

(2 citation statements)

References 60 publications

(177 reference statements)

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“…Comparing our method with other approaches, it has to be kept in mind that the LLOQ of MPH strongly depends on the type and amount of the biological sample matrix. The published results varied between 0.006 and 30 ng/ml . Leis et al found an LLOQ of MPH in human plasma of 0.006 ng/ml, which they determined using a GC‐negative chemical ionization (NCI)‐MS combination.…”

Section: Resultsmentioning

confidence: 99%

Fast quantitative determination of methylphenidate levels in rat plasma and brain ex vivo by MALDI‐MS/MS

et al. 2015

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This study presents a simple and sensitive high-throughput matrix-assisted laser desorption/ionization time-of-flight tandem mass spectrometry (MALDI-MS/MS) method for ex vivo quantification of methylphenidate (MPH) in rat plasma and brain. The common MALDI matrix alpha-cyano-4-hydroxycinnamic acid was used to obtain an optimal dried droplet preparation. For method validation, standards diluted in plasma and brain homogenate prepared from untreated (control) rats were used. MPH was quantified within a concentration range of 0.1-40 ng/ml in plasma and 0.4-40 ng/ml in brain homogenate with an excellent linearity (R ≥ 0.9997) and good precision. The intra-day and inter-day accuracies fulfilled the FDA's ±15/20 critera. The recovery of MPH ranged from 93.8 to 98.5% and 87.2 to 99.8% in plasma and homogenate, respectively. We show that MPH is successfully quantified in plasma and brain homogenate of rats pre-treated with this drug using the internal standard calibration method. By means of this method, a linear correlation between plasma and brain concentration of MPH in rodents pre-treated with MPH was detected. The simple sample preparation based on liquid-liquid extraction and MALDI-MS/MS measurement requires approximately 10 s per sample, and this significantly reduces analysis time compared with other analytical methods. To the best of our knowledge, this is the first MALDI-MS/MS method for quantification of MPH in rat plasma and brain. Copyright © 2015 John Wiley & Sons, Ltd.

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Section: Resultsmentioning

confidence: 99%

Fast quantitative determination of methylphenidate levels in rat plasma and brain ex vivo by MALDI‐MS/MS

et al. 2015

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“…Allen et al depicted some important analytical techniques used for the determination of racemic mixtures of MPH. CE was one of the techniques used for chiral separation and detection of the drug in biological matrices [76]. CE was also used to study chiral drugs and their metabolites present in biological samples with sensitive detection and elucidation of their enantiomers and enantioselective actions [77][78][79].…”

Section: Electrophoresismentioning

confidence: 99%

Review on analytical methods for quantification of ADHD drugs in human biological samples

Sundari¹,

Amuthalakshmi²,

Nalini³

2020

Reviews in Analytical Chemistry

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Attention deficit hyperactivity disorder (ADHD) is a common neuro-developmental disorder. The symptoms of ADHD include difficulty in attention, memory and impulse control. Many pharmaceutical formulations (stimulants and non-stimulants) are available on the market to treat ADHD symptoms. The most commonly used drugs for treatment are amphetamine, methylphenidate, atomoxetine, bupropion, guanfacine and clonidine. In the field of pharmaceuticals, bioanalysis is an important tool used for the quantification of drugs and their metabolites present in biological samples using various analytical methods. Although a number of analytical methods were reported for the quantification of these drugs in biological samples of experimental animals, due to species differences, it is important to develop analytical methods to quantify these drugs in human biological samples to aid forensic and pharmacokinetic studies. In this review, we compile the bio-analytical methods such as spectrophotometry, spectrofluorimetry, mass spectrometry, electrophoresis, liquid chromatography and gas chromatography used for the quantification of ADHD drugs in human biological samples such as blood, plasma, serum, oral fluids, sweat, hair and urine based on earlier published articles from various journals.

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Enantioseparation performance of superficially porous particle vancomycin-based chiral stationary phases in supercritical fluid chromatography and high performance liquid chromatography; applicability for psychoactive substances

Folprechtová

Kalíková

Kadkhodaei

et al. 2021

Journal of Chromatography A

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Chromatographic and electrophoretic strategies for the chiral separation and quantification of d‐ and l‐threo methylphenidate in biological matrices

Cited by 6 publications

References 60 publications

Fast quantitative determination of methylphenidate levels in rat plasma and brain ex vivo by MALDI‐MS/MS

Fast quantitative determination of methylphenidate levels in rat plasma and brain ex vivo by MALDI‐MS/MS

Review on analytical methods for quantification of ADHD drugs in human biological samples

Enantioseparation performance of superficially porous particle vancomycin-based chiral stationary phases in supercritical fluid chromatography and high performance liquid chromatography; applicability for psychoactive substances

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