Chromatographic and mass spectral characterization of budesonide and a series of structurally related corticosteroids using LC–MS

Quantitative measurements of corticosteroids in ex vivo samples using on-line SPE-LC/MS/MS

“…These types of MS/MS signal interferences generated from isomeric cortisol compounds in biological samples when detecting cortisol have not been reported previously. In some of earlier LC/MS/MS studies, cortisol and cortisone were not completely separated in the HPLC [12,14,22,24]. It is therefore possible that the cortisol concentrations reported in some of the previous studies may have artificially augmented by the isomeric compounds.…”

“…LC/MS/MS methods have been reported for corticosteroids analysis [12][13][14][15][16] using both positive and negative electrospray ionization (ESI) tandem mass spectrometry (MS/MS) monitoring for in vitro and in vivo samples [15,[17][18][19][20]. It is generally believed that MS/MS is specific enough to distinguish compounds of interest from the interferences from the biological samples, so chromatography with partially overlapping peaks were often used [12,16,21,22].…”

Advances in the analysis of steroid hormone drugs in pharmaceuticals and environmental samples (2004–2010)

“…Further studies, elucidating the structures of impurities in budesonide [47,48], sprironolactone [49,50], megestrol acetate [51], flurogestone acetate [52], dutasteride [53] and degradation products in mometasone furoate [54] using methodological approach as outlined above are also worth mentioning.…”

Using total error concept for the validation of a liquid chromatography–tandem mass spectrometry method for the determination of budesonide epimers in human plasma

“…The analysis of budesonide in bulk drugs and pharmaceutical products is well described in the literature [5][6][7][8][9] and it is mentioned either in the European [10] or the United States Pharmacopoeias [11]. The determination of budesonide in biological fluids for pharmacokinetics studies has also been reported.…”