Chromatographic behavior of epirubicin and its analogues on high-purity silica in hydrophilic interaction chromatography

Li, Ruiping; Huang, Jiangen

doi:10.1016/j.chroma.2004.04.033

Cited by 92 publications

(39 citation statements)

References 26 publications

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“…3,4,5,6,7,8,9 The chromatographic selectivity for compounds using alcohol-based mobile phases differs significantly from that observed with acetonitrile. Also, due to the enhanced polarity of alcohols compared to acetonitrile, the overall retention decreases as well.…”

Section: 2mentioning

confidence: 83%

“…Also, due to the enhanced polarity of alcohols compared to acetonitrile, the overall retention decreases as well. 6,7 Nevertheless, the use of methanol, ethanol or isopropyl alcohol offers incontestable advantages such as enhanced solubility of many analytes and buffers as well as a lowered cost and production of less hazardous waste. 3,4,10 Enhanced-fluidity liquid chromatography (EFLC) is a method in which a significant proportion of a miscible gas is dissolved in the mobile phase.…”

Section: 2mentioning

confidence: 99%

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Characterization of enhanced-fluidity liquid hydrophilic interaction chromatography for the separation of nucleosides and nucleotides

Philibert

Olesik

2011

Journal of Chromatography A

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Hydrophilic Interaction Chromatography (HILIC) is a liquid chromatographic separation mechanism commonly used for polar biological molecules. While nucleotides are very polar analytes, only a few studies have been conducted on their separation using HILIC. Herein, the use of enhanced-fluidity liquid chromatography (EFLC) for the separation of nucleosides and nucleotides under HILIC conditions is investigated. Enhanced-fluidity liquid chromatography involves using common mobile phases with the addition of substantial proportions of a dissolved gas which provides enhanced mobile phase diffusivity and lower viscosity. The impact of varying mobile phase composition: buffer composition, type of base, salt used, salt concentration and mole fraction of CO 2 was studied to provide optimized HILIC separations. Each of these parameters plays a key role in the retention of the analytes, which demonstrates the complexity of the retention mechanism in HILIC. The tailing of phosphorylated compounds was overcome with the use of phosphate buffer and the addition of a strong base; efficiency and peak asymmetry were compared with the addition of either triethylamine (TEA), 1,4-diazabicyclo

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Section: 2mentioning

confidence: 83%

Section: 2mentioning

confidence: 99%

Characterization of enhanced-fluidity liquid hydrophilic interaction chromatography for the separation of nucleosides and nucleotides

Philibert

Olesik

2011

Journal of Chromatography A

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“…The different temperature effects are the result of multiple retention mechanisms. In addition to hydrophilic and ion-exchange interactions, solvent-solute and solventstationary phase hydrogen bondings are also involved [22]. The combination of these interactions resulted in different temperature effects from that in the ACN mobile phase.…”

Section: Resultsmentioning

confidence: 99%

Effect of Temperature on the Chromatographic Behavior of Epirubicin and its Analogues on High Purity Silica Using Reversed-Phase Solvents

Dong

Huang

2007

Chroma

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The influence of temperature on the retention behavior of epirubicin and its analogues on high purity silica with reversed-phase solvents has been systematically investigated. It was found that temperature effects on retention are highly dependent on the type and concentration of organic modifier, as well as the pH of the mobile phase. In organic-rich mobile phases, the type of organic modifier plays an important role. With an aprotic solvent as modifier, retention times show anomalous increases with elevated temperature. At the same time, both efficiency and resolution are significantly improved but this is not the situation with a protic solvent as modifier. In addition, temperature shows different effects on retention time and selectivity when the pH is changed, and temperature-dependent selectivity reversal is found at higher pHs. In aqueousrich mobile phases, regardless of the nature of the organic solvent and pH, retention of solutes drops as temperature is raised. It seems that the effect of temperature on chromatographic behavior of the solutes on bare silica using mobile phases containing various organic modifiers or pHs, results from a number of different retention mechanisms.

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“…45,47,56,[60][61][62][63] Studies by Li and Huang 62 showed better separation when MeCN was used as the mobile phase compared to MeOH, THF and isopropanol. Attempts by Dinh et al 58 to admixture THF with the MeCN-containing mobile phase reduced the extent of formation of the water enriched layer on the stationary phase.…”

Section: The Moderately Polar Aprotic Diluent For Analyte Injectionmentioning

confidence: 99%

Comparative study of different column types for the separation of polar basic hallucinogenic alkaloids

Ncube¹,

Poliwoda²,

Cukrowska³

et al. 2016

S.Afr.j.chem.

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The number of hallucinogenic compounds that have been separated simultaneously by liquid chromatography is limited. This research aimed to identify a column(s) that can allow for separation of several hallucinogens. The extent of separation of seven polar hallucinogenic tryptamine and phenethylamine derived alkaloids containing a basic N atom that becomes protonated at low pH values were investigated on five reverse-based columns and one hydrophilic interaction liquid chromatography (HILIC) column. An RP-phenyl and a negatively charged fused core HILIC were identified and recommended as effective columns in this regard. This research is the first to introduce a HILIC column in separation of hallucinogenic alkaloids and to simultaneously study the separation of the two principal psychoactive agents, muscimol and psilocin. The results of this study showed that better separation in HILIC columns can be achieved if an aprotic diluent of relatively lower polarity is used to introduce analytes into a mobile phase of higher polarity. Tetrahydrofuran was recommended where acetonitrile is used as the organic component of the mobile phase.

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Chromatographic behavior of epirubicin and its analogues on high-purity silica in hydrophilic interaction chromatography

Cited by 92 publications

References 26 publications

Characterization of enhanced-fluidity liquid hydrophilic interaction chromatography for the separation of nucleosides and nucleotides

Characterization of enhanced-fluidity liquid hydrophilic interaction chromatography for the separation of nucleosides and nucleotides

Effect of Temperature on the Chromatographic Behavior of Epirubicin and its Analogues on High Purity Silica Using Reversed-Phase Solvents

Comparative study of different column types for the separation of polar basic hallucinogenic alkaloids

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