Chromatographic Behavior of Selenoproteins in Rat Serum Detected by Inductively Coupled Plasma Mass Spectrometry

Anan, Yasumi; Hatakeyama, Yoshiko; Tokumoto, Maki; Ogra, Yasumitsu

doi:10.2116/analsci.29.787

Cited by 22 publications

(12 citation statements)

References 27 publications

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“…The peak detected at the retention time of 11.0 min was assigned to extracellular glutathione peroxidase (GPX3), and the peak at the retention time of 13.5 min was assigned to selenoprotein P (SELENOP) according to our previous literature [12]. The abbreviations of the selenoproteins (GPX3 and SELENOP) were adopted in accordance with the latest nomenclature [13].…”

Section: Resultsmentioning

confidence: 99%

“…Specifically, we evaluated the bioavailability from the recoveries of two serum selenoproteins, GPX3 and SELENOP, in Se-deficient rats by speciation analysis with an LC-ICP-MS. We have already established a speciation analysis for the determination of the two serum selenoproteins [12], which gives us more qualitative data than Western blotting and enzyme activity determination. In particular, the enzyme activity of SELENOP is still unknown.…”

Section: Discussionmentioning

confidence: 99%

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Bioavailability Comparison of Nine Bioselenocompounds In Vitro and In Vivo

Takahashi

Suzuki

Ogra

2017

IJMS

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Selenium (Se) shows biologically ambivalent characteristics in animals. It is an essential element but becomes severely toxic when the amount ingested exceeds the adequate intake level. Its biological, nutritional, and toxicological effects are strongly dependent on its chemical form. In this study, we evaluated the toxicity and bioavailability of nine naturally occurring Se compounds, or the so-called bioselenocompounds, in vivo and in vitro. Selenite and selenocystine showed higher toxicity than the other bioselenocompounds in vitro. In an in vitro membrane permeability study using Caco-2 cells, selenomethionine and Se-methylselenocysteine were more efficiently transported than the other bioselenocompounds. The effect of bioselenocompounds on nutritional availability was quantitatively determined from the recovery of serum selenoproteins in Se-deficient rats by speciation analysis. In contrast to the in vitro study, there were no significant differences in the assimilation of Se into serum selenoproteins among the bioselenocompounds, including selenoamino acids, selenosugar, and inorganic Se species, such as selenite, selenate, and selenocyanate, except trimethylselenonium ion. These results indicate that animals can equally assimilate both inorganic and organic naturally occurring selenocompounds except trimethylselenonium ion, which is the urinary metabolite of excess Se. We confirmed that the bioselenocompounds except trimethylselenonium ion had equivalent nutritional availabilities.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Bioavailability Comparison of Nine Bioselenocompounds In Vitro and In Vivo

Takahashi

Suzuki

Ogra

2017

IJMS

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“…The chromatographic behaviour of two mayor selenoproteins (extracellular gluthatione peroxidise and selenoprotein P) in serum of four rodent species HPLC (multi gel permeation column, 7.5 id Â 300 mm) coupled to ICP-MS and ICP-MS-MS have been compared. 183 Although the amino acid sequences in the rodent species is identical, different chromatographic behaviour was noted for selenoproteins derived from rat serum. Two well-separated Se-peaks were detected in rat serum whereas in mouse, hamster and guinea pig serum only one broad Sepeak was detected.…”

Section: Seleniummentioning

confidence: 99%

Atomic spectrometry updates. Review of advances in elemental speciation

Clough

Harrington

Hill

et al. 2014

J. Anal. At. Spectrom.

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“…35 This MO + mass shift improved detection limits for phosphorus (P), sulfur (S) and silicon (Si) over those obtained from isotope dilution (ID)-ICP-SF-MS in both aqueous 34 and organic matrices. 34,36 However if O 2 reaction has the potential to produce unwanted polyatomic species; then alternative reaction/collision gases may be used, e.g. NH 3 /He for the ultra-trace detection of titanium (Ti; as Ti(NH 3 ) 6 + ) in biological fluids.…”

Section: Introductionmentioning

confidence: 96%

Determination of selenium in serum in the presence of gadolinium with ICP-QQQ-MS

Bishop

Hare

Fryer

et al. 2015

Analyst

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Gadolinium (Gd)-based magnetic resonance imaging (MRI) contrasting agents interfere with the determination of selenium (Se) when analysed by single quadrupole inductively coupled plasma-mass spectrometry (ICP-MS). This paper demonstrates that an ICP-triple quadrupole-MS (ICP-QQQ-MS) with oxygen mass shift overcomes Gd(++) interference on Se(+) and mitigates typically encountered matrix and spectral based interferences. Normal human serum was diluted in a solution containing isopropanol, EDTA, NH4OH and Triton X-100. Samples were unspiked (control) serum; serum spiked with 0.127 μmol L(-1) Se or 127 μmol L(-1) Gd; and serum spiked with both 0.127 μmol L(-1) Se and 127 μmol L(-1) Gd. Consideration of collision/reaction gases and conditions for interference mitigation included helium (He); a 'low' and 'high' hydrogen (H2) flow, and oxygen (O2). The instrument tune for O2 was optimised for effective elimination of interferences via a mass shift reaction of Se(+) to SeO(+). The ICP-QQQ-MS was capable of detecting trace (>9.34 nmol L(-1)) levels of Se in serum in the presence of Gd in our simulated post-MRI serum sample. The multi-tune capabilities of the ICP-QQQ-MS may be adapted to eliminate other specific isobaric interferences that cause false positive results in other analyses where the analyte is confounded by doubly charged and/or polyatomic species.

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Chromatographic Behavior of Selenoproteins in Rat Serum Detected by Inductively Coupled Plasma Mass Spectrometry

Cited by 22 publications

References 27 publications

Bioavailability Comparison of Nine Bioselenocompounds In Vitro and In Vivo

Bioavailability Comparison of Nine Bioselenocompounds In Vitro and In Vivo

Atomic spectrometry updates. Review of advances in elemental speciation

Determination of selenium in serum in the presence of gadolinium with ICP-QQQ-MS

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