Chromatographic Determination of Azoxystrobin, Fenhexamid and Lufenur on Residues in Grapevine

Abstract

Residues of azoxystrobin (Amistar 25% SC), fenhexamid (Telidor 50% SC) and lufenuron (Match 5% EC) were determined on grapes treated with recommended doses. Grape leaves and fruit samples randomly collected after 1hr, 1, 3, 6, 10, 15 and 21 days of application, were extracted, cleaned-up and analyzed using chromatographic methods. Azoxystrobin, fenhexamid and lufenuron residues were dissipated on grape leaves after ten days of treatment by 87.83, 99.23 and 99.29% of the initial concentration, respectively. The corresponding values for dissipation of mentioned pesticides on grape fruits were 93.55, 99.62 and 99.99% of the initial concentration, respectively. The pre-harvest intervals (PHI) were calculated to be 6, 10 and 7 days after application of azoxystrobin, fenhexamid and lufenuron on grapes, respectively.

A novel validated simple derivatization liquid chromatographic method with diode array detection for the simultaneous determination of mancozeb, azoxystrobin and difenoconazole in pesticide dosage form

“…For the determination of mancozeb by iodometry, 12 square-wave adsorptive stripping voltammetry (SWAdSV), 13,14 batch injection analysis (BIA) with pulsed amperometric detection (PAD) on boron-doped diamond (BDD) electrode, 15 UV-spectrophotometry, 16–20 flow injection (FI) spectrophotometry, 21,22 photoacoustic and Fourier transform infrared spectroscopy, 23,24 gas chromatography (GLC) with specific detectors such as an electron capture detector (ECD), flame atomic absorption spectroscopic detector (FAAS) and flame photometric detector (FPD), 25,26 high performance liquid chromatography (HPLC), 27–35 gas chromatography-mass spectrometry (GC-MS), 36–42 and liquid chromatography-mass spectrometry (LC-MS) 43–49 methods have been reported. Similarly, various analytical methods for the quantification of azoxystrobin using UV-spectrophotometry, 50 flow injection chemiluminescence (FI-CL), 51 gas chromatography (GLC), 52–58 high-performance liquid chromatography (HPLC), 59–68 gas chromatography-mass spectrometry (GC-MS) 69–71 and liquid chromatography-mass spectrometry (LC-MS) 72–74 for single and multipesticide formulations were reported in literature. Furthermore, several methods for the quantification of Difenoconazole using voltammetric and flow amperometric detection on a boron-doped diamond electrode (FIA-ED), 75 UV-spectrophotometry, 76 gas chromatography (GLC), 77–83 high-performance liquid chromatography(HPLC), 84–87 gas chromatography-mass spectrometry (GC-MS) 88–92 and liquid chromatography-mass spectrometry (LC-MS) 93–99 methods were repored.…”