Chromatographic determination of monoterpenes and other acaricides in honeybees: Prevalence and possible synergies

Kasiotis, Konstantinos M.; Tzouganaki, Zampia; Machera, Kyriaki

doi:10.1016/j.scitotenv.2017.12.244

Cited by 32 publications

(23 citation statements)

References 46 publications

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“…Second large group used for this purpose is chromatographic methods, mostly coupled with MS [39,40,48,49]. A similar situation is in the case of thymol, where numerous procedures have been developed using electrochemical methods [41,46] and chromatographic methods coupled with MS [37,[43][44][45]. Determination of carvacrol is carried out mainly using chromatographic methods.…”

Section: Phenolic Terpenoids: Eugenol Carvacrol and Thymolmentioning

confidence: 99%

“…Due to the frequent combined occurrence of carvacrol and thymol in a number of matrices of natural origin-such as essential oils, honeys, complex medicinal products, or cosmetics-and the ever-increasing demand for such products, it is important to develop cheap, fast, and effective methods for simultaneous determination of phenolic terpenoids. Currently, only procedures based on an expensive chromatographic separation are used for this purpose [44,45]. Until now, voltammetric techniques have been used in work [41], where the authors employing square-wave voltammetry (SWV) and a glassy carbon electrode achieved the satisfactory results using artificial neural network (ANN) CH 3 HO C H 3 CH 3 CH 3 HO C H 3 CH 3 CH 3 HO eugenol carvacrol thymol to define multivariate calibration model for eugenol, carvacrol, and thymol.…”

Section: Phenolic Terpenoids: Eugenol Carvacrol and Thymolmentioning

confidence: 99%

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Multivariate approach in voltammetric identification and simultaneous determination of eugenol, carvacrol, and thymol on boron-doped diamond electrode

Kowalcze

Jakubowska

2019

Monatsh Chem

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The paper presents multivariate partial least-squares calibration model defined for the identification and simultaneous determination of phenolic terpenoids-eugenol, carvacrol, and thymol-by means of the differential pulse voltammetry. For the first time, using boron-doped diamond electrode signal, electrochemical distinction of carvacrol and thymol, despite the short distance between the peaks (15 mV), was described. The analytes' concentrations applied in the calibration model were selected using the 3-factorial central composite design. The multivariate partial least-squares model, with optimized data pre-processing, was used for determination of eugenol, carvacrol, and thymol in the concentration ranges of 0.77-13.86, 0.58-10.40, and 0.73-7.32 mg/dm 3 , respectively. The quality of the method, expressed by the root-mean-squared error of calibration was 0.55, 0.20, and 0.26 mg/dm 3 for the specified analytes. The recovery in the analysis of the pure signals was 98.92%, 101.62%, and 101.46%, what confirmed the possibility of clearly distinguishing the thymol and carvacrol peaks. In the spiked honey samples, the accuracy of eugenol, carvacrol, and thymol determination, expressed by the root-meansquared error of prediction, was in the range 0.20-0.83 mg/dm 3 , and recovery in the range 80.9-111.9%. Application of the presented model not only enables electrochemical identification and simultaneous determination of eugenol, carvacrol, and thymol, but also allows rapid, accurate, and low-cost verification of authenticity of the commercial products.

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Section: Phenolic Terpenoids: Eugenol Carvacrol and Thymolmentioning

confidence: 99%

Section: Phenolic Terpenoids: Eugenol Carvacrol and Thymolmentioning

confidence: 99%

Multivariate approach in voltammetric identification and simultaneous determination of eugenol, carvacrol, and thymol on boron-doped diamond electrode

Kowalcze

Jakubowska

2019

Monatsh Chem

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“…LC is generally applied to examine thermolabile, polar, and lowvolatility compounds, whereas GC can be used to determine thermostable and/or volatile compounds. Both of these chromatographic approaches are frequently coupled to mass spectrometry (MS), tandem mass spectrometry (MS/MS), or high-resolution mass spectrometry (HRMS) in order to achieve the maximum detection sensitivity (Kasiotis et al 2014(Kasiotis et al , 2018Bargańska and Namieśnik 2010;Gómez-Ramos et al 2019;Tong et al 2018;Niell et al 2017). However, other detection techniques can be employed for pesticide analysis.…”

Section: Pesticides and Organic Pollutantsmentioning

confidence: 99%

“…LC-based separation can be coupled to mass spectrometry detection by various interfaces, such as electrospray ionization (ESI) (Kasiotis et al 2018) or atmospheric pressure chemical ionization (APCI)-a soft ionization technique that yields quasimolecular ions and results in high detection sensitivity and information on molecular weight (Totti et al 2006). GC is often coupled to mass spectrometry by a "hard" electron ionization (EI) interface.…”

Section: Pesticides and Organic Pollutantsmentioning

confidence: 99%

“…The number of organic contaminants (not only pesticides) in the environment continues to rise each day, and new sample treatments (e.g., QuEChERS) are able to extract all of them, including unknown or untargeted pollutants such as metabolites of the targeted pollutants (Cotton et al 2014;Kasiotis et al 2018;Gómez-Ramos et al 2016. Mass spectrometers operating in multiple reaction monitoring (MRM) mode cannot detect all of these compounds, so researchers prefer to use HRMS detection, which is performed in a full-scan mode such as time-of-flight (TOF), Fourier transform ion cyclotron resonance (FT-ICR), or Orbitrap analysis.…”

Section: Pesticides and Organic Pollutantsmentioning

confidence: 99%

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Bioindicators and biomonitoring: honeybees and hive products as pollution impact assessment tools for the Mediterranean area

Girotti

Ghini

Ferri

et al. 2020

Euro-Mediterr J Environ Integr

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The global range of the environmental quality issues we all face necessitates integrated action from all of the countries that suffer from a particular environmental issue. We must be able to obtain data on the presence of pollutants and their effects on biota from any territory, regardless of its environmental, infrastructural, social, and economic conditions. Biomonitoring utilizes organisms and natural materials to obtain this information. In particular, the honeybee is a ubiquitous, easy-to-breed organism with great mobility. Its body, which is covered with hairs, picks up materials and particulates that it encounters in the environment. Therefore, bees are highly effective accumulators of materials from the soil, vegetation, air, and water. These characteristics mean that the honeybee is both a bioindicator and a passive bioaccumulator organism, making it an ideal agent for easily monitoring vast areas inexpensively, even in regions where infrastructure is scarce. In this short review, we summarize the main targets of the honeybee-based monitoring campaigns that have been carried out to date, highlighting the results obtained in assessments of organic and inorganic pollution performed by coupling more modern technologies with this long-standing practice. It is hoped that this review will make scientists more aware of the incredible potential of these delicate organisms to provide data that could prove useful in the management of environmental issues.

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The use of the Hazard Quotient approach to assess the potential risk to honeybees (Apis mellifera) posed by pesticide residues detected in bee‐relevant matrices is not appropriate

Thompson

2021

Pest Management Science

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BACKGROUND Pesticide residue data for pollen and nectar are valuable for characterizing realistic exposure of pollinators, e.g. from agricultural crops, flowering margins. Interpretation of residues relies on comparing exposure with toxicity and the Hazard Quotient (HQ) is widely utilized. However, the HQ (threshold of concern 50) was only validated for foliar sprays, based on application rate as a proxy for exposure, not measured residues in bee‐relevant matrices. RESULTS A review of the literature showed a range of HQ approaches and thresholds of concern used to assess non‐foliar applications and residues detected in bee‐relevant matrices, mostly pollen. The use of the HQ thresholds to assess risks associated with residue data or non‐foliar spray application methods is not validated, does not reflect realistic exposure and the conclusions reached differ substantially from current risk assessment approaches. Re‐evaluation of residue data from the first published use of the concentration‐based HQ (2013) and a recent paper (2021) reduced the proportion of pesticides where a conclusion of potential risk was reached from 30 to 7% and 28% to 3–6%, respectively. CONCLUSIONS An understanding of the applicability of the selected risk assessment approach to the available residue data is needed to enable robust conclusions to be drawn on the potential risk to bees. Use of the HQ approach to assess the risk posed by application methods other than foliar sprays or residues in nectar and pollen is likely to result in unreliable conclusions. An alternative approach should be used to assess the significance of measured residues.

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Chromatographic determination of monoterpenes and other acaricides in honeybees: Prevalence and possible synergies

Cited by 32 publications

References 46 publications

Multivariate approach in voltammetric identification and simultaneous determination of eugenol, carvacrol, and thymol on boron-doped diamond electrode

Multivariate approach in voltammetric identification and simultaneous determination of eugenol, carvacrol, and thymol on boron-doped diamond electrode

Bioindicators and biomonitoring: honeybees and hive products as pollution impact assessment tools for the Mediterranean area

The use of the Hazard Quotient approach to assess the potential risk to honeybees (Apis mellifera) posed by pesticide residues detected in bee‐relevant matrices is not appropriate

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