Chromatographic determination of some tropane alkaloids in Datura metel

Темердашев, А. З.; Kolychev, I. A.; Киселева, Н. В.

doi:10.1134/s1061934812120040

Cited by 24 publications

(16 citation statements)

References 12 publications

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“…Among the techniques used, High Performance Liquid Chromatography (HPLC) is the most widely developed for this purpose (17)(18)(19)(20)(21)(22)(23)(24). Buffered mobile systems or having only one salt dissolved in their composition (K2HPO4, KH2PO4, for example) are best described for quantifying tropic type alkaloids (18)(19)(20)(21)(22)(23). Ashtiania and Sefidkonb (2011) (21) quantified total alkaloids (atropine and scopolamine) in A. belladonna leaf cultures using these systems.…”

Section: Introductionmentioning

confidence: 99%

Quantification of Atropine in Leaves of Atropa Belladonna: Development and Validation of Method by High-Perfomance Liquid Chromatography (Hplc)

et al. 2017

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The leaves of Atropa belladonna (L) are characterized by the presence of the alkaloid atropine, known for the antimuscarinic activity. The leaves are used in therapy, mainly in homeopathic preparations. An HPLC method was developed and validated to quantify atropine in belladonna leaves. The samples were extracted with methanol, followed by acid-base extraction with 5% HCl and dichloromethane. Analysis by HPLC was performed on C18 column, in a linear gradient system using a system with two mobile phases (water and acetonitrile), both acidified with trifluoroacetic acid. The determinations were performed using a reference standard and a diode array detector at 210 nm. The method was validated and proved to be specific / selective, comparing the UV profiles and the purity of the atropine peaks in reference and sample solutions and analyzing sample solutions with and without addition of standard, which produced an increase only of the area of peak of the sample, without changing the area of the adjacent peaks. The linearity (50 - 200 μg/mL) was provided by analysis of the analytical curves of atropine, with r2 = 0.9996. LOD and LOQ were 3.75 and 11.4 µg/ml, respectively. The method was precise, reproducible and accurate, and presented recovery equal to 103.0%. The method was considered robust for the analyzed parameters. Four commercial samples were analyzed and the mean levels of atropine found ranged from 0.16 - 0.27 %. Thus, the developed method is effective for quantification of atropine in beladona leaves and meets the validation requirements of current legislation and ensuring the quality control of the plant material.

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Section: Introductionmentioning

confidence: 99%

Quantification of Atropine in Leaves of Atropa Belladonna: Development and Validation of Method by High-Perfomance Liquid Chromatography (Hplc)

et al. 2017

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“…In the case of LC, MS is the predominant detection system used due to its high selectivity and sensitivity. Several methods for the analysis of TAs in Solanaceae plants [17][18][19], body fluids [20][21][22][23], cereals crops [8,19,24], and herbal tea and tea [12,19,20] are available, allowing the determination of these compounds at μg/kg or μg/L levels using LC-MS. None of the above methods analyzed more than seven TAs, except the study performed by Mulder [19], and most of them only quantified scopolamine and atropine.…”

mentioning

confidence: 99%

“…Most of the reported extraction methods are only available for scopolamine and atropine [12,17,24] and there are some multianalyte methods [7,8,19,20,31] using mixtures of methanol/water or acetonitrile/water. In some studies, TAs are extracted using SPE, with different sorbents as Diapac C 18 [18], Cartridges SolEx HRP [31], or Oasis MCX [19,32].…”

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confidence: 99%

Simultaneous analysis of tropane alkaloids in teas and herbal teas by liquid chromatography coupled to high‐resolution mass spectrometry (Orbitrap)

Romera-Torres

Romero‐González

Vidal

et al. 2018

J of Separation Science

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A new method has been developed for the simultaneous determination of 13 tropane alkaloids in tea and herbal teas using high-performance liquid chromatography coupled to an Exactive-Orbitrap analyzer. A mixture of methanol, water, and formic acid was used for the extraction of the target compounds followed by a solid-phase extraction step. The validated method provided recoveries from 75 to 128% with intra- and interday precision lower than or equal to 24% (except for apoatropine). Limits of quantification ranged from 5 to 20 μg/kg. Eleven tea and herbal tea samples and two contaminated samples with Datura stramonium seeds were analyzed. Tropane alkaloids were detected in six samples with concentrations from 5 (apoatropine) to 4340 μg/kg (sum of physoperuvine, pseudotropine, and tropine), whereas concentrations from 5 (apoatropine) to 1725 μg/kg (sum of physoperuvine, pseudotropine, and tropine) were found in the contaminated samples.

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“…Almost no peak broadening was observed, superior to merely using TFA as additive. Determination of tropane alkaloids in different tissues of Datura metel L. was implemented in eluent system consisting of ACN, phosphate buffer, and isopropanol [22].…”

Section: Chromatographic Separation Methodsmentioning

confidence: 99%

Liquid chromatographic separation of alkaloids in herbal medicines: Current status and perspectives

Zhao

Chen

Yu-qian

et al. 2020

J of Separation Science

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Alkaloids are a widespread group of basic compounds in herbal medicines and have attracted great interest due to various pharmaceutical activities and desirable druggability. Their distinctive structures make chromatographic separation fairly difficult. Peak tailing, poor resolution, and inferior column‐to‐column reproducibility are common obstacles to overcome. In order to provide a valuable reference, the methodologies and/or strategies on liquid chromatographic separation of alkaloids in herbal medicines proposed from 2012 to 2019 are thoroughly summarized.

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Chromatographic determination of some tropane alkaloids in Datura metel

Cited by 24 publications

References 12 publications

Quantification of Atropine in Leaves of Atropa Belladonna: Development and Validation of Method by High-Perfomance Liquid Chromatography (Hplc)

Quantification of Atropine in Leaves of Atropa Belladonna: Development and Validation of Method by High-Perfomance Liquid Chromatography (Hplc)

Simultaneous analysis of tropane alkaloids in teas and herbal teas by liquid chromatography coupled to high‐resolution mass spectrometry (Orbitrap)

Liquid chromatographic separation of alkaloids in herbal medicines: Current status and perspectives

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