Chromatographic Determination of Stevioside in Raw Plant Material

Кедик, С. А.; Федоров, С. В.; Yanul, N. A.; Prokhorova, L. V.; Смирнова, Е. В.; Панов, Алексей Валерьевич

doi:10.1023/b:phac.0000014857.33465.d7

Cited by 8 publications

(5 citation statements)

References 7 publications

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“…S2 shows the improvised process of isolation and purification process of steviosides with recovery yield of sweet glycosides in the range of 85–98%, of which steviosides‐A (85–95%) and rebaudioside‐A (3–8%) and the purity and the chemical structure of the stevioside confirmed through HPLC, LC‐Mass and NMR spectral analysis and compared with literature data. (Kedik et al ., ; Chaturvadula et al ., ; Puri et al ., ) The melting point of the isolated white stevioside powder was 197–199 °C and observed spectral data of the product was 1 H NMR (300 MHz) δ0.98 (s, 3H, C 20 CH 3 ), 1.21 (s, 3HC 18 ‐CH 3 ), 4.86 (S, 1H, C 17 ‐H) 5.21 (S, 1H, C 17 ‐H) 4.59 (D, J = 7.9 Hz, 1H) 5.38 (D, J = 7.9 Hz, 1H); +ESIMS (M + Na) + m/z 827.3891 (calcd. for C38H60O18Na:827.3891).…”

Section: Resultsmentioning

confidence: 99%

An improvised process of isolation, purification of steviosides from Stevia rebaudiana Bertoni leaves and its biological activity

Rao

Reddy

Prasad

et al. 2012

Int J of Food Sci Tech

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The food industry has traditionally used sugar (sucrose) as a sweetening agent; however the dietary and health demands are continuing to expand the market for sweeteners as an alternative to sucrose. The leaves of Stevia rebaudiana are rich source of glycosides that have sweet taste with low calorific value. The study highlights extraction of steviosides from leaves using pressurised hot water extractor, followed by purification and concentration of the sweet glycosides through ultra (UF) and nano (NF) filtration membrane in obtaining high purity steviosides. After the final purification process the stevioside content was 9.05 g per 100 g and rebaudioside A 0.2 g per 100 g stevia leaf, with total purity of stevioside 97.66% by HPLC at total operation time of 7 h. This process also improved the potency of sweetness and palatability profile when compare with other commercially available steviosides. Thus, the methodology developed establishes simple, in-expensive and eco-friendly process in obtaining pure steviosides.

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Section: Resultsmentioning

confidence: 99%

An improvised process of isolation, purification of steviosides from Stevia rebaudiana Bertoni leaves and its biological activity

Rao

Reddy

Prasad

et al. 2012

Int J of Food Sci Tech

View full text Add to dashboard Cite

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“…The eluent flow rate velocity was 0.9 mL min −1 . The wavelength of UV detector was 203 nm [26]. The standard reference material for ST was purchased from the National Institute for Food and Drug Control (HPLC≥99%).…”

Section: Methodsmentioning

confidence: 99%

Purple Phototrophic Bacterium Enhances Stevioside Yield by Stevia rebaudiana Bertoni via Foliar Spray and Rhizosphere Irrigation

Wang

Lin

2013

PLoS ONE

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This study was conducted to compare the effects of foliar spray and rhizosphere irrigation with purple phototrophic bacteria (PPB) on growth and stevioside (ST) yield of Stevia. rebaudiana. The S. rebaudiana plants were treated by foliar spray, rhizosphere irrigation, and spray plus irrigation with PPB for 10 days, respectively. All treatments enhanced growth of S. rebaudiana, and the foliar method was more efficient than irrigation. Spraying combined with irrigation increased the ST yield plant -1 by 69.2% as compared to the control. The soil dehydrogenase activity, S. rebaudiana shoot biomass, chlorophyll content in new leaves, and soluble sugar in old leaves were affected significantly by S+I treatment, too. The PPB probably works in the rhizosphere by activating the metabolic activity of soil bacteria, and on leaves by excreting phytohormones or enhancing the activity of phyllosphere microorganisms.

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“…The sample aliquots were subjected to analysis as a dry extract evaluation and analysis using high-performance liquid chromatography with a diode-array detector (HPLC/DAD) [32,33]. For the analysis of the dry extract, 10 mL of sample was placed in an oven until a constant weight was obtained.…”

Section: Hplc Analysismentioning

confidence: 99%

Comparison between Two Solid-Liquid Extraction Methods for the Recovery of Steviol Glycosides from Dried Stevia Leaves Applying a Numerical Approach

Gallo

Formato

2018

Processes

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Stevia rebaudiana Bertoni is a perennial shrub belonging to the Asteraceae family. The leaves contain a mixture of steviol glycosides with extraordinary sweetening properties, among which the most important are stevioside and rebaudioside A. These components have a high sweetening power, which is about 300 times that of sucrose, and a negligible calorie content. However, their extraction and purification are not easy. In this paper, the extraction technique under cyclic pressure, known as rapid solid-liquid dynamic extraction (RSLDE), was compared using a Naviglio extractor (NE) with conventional maceration. The aim was to identify an efficient and economically viable method for obtaining high amounts of steviol glycosides in a short time. Furthermore, a numerical model was set up for the solid-liquid extraction process of value-added compounds from natural sources. Several parameters must be evaluated in relation to the characteristics of the parts of the plant subjected to extraction. Therefore, since diffusion and osmosis are highly dependent on temperature, it is necessary to control the temperature of the extraction system. On the other hand, the final aim of this work was to provide a scientific and quantitative basis for RSLDE. Therefore, the results obtained from stevia extracts using the corresponding mathematical model allowed hypothesizing the application of this model to the extraction processes of other vegetable matrices.

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Chromatographic Determination of Stevioside in Raw Plant Material

Cited by 8 publications

References 7 publications

An improvised process of isolation, purification of steviosides from Stevia rebaudiana Bertoni leaves and its biological activity

An improvised process of isolation, purification of steviosides from Stevia rebaudiana Bertoni leaves and its biological activity

Purple Phototrophic Bacterium Enhances Stevioside Yield by Stevia rebaudiana Bertoni via Foliar Spray and Rhizosphere Irrigation

Comparison between Two Solid-Liquid Extraction Methods for the Recovery of Steviol Glycosides from Dried Stevia Leaves Applying a Numerical Approach

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