Chromatographic fractionation of Aspergillus endotoxins

Wynston, Leslie K.; Tilden, Evelyn B.

doi:10.1007/bf02089214

Cited by 13 publications

(4 citation statements)

References 14 publications

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“…The molecular weight of Asp-hemolysin thus obtained was estimated to be 30,000 by the gel filtration technique. This molecular weight is similar to that of the results of Wynston et al (17) who reported values of 29,400 for the molecular weight of the hemolytic fractionfrom A. fumigatus as determined by the ultracentrifugation technique. The optimum pH of the Asp-hemolysin was in the acid region, pH 5.0, and its optimum temperature was around room temperature (21 C).…”

Section: Discussionsupporting

confidence: 91%

“…The mycelial extracts were heat-labile toxins possessing antigenicity and lethality against the animals tested. This study has been extended by 16,17), and they have suggested the presence of two different toxins, each having hemolytic or nephrotoxic activity, in the mycelia of A. fumigatus and A. flavus. Iwata et al (5) isolated an endotoxin-like substance which showed lethal toxicity against mice from mycelia of A. fumigatus grown 24 hr on Czapek's medium.…”

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confidence: 74%

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Studies on the Toxin of Aspergillus fumigatus

Yokota

Shimada

Kamaguchi

et al. 1977

Microbiology and Immunology

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A hemolytic toxin has been obtained from mycelia and culture filtrates of Asper gillus fumigatus by the procedures that included precipitation with ammonium sulfate, chromatography of DEAE-Sephadex, affinity chromatography on Concanavalin A-Sepharose and gel filtration on Sephadex G-50, G-100 and G-150. The purified homolytic toxin was homogeneous on immunological and disk electrophoretic analysis, and the toxin from culture filtrates was identical with that from mycelia by the immunodiffusion technique. The hemolytic toxin was obtained for the first time from fungi and designated as Asp-hemolysin. The molecular weight of Asp-hemolysin was estimated to be approximately 30,000 by the gel-filtration technique and its isoelectric point was found to be around pH 4.0. This Asphemolysin contained large amounts of protein and very small amounts of carbohydrate. The UV absorption spectrum of Asp-hemolysin showed a maximum absorption at 280 nm and minimum absorption at 251 nm. The extinction coefficient at 280 nm, E was 12.4 and the ratio of absorbance at 280 nm to that at 260 nm was 2.3. The optimum pH for the hemolytic activity of the toxin toward chicken erythrocytes was 5.0 at room temperature and it was active in the pH range of 3.5 to 10.5. The optimum temperature was 21 C and about 50% of the activity was lost by incubation at 50 C for 5 min or 45 C for 23 min. The hemolytic activity was remarkably inhibited by Hg2+, Cu2+, Fe2+, Ag1+, iodine and p-CMB, but enhanced slightly by Zn2+ and Co2+.In 1939, Henrici (4) first reported the biological properties of crude endotoxins extracted from the mycelia of Aspergillus fumigatus. The mycelial extracts were heat-labile toxins possessing antigenicity and lethality against the animals tested. This study has been extended by 16,17), and they have suggested the presence of two different toxins, each having hemolytic or nephrotoxic activity, in the mycelia of A. fumigatus and A. flavus. Iwata et al (5) isolated an endotoxin-like substance which showed lethal toxicity against mice from mycelia of A. fumigatus grown 24 hr on Czapek's medium.

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Section: Discussionsupporting

confidence: 91%

mentioning

confidence: 74%

Studies on the Toxin of Aspergillus fumigatus

Yokota

Shimada

Kamaguchi

et al. 1977

Microbiology and Immunology

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“…From the methods used it can be understood that the fungal extracts employed in this investigation were crude, and largely expressed sap from mycelial mats. No purification of toxic materials was made on the scale carried out by Tilden et al (1961), Tilden, Freeman, and Lombard (1963), Wynston and Tilden (1963), and Rau, Tilden, and Koenig (1961). Therefore little direct comparison can be made between present results and those recorded by the Chicago workers.…”

Section: Haematology and Biochemistrymentioning

confidence: 59%

A nephrotoxin fromAspergillus fumigatusand its possible relationship with New Zealand mucosal disease-like syndrome in cattle

Thornton¹,

Shirley²,

Salisbury³

1968

New Zealand Journal of Agricultural Research

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Extracts of mycelium of Aspergillus fumigatus, when injected intra-vC'1ously into mice and sheep, caused renal cortical necrosis, with or without peri-renal oedema, and lesions of the alimentary tract in sheep.These lesions were very similar to those described for the New Zealand mucosal disease-like syndrome affecting cattle.When extracts and cultures of the fungus were dosed per os to ~heep and a calf there was no evidence of kidney damage as shown by blood analysis.

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“…This finding is in agreement with that of Tilden et al (1961Tilden et al ( , 1963, and would indicate that the haemolysin and the toxin are different principles. Although Wynston & Tilden (1963), using chromatography, could not completely separate haemolysin and toxin, they found the greatest haemolytic and toxic activity in different fractions. The dermo-necrotic effect of the AF filtrate in the rabbit proved to be bound to the haemolysin (Tilden et al 1963).…”

Section: Discussionmentioning

confidence: 90%

Studies on Aspergillus Fumigatus; Stability of Haemolysin and Toxin in Crude Filtrate

Rutqvist

1968

Acta Vet Scand

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Chromatographic fractionation of Aspergillus endotoxins

Cited by 13 publications

References 14 publications

Studies on the Toxin of Aspergillus fumigatus

Studies on the Toxin of Aspergillus fumigatus

A nephrotoxin fromAspergillus fumigatusand its possible relationship with New Zealand mucosal disease-like syndrome in cattle

Studies on Aspergillus Fumigatus; Stability of Haemolysin and Toxin in Crude Filtrate

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