Chromatographic Microassay for Cholesterol and Cholesterol Esters

Wycoff, H. D.; Parsons, J. H.

doi:10.1126/science.125.3243.347

Cited by 71 publications

(16 citation statements)

References 3 publications

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“…This chloroform was evaporated under a stream of nitrogen and the dry material was dissolved in glacial acetic acid. The amount of cholesterol in solution was determined by the FeCl, reaction (Wycoff & Parsons, 1957).…”

Section: Organismsmentioning

confidence: 99%

Chemical Composition of Mycoplasma Cells and Membranes

Razin¹,

Argaman²,

Avigan³

1963

Journal of General Microbiology

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Section: Organismsmentioning

confidence: 99%

Chemical Composition of Mycoplasma Cells and Membranes

Razin¹,

Argaman²,

Avigan³

1963

Journal of General Microbiology

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“…Free cholesterol was separated from its esters by thin-layer chromatography ; Argaman & Razin, to be published) and estimated by the FeC1, reaction (Wycoff & Parsons, 1957).…”

mentioning

confidence: 99%

Lipase Activity of Mycoplasma

Rottem

Razin

1964

Journal of General Microbiology

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SUMMARYAll of eight Mycoplasma strains tested were capable of hydrolysing tributyrin. The saprophytic Mycoplasma laidlawii strains showed the lowest lipolytic activity, and the parasitic Mycoplasrna gallisepticum the highest. The properties of M . gallisepticum lipase were studied in some detail. The lipolytic activity of this organism was highest at the logarithmic phase of growth and declined steeply afterwards. The enzyme was not bound to the cell membrane and appeared in the soluble fraction of disrupted cells. The cell extract hydrolysed tributyrin much faster than trilaurin or triolein. Methyl oleate and 'Tween 80' were only slowly hydrolysed. Cholesteryl acetate and stearate were not hydrolysed by cell extract or by intact M . gallisepticum. Partial purification of the lipase was accomplished by ammonium sulphate fractionation of cell-extract proteins, followed by anion-exchange chromatography. The partially purified enzyme did not require inorganic ions for activity and its optimal pH value varied between 7-5 and 8-0, depending on the substrate tested.

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“…The first-named fraction showed a long-spacing of 41.7A (~+ 0.2), which corresponds with the spacing to be expected for a mixture of Ca1 and C33 paraffins in the proportion 90:10 (13). The acid fraction showed a long spacing of 81.0 A (--+ 0.2), corresponding with that of an equimolecular mixture of C3o, Cs2, and C34 acids (14). The presence of free alcohols could not be demonstrated.…”

Section: Chromatography Columns With a Diameter Of 24mentioning

confidence: 84%