Chromatographic profiles of extractives from leaves of Eugenia uniflora

Bezerra, Isabelle Cristinne Ferraz; Ramos, Rhayanne Thaís de Moraes; Ferreira, Magda Rhayanny Assunção; Soares, Luiz Alberto Lira

doi:10.1016/j.bjp.2017.11.002

Cited by 38 publications

(39 citation statements)

References 35 publications

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“…The compounds most representing the metabolomics-based dataset (in terms of relative abundance) were two monoterpenoids (i.e., bornyl diphosphate and isobornyl isobutyrate), three polyphenols (i.e., cinnamic acid, umbelliferone, and caffeic acid), the alkaloid lansiumamide C, and the lysophospholipids LysoPE (0:0/18:2(9Z,12Z)). Overall, the compounds putatively annotated in our experimental conditions were in agreement with the chemical composition recently reported in literature for pitanga leaves ( E. uniflora ), when considering the most important classes [ 27 , 30 , 31 ]. In fact, PLE is reported to be a source of flavonoids followed by tannins and terpenoids.…”

Section: Resultssupporting

confidence: 90%

“…In particular, a great abundance of diglycerides and phospholipids (i.e., glycero- and lysophospholipids), linoleic acid derivatives (such as 18-oxo-oleate), and prenol lipids was observed. Therefore, the results gained from our PLE extracts using untargeted metabolomics provided a wider compounds coverage than targeted approaches [ 27 , 30 ], thus highlighting the rich distribution of antioxidant compounds in this plant matrix. In a previous work [ 29 ], a PLE was tested as natural antioxidant for enhancing canola oil stability by monitoring lipid oxidation parameters.…”

Section: Resultsmentioning

confidence: 99%

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Impact of a Pitanga Leaf Extract to Prevent Lipid Oxidation Processes during Shelf Life of Packaged Pork Burgers: An Untargeted Metabolomic Approach

Rocchetti

Bernardo

Pateiro

et al. 2020

Foods

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In this work, the comprehensive metabolomic changes in pork burgers treated with different antioxidants, namely, (a) a control without antioxidants, (b) 200 mg/kg butylated hydroxytoluene (BHT), and (c) 250 mg/kg pitanga leaf extract (PLE, from Eugenia uniflora L.), each one packaged under modified atmosphere (80% O2 and 20% CO2) for 18 days storage at 2 ± 1 °C, were deeply studied. In particular, untargeted metabolomics was used to evaluate the impact of the antioxidant extracts on meat quality. The PLE phytochemical profile revealed a wide variety of antioxidant compounds, such as polyphenols, alkaloids, and terpenoids. Multivariate statistics (both unsupervised and supervised) allowed to observe marked differences in BHT and PLE burgers metabolomic profiles during storage. Most of the differences could be attributed to hexanoylcarnitine, 4-hydroxy-2-nonenal, 6-hydroxypentadecanedioic acid, 9S,11S,15S,20-tetrahydroxy-5Z,13E-prostadienoic acid (20-hydroxy-PGF2a), sativic acid, followed by glycerophospholipids. In addition, significant correlations (p < 0.01) were observed between thiobarbituric acid reactive substances and metabolites related to lipid oxidation processes. Therefore, the approach used showed a clear modulation of lipid oxidation, likely promoted by the plant leaf extract, thus confirming the ability of PLE to delay lipid oxidative phenomena during storage.

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Section: Resultssupporting

confidence: 90%

Section: Resultsmentioning

confidence: 99%

Impact of a Pitanga Leaf Extract to Prevent Lipid Oxidation Processes during Shelf Life of Packaged Pork Burgers: An Untargeted Metabolomic Approach

Rocchetti

Bernardo

Pateiro

et al. 2020

Foods

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“…After development, the plates were dried and the compounds were visualized after derivatization by spraying the NEU+PEG reagent for flavonoids and hydolysable tannins, and vanillin chloride for condensed tannins. Were used as standards: quercetin, gallic acid and catechin, respectively, for flavonoids, hydrolysable tannins and condensed tannins (Bezerra et al, 2018).…”

Section: Phytochemical Analysis: Thin Layer Chromatography (Tlc)mentioning

confidence: 99%

In vitro antifungal activity and phytochemical characterization of Eugenia uniflora, Libidibia ferrea and Psidium guajava

Ferreira

Santiago

Silva-Rocha

et al. 2020

Braz. J. Pharm. Sci.

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“…Compared to manual TLC, the technique yields better reproducibility, resolution and sensitivity, particularly when combined with improved digital scanning and documentation software, resulting in a more complete extraction of the information. In combination with chemometric data analysis, HPTLC has been reported to be a powerful tool for statistical analysis of the chemical profiles of herbal medicines, and for exploring differences and similarities within individual samples [ 9 , 10 , 11 ]. The development of rTLC software, available as an open-source web package for image processing, presents new opportunities for chemometric analysis of HPTLC chromatograms.…”

Section: Introductionmentioning

confidence: 99%

Phytochemical Profiling and Quality Control of Terminalia sericea Burch. ex DC. Using HPTLC Metabolomics

et al. 2021

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Terminalia sericea is used throughout Africa for the treatment of a variety of conditions and has been identified as a potential commercial plant. The study was aimed at establishing a high-performance thin layer chromatography (HPTLC) chemical fingerprint for T. sericea root bark as a reference for quality control and exploring chemical variation within the species using HPTLC metabo3lomics. Forty-two root bark samples were collected from ten populations in South Africa and extracted with dichloromethane: methanol (1:1). An HPTLC method was optimized to resolve the major compounds from other sample components. Dichloromethane: ethyl acetate: methanol: formic acid (90:10:30:1) was used as the developing solvent and the plates were visualized using 10% sulfuric acid in methanol as derivatizing agent. The concentrations of three major bioactive compounds, sericic acid, sericoside and resveratrol-3-O-β-rutinoside, in the extracts were determined using a validated ultra-performance liquid chromatography-photodiode array (UPLC-PDA) detection method. The rTLC software (written in the R-programming language) was used to select the most informative retardation factor (Rf) ranges from the images of the analysed sample extracts. Further chemometric models, including principal component analysis (PCA) and hierarchical cluster analysis (HCA), were constructed using the web-based high throughput metabolomic software. The rTLC chemometric models were compared with the models previously obtained from ultra-performance liquid chromatography coupled with mass spectrometry (UPLC-MS). A characteristic fingerprint containing clear bands for the three bioactive compounds was established. All three bioactive compounds were present in all the samples, although their corresponding band intensities varied. The intensities correlated with the UPLC-PDA results, in that samples containing a high concentration of a particular compound, displayed a more intense band. Chemometric analysis using HCA revealed two chemotypes, and the subsequent construction of a loadings plot indicated that sericic acid and sericoside were responsible for the chemotypic variation; with sericoside concentrated in Chemotype 1, while sericic acid was more abundant in Chemotype 2. A characteristic chemical fingerprint with clearly distinguishable features was established for T. sericea root bark that can be used for species authentication, and to select samples with high concentrations of a particular marker compound(s). Different chemotypes, potentially differing in their therapeutic potency towards a particular target, could be distinguished. The models revealed the three analytes as biomarkers, corresponding to results reported for UPLC-MS profiling and thereby indicating that HPTLC is a suitable technique for the quality control of T. sericea root bark.

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Chromatographic profiles of extractives from leaves of Eugenia uniflora

Cited by 38 publications

References 35 publications

Impact of a Pitanga Leaf Extract to Prevent Lipid Oxidation Processes during Shelf Life of Packaged Pork Burgers: An Untargeted Metabolomic Approach

Impact of a Pitanga Leaf Extract to Prevent Lipid Oxidation Processes during Shelf Life of Packaged Pork Burgers: An Untargeted Metabolomic Approach

In vitro antifungal activity and phytochemical characterization of Eugenia uniflora, Libidibia ferrea and Psidium guajava

Phytochemical Profiling and Quality Control of Terminalia sericea Burch. ex DC. Using HPTLC Metabolomics

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