Chromatographic profiling of Pancharishta at different stages of its development using HPTLC, HPLC, GC–MS and UPLC–MS

Khan, Washim; Chester, Karishma; Anjum, Varisha; Ahmad, Wasim; Ahmad, Sayeed; Narwaria, Avinash; Kumar, Das Prasanta; Katiyar, Chandrakant

doi:10.1016/j.phytol.2017.04.034

Cited by 22 publications

(10 citation statements)

References 24 publications

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“…Extract at a dose of 100 mg/kg was orally administered and blood samples were collected at different time intervals (0, 1, 2, 4, 8, and 12 h) from a retro-orbital vein. Collected blood samples were subjected to centrifuge at 4,000 rpm for 10 min and serum was separated ( Khan et al, 2016a , b ).…”

Section: Methodsmentioning

confidence: 99%

Hypoglycemic Potential of Aqueous Extract of Moringa oleifera Leaf and In Vivo GC-MS Metabolomics

et al. 2017

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Moringa oleifera Lam. (family; Moringaceae), commonly known as drumstick, have been used for centuries as a part of the Ayurvedic system for several diseases without having any scientific data. Demineralized water was used to prepare aqueous extract by maceration for 24 h and complete metabolic profiling was performed using GC-MS and HPLC. Hypoglycemic properties of extract have been tested on carbohydrate digesting enzyme activity, yeast cell uptake, muscle glucose uptake, and intestinal glucose absorption. Type 2 diabetes was induced by feeding high-fat diet (HFD) for 8 weeks and a single injection of streptozotocin (STZ, 45 mg/kg body weight, intraperitoneally) was used for the induction of type 1 diabetes. Aqueous extract of M. oleifera leaf was given orally at a dose of 100 mg/kg to STZ-induced rats and 200 mg/kg in HFD mice for 3 weeks after diabetes induction. Aqueous extract remarkably inhibited the activity of α-amylase and α-glucosidase and it displayed improved antioxidant capacity, glucose tolerance and rate of glucose uptake in yeast cell. In STZ-induced diabetic rats, it produces a maximum fall up to 47.86% in acute effect whereas, in chronic effect, it was 44.5% as compared to control. The fasting blood glucose, lipid profile, liver marker enzyme level were significantly (p < 0.05) restored in both HFD and STZ experimental model. Multivariate principal component analysis on polar and lipophilic metabolites revealed clear distinctions in the metabolite pattern in extract and in blood after its oral administration. Thus, the aqueous extract can be used as phytopharmaceuticals for the management of diabetes by using as adjuvants or alone.

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Section: Methodsmentioning

confidence: 99%

Hypoglycemic Potential of Aqueous Extract of Moringa oleifera Leaf and In Vivo GC-MS Metabolomics

et al. 2017

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“…To derivatize a range of functional metabolites, two stages are involved in [79]. First, an oxime is formed, followed by trimethylsilylation [33,80]. The derivatization process can be hindered both by extensive sample drying [33] and the presence of water [76].…”

Section: Gc-msmentioning

confidence: 99%

Analytical Techniques for Metabolomic Studies: A Review

et al. 2019

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Metabolomics is the comprehensive study of small-molecule metabolites. Obtaining a wide coverage of the metabolome is challenging because of the broad range of physicochemical properties of the small molecules. To study the compounds of interest spectroscopic (NMR), spectrometric (MS) and separation techniques (LC, GC, supercritical fluid chromatography, CE) are used. The choice for a given technique is influenced by the sample matrix, the concentration and properties of the metabolites, and the amount of sample. This review discusses the most commonly used analytical techniques for metabolomic studies, including their advantages, drawbacks and some applications.

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“…With the advancement of MS-imaging techniques, including matrix-assisted laser desorption (MALDI) and desorption electrospray (DESI) ionization platforms combined with high-resolution MS, it is feasible to conduct in-situ metabolome analysis (Blanksby and Mitchell 2010). Therefore, for liquid phase separations, ultrahigh performance liquid chromatography (UPLC) and high-performance-LC (HPLC) are used for metabolite analysis in various applications; these are a robust analytical method that permits the discovery of plant metabolites when it is integrated with another technique like MS (Theodoridis et al 2012;Khan et al 2017). A recent method for multi-component analysis is the metabolic profiling that is employed for the examination of acids and urinary drugs coupled with GC/MS.…”

Section: Low Molecular Weight Metabolitesmentioning

confidence: 99%

Metabolomics: a systems biology approach for enhancing heat stress tolerance in plants

Raza

2020

Plant Cell Rep

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Key message Comprehensive metabolomic investigations provide a large set of stress-related metabolites and metabolic pathways, advancing crops under heat stress conditions. Metabolomics-assisted breeding, including mQTL and mGWAS boosted our understanding of improving numerous quantitative traits under heat stress. Abstract During the past decade, metabolomics has emerged as a fascinating scientific field that includes documentation, evaluation of metabolites, and chemical methods for cell monitoring programs in numerous plant species. A comprehensive metabolome profiling allowed the investigator to handle the comprehensive data groups of metabolites and the equivalent metabolic pathways in an extraordinary manner. Metabolomics, together with transcriptomics, plays an influential role in discovering connections between stress and genes/metabolite, phenotyping, and biomarkers documentation. Further, it helps to decode several metabolic systems connected with heat stress (HS) tolerance in plants. Heat stress is a critical environmental factor that is globally affecting the growth and productivity of plants. Thus, there is an urgent need to exploit modern breeding and biotechnological tools like metabolomics to develop cultivars with improved HS tolerance. Several studies have reported that amino acids, carbohydrates, nitrogen metabolisms, etc. and metabolites involved in the biosynthesis and catalyzing actions play a game-changing role in HS response and help plants to cope with the HS. The use of metabolomicsassisted breeding (MAB) allows a well-organized transmission of higher yield and HS tolerance at the metabolome level with specific properties. Progressive metabolomics systematic techniques have accelerated metabolic profiling. Nonetheless, continuous developments in bioinformatics, statistical tools, and databases are allowing us to produce ever-progressing, comprehensive insights into the biochemical configuration of plants and by what means this is inclined by genetic and environmental cues. Currently, assimilating metabolomics with post-genomic platforms has allowed a significant division of genetic-phenotypic connotation in several plant species. This review highlights the potential of a state-of-the-art plant metabolomics approach for the improvement of crops under HS. The development of plants with specific properties using integrated omics (metabolomics and transcriptomics) and MAB can provide new directions for future research to enhance HS tolerance in plants to achieve a goal of "zero hunger".

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Chromatographic profiling of Pancharishta at different stages of its development using HPTLC, HPLC, GC–MS and UPLC–MS

Cited by 22 publications

References 24 publications

Hypoglycemic Potential of Aqueous Extract of Moringa oleifera Leaf and In Vivo GC-MS Metabolomics

Hypoglycemic Potential of Aqueous Extract of Moringa oleifera Leaf and In Vivo GC-MS Metabolomics

Analytical Techniques for Metabolomic Studies: A Review

Metabolomics: a systems biology approach for enhancing heat stress tolerance in plants

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