Chromatographic purification and characterization of elicitors of necrosis on tobacco produced by incompatible Phytophthora species

Billard, Valérie; Bruneteau, Maud; Bonnet, Ph.; Ricci, Pierre; Pernollet, J.C.; Huet, Jean‐Claude; Vergne, Amélie L.; Richard, G; Michel, Georges

doi:10.1016/s0021-9673(00)94513-8

Cited by 39 publications

(25 citation statements)

References 11 publications

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“…Not only the amino acid sequences of cinnamomin and cryptogein were more homologous to each other than to capsicein, but they exhibit the same levels of biological activities [1,3,4]. Since the central core of the 3 molecules was highly conserved, it was likely essential for both biological activities.…”

Section: Discussionmentioning

confidence: 99%

“…Cinnamomin was purified as already described [3] and was reduced with dithiothreitol and S-carboxymethylated with unlabeled iodoacetic acid according to .…”

Section: Methodsmentioning

confidence: 99%

“…In contrast, no distant leaf necrosis is observed during the interaction between P. nicotianae and tobacco, and no elicitor of necrosis has been detected in culture filtrates [I]. In culture, P. cryptogea, P. capsici and P. cinnamomi secrete low molecular mass proteins named cryptogein, capsicein and cinnamomin, respectively [2], which have been purified [3]. These fungal proteins constitute a novel protein family, called elicitins.…”

Section: Introductionmentioning

confidence: 99%

“…Cinnamomin, purified from these filtrates [3], shows a level of necrotic activity comparable to cryptogein [1,3] and protects tobacco against its pathogen at similar doses. We have therefore determined the amino acid sequence of cinnamomin to get a more precise knowledge of the amino acid residues which are involved in the modulation of the elicitin biological activities.…”

Section: Introductionmentioning

confidence: 99%

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Amino acid sequence of cinnamomin, a new member of the elicitin family, and its comparison to cryptogein and capsicein

Huet¹,

Pernollet²

1989

FEBS Letters

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The phytopathogenic fungi Phytophthora cinnamom cause systemic leaf necrosis on its non-host tobacco; in culture, it secretes a protein, called cinnamomin, which elicits leaf necrosis and protects tobacco against the pathogen Phytophthora nicotiam, in a way similar to cryptogein and different from capsicem, elicitms of known amino acid sequences. The cinnamomin sequence has been determined and compared to other elicitins. The differences in the 3 elicitin sequences were correlated to the biological acivities: 2 lysines were ascribed as the key amino actds involved in the differential control of protection with respect to necrosis.Amino acrd sequence; Elicitin; Plant pathogen; (Phytophthora, Nicotiana rabacum)

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Section: Discussionmentioning

confidence: 99%

“…Cinnamomin was purified as already described [3] and was reduced with dithiothreitol and S-carboxymethylated with unlabeled iodoacetic acid according to .…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Amino acid sequence of cinnamomin, a new member of the elicitin family, and its comparison to cryptogein and capsicein

Huet¹,

Pernollet²

1989

FEBS Letters

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“…Typical examples of general elicitors are chitin (Felix et al 1993), bacterial flagellin (Felix et al 1999;Zipfel et al 2004), elicitins from Phytophthora species such as cryptogein in their apoplast (Billard et al 1988) or oomycete Pep-13 (Nürnberger et al 1994). Chemical or synthetic compounds also can have elicitor activities in plants.…”

Section: Introductionmentioning

confidence: 99%

Defense induced by a bis-aryl methanone compound leads to resistance in potato against Phytophthora infestans

2013

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Plants recognize certain microbial compounds as elicitors of their active defense mechanisms. In the present study, NUBS-4190, a synthetic bis-aryl-methanone compound elicited NO and ROS generation in potato suspension cultured cells and intact potato leaves. Hypersensitive cell death was found in these cultured cells and in potato leaves without the accumulation of phytoalexins in the tubers. Defense-related genes such as StrbohB, StrbohC, StNR1, StNR5, Sthsr203J and StPR1 were expressed in potato suspension cultured cells treated with NUBS-4190. Resistance against Phytophthora infestans also increased in NUBS-4190-treated potato leaves.

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PopA1, a protein which induces a hypersensitivity-like response on specific Petunia genotypes, is secreted via the Hrp pathway of Pseudomonas solanacearum.

et al. 1994

Self Cite

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This paper describes the identification of a new class of extracellular bacterial proteins, typified by PopA1 and its derivative PopA3, which act as specific hypersensitive response (HR) elicitors. These two heat‐stable proteins, with HR‐like elicitor activities on tobacco (non‐host plant) but without activity on tomato (host plant), have been characterized from the supernatant of the plant pathogenic bacterium Pseudomonas solanacearum strain GMI1000. These two proteins induced the same pattern of response on Petunia, as a function of the genotypes tested. popA, the structural gene for PopA1, maps outside of the hrp gene cluster but belongs to the hrp regulon. The amino acid sequence of PopA1 does not show homology to any characterized proteins. Its secretion is dependent on hrp genes and is followed by stepwise removal of the 93 amino‐terminal amino acids, producing the protein PopA3. Petunia lines responsive to PopA3 and its precursors were resistant to infection by strain GMI1000, whereas non‐responsive lines were sensitive, suggesting that popA could be an avirulence gene. A popA mutant remained fully pathogenic on sensitive plants, indicating that this gene is not essential for pathogenicity. While lacking PopA1, this mutant, which remained avirulent on tobacco and on resistant Petunia lines, still produced additional extracellular necrogenic compounds. On the basis of both their structural features and the biological properties of the popA mutant, PopA1 and PopA3 clearly differ from hairpins characterized in other plant pathogenic bacteria.

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Chromatographic purification and characterization of elicitors of necrosis on tobacco produced by incompatible Phytophthora species

Cited by 39 publications

References 11 publications

Amino acid sequence of cinnamomin, a new member of the elicitin family, and its comparison to cryptogein and capsicein

Amino acid sequence of cinnamomin, a new member of the elicitin family, and its comparison to cryptogein and capsicein

Defense induced by a bis-aryl methanone compound leads to resistance in potato against Phytophthora infestans

PopA1, a protein which induces a hypersensitivity-like response on specific Petunia genotypes, is secreted via the Hrp pathway of Pseudomonas solanacearum.

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