Chromatographic purification of one of the complementary strands of DNA of phage α

Aurisicchio, S.; Dore, E.; Frontali, C.; Gaeta, F.S.; Toschi, G.

doi:10.1016/0926-6550(64)90157-4

Cited by 9 publications

(4 citation statements)

References 8 publications

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“…Preferential binding of poly (G) to the H strand further increases the separation of the two partners. Similar findings were reported for other DNA phages of bacilli (2,15,36,47,48).…”

Section: -3 4~~~2supporting

confidence: 90%

Recombinational-type transfer of viral DNA during bacteriophage 2C replication in Bacillus subtilis

Hoet¹,

Fraselle²,

Cocito³

1976

J Virol

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The Bacillus subtilis phage 2C contains one molecule of double-stranded DNA of about 100 x 101 daltons in which thymine is replaced by hydroxymethyluracil; the two strands have different buoyant densities. Parental DNA, labeled with either [3H]uracil or [32P]phosphate, was quite effectively transferred to offspring phage, and the efficiency of transfer was the same for the two strands. Labeled nucleotide compositions of the H and L strands from parental and progeny virions were very close. These data exclude a degradation of the infecting DNA and reutilization of nucleotides. Upon infection of light unlabeled cells with heavy radioactive viruses, no DNA with either heavy or hybrid density was extracted from offspring phage. Instead, an heterogeneous population of DNA molecules of densities ranging from that of almost hybrid to that of fully light species was obtained. Shear degradation of such progeny DNA to fragments of decreasing molecular weight produced a progressive shift to the density of hybrid molecules. Denaturation of sheared DNA segments caused the appearance of labeled and heavy single-stranded segments. These findings indicate that 2C DNA replicates semiconservatively and then undergoes extensive genetic recombination with newly formed viral DNA molecules within the vegetative pool, thus mimicking a dispersive transfer of the infecting viral genome. The pieces of transferred parental DNA have an average size of 10 x 106 daltons.

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“…Preferential binding of poly (G) to the H strand further increases the separation of the two partners. Similar findings were reported for other DNA phages of bacilli (2,15,36,47,48).…”

Section: -3 4~~~2supporting

confidence: 90%

Recombinational-type transfer of viral DNA during bacteriophage 2C replication in Bacillus subtilis

Hoet¹,

Fraselle²,

Cocito³

1976

J Virol

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“…Finally, three different groups (231)(232)(233)(234) have employed one-and two stranded DNA from bacterial viruses in direct hybridization experiments. The phages used were cfJX 174 (234), where the phage particle yields one stranded DNA and two-stranded phage DNA can be isolated from infected E. coli cells, and the B. subtilis phages IX (23 1, 232) and SP 8 (233) , where the two strands of denatured phage DNA can be separated by density gradient centrifugation.…”

Section: Rna Polymerasementioning

confidence: 99%

Metabolism of Nucleic Acids (Macromolecular DNA and RNA)

Elson¹

1965

Annu. Rev. Biochem.

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Since it is manifestly impossible to cover the entire field of nucleic acid metabolism in one article, I have restricted this review to recent work on enzymes which have to do with macromolecular nucleic acids. Sections are included on the synthetic enzymes DNA polymerase and RNA polymerase and on enzymes which alter the nucleic acids in their macromolecular state, namely, the DNA and RNA methylases. The concluding section deals with degradative enzymes, chiefly RNase, DNase, and polynucleotide phosphoryl ase, but in a much more cursory manner. The review is based on an exten sive, but certainly not complete, coverage of the recent literature. References 1 through 10 are reviews, books, special collections of articles, and critical papers which deal with various aspects of the subject matter of this review. ENZYMES WHICH SYNTHESIZE NUCLEIC ACIDSThe DNA polymerases and RNA polymerases catalyze the synthesis of polynucleotides by the complementary copying of pre-existing polynucleo tides. The precursors are the appropriate nucleoside triphosphates, and a bivalent cation is required (11-16). During the period under review, there have appeared no serious reasons to doubt that these are the principal, and quite possibly the only, enzymes which catalyze the synthesis de novo of DNA and RNA in nature. Beyond this, a great deal remains to be eluci dated, and much of the recent work has dealt with such matters as the chem ical and physical nature of the polynucleotide templates or primers and prod ucts, details of the reaction mechanisms, differences between the reactions in vivo and in vitro, the association of the enzymes with other cell components, and so forth.DNA POLYMERASEThe location of DNA polymerase in the cell.-In bacterial extracts DNA polymerase has been observed to sediment together with DNA (e.g., 17, 18) . In higher organisms, however, a number of studies had indicated that the enzyme is actually located outside the cell nucleus. In view of the unexpected nature of these findings, the matter has been pursued in several laboratories; and reports have now appeared which provide evidence that at least some of

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“…The difficulty of consistently preparing the chromatographic matrix, which consisted of methylated serum albumin adsorbed to kieselguhr, a diatomaceous earth composed principally of silica, also may have been a contributing factor (36). 3 There are significant caveats attached to the latter experiments.…”

Section: Rna Polymerasementioning

confidence: 99%

An Introduction to Transcription and Gene Regulation

Geiduschek

2010

Journal of Biological Chemistry

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Chromatographic purification of one of the complementary strands of DNA of phage α

Cited by 9 publications

References 8 publications

Recombinational-type transfer of viral DNA during bacteriophage 2C replication in Bacillus subtilis

Recombinational-type transfer of viral DNA during bacteriophage 2C replication in Bacillus subtilis

Metabolism of Nucleic Acids (Macromolecular DNA and RNA)

An Introduction to Transcription and Gene Regulation

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