Chromatographic resolution of lysozyme variants

Fausnaugh-Pollitt, Jodi; Thevenon, Gerladine; Janis, Linda J.; Regnier, Fred E.

doi:10.1016/s0021-9673(00)94795-2

Cited by 50 publications

(18 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…As has been shown by many groups, protein shape, size, and charge character strongly affect retention behavior (Fausnaugh-Pollitt et al, 1988;Blanco et al, 1989;R'hitley et al, 1989a). In most cases, aggregation alters the apparent values of these physical properties.…”

Section: Effect Of Aggregation On Parameter Estimationmentioning

confidence: 93%

Effects of protein aggregation in isocratic nonlinear chromatography

et al. 1991

View full text Add to dashboard Cite

Section: Effect Of Aggregation On Parameter Estimationmentioning

confidence: 93%

Effects of protein aggregation in isocratic nonlinear chromatography

et al. 1991

View full text Add to dashboard Cite

“…Worth mentioning is that the retention properties of large peptides in both HILIC and RP-HPLC can be understood by the contact region concept [63]. Using mixed-…”

Section: Mechanismmentioning

confidence: 99%

Hydrophilic interaction chromatography

Hemström

Irgum²

2006

J of Separation Science

1,087

818

View full text Add to dashboard Cite

Separation of polar compounds on polar stationary phases with partly aqueous eluents is by no means a new separation mode in LC. The first HPLC applications were published more than 30 years ago, and were for a long time mostly confined to carbohydrate analysis. In the early 1990s new phases started to emerge, and the practice was given a name, hydrophilic interaction chromatography (HILIC). Although the use of this separation mode has been relatively limited, we have seen a sudden increase in popularity over the last few years, promoted by the need to analyze polar compounds in increasingly complex mixtures. Another reason for the increase in popularity is the widespread use of MS coupled to LC. The partly aqueous eluents high in ACN with a limited need of adding salt is almost ideal for ESI. The applications now encompass most categories of polar compounds, charged as well as uncharged, although HILIC is particularly well suited for solutes lacking charge where coulombic interactions cannot be used to mediate retention. The review attempts to summarize the ongoing discussion on the separation mechanism and gives an overview of the stationary phases used and the applications addressed with this separation mode in LC.

show abstract

“…Is there really an area on the protein with z,, charges that is Fausnaugh-Pollitt et al (1988) 2.1-3.1 Kopaciewicz et al (1983) 3.6-8.3 Raje and Pinto (1997) 4.8-6.0 -0.2-3.6 responsible for the binding to the chromatographic contact area? Is there only one area or are there more'?…”

Section: Binding Chargementioning

confidence: 99%

“…Fausnaugh-Pollitt et al (1988) were able to separate three variants of lysozyme varying by a single charged amino acid group on a cation exchanger. They concluded that although charged groups at different locations in a molecule are sensed by the sorbent, their contributions may not be equivalent.…”

Section: Aiche Journalmentioning

confidence: 99%