Chromatographic separation and analysis of chloropheniramine maleate, methscopolamine nitrate and phenylephrine hydrochloride in sustained release capsules

Abstract

SummaryA high performance liquid chromatographic method has been developed for the simultaneous determination of chlorpheniramine maleate (CPM), methscoplamine nitrate (MSN) and phenylephrine hydrochloride (PEH) in sustained release capsules. The separation was carried out on a reverse-phase CN-column with use of a mobile phase consisting of 70 % (v/v) solution of acetonitrile in water containing 2 % (v/v) acetic acid and 0.005M sodium 1-heptane sulfonate at a flow rate of 2 mL min 1. The eluted peaks were detected at 262 nm. The method is sensitive, accurate and rapid and can be used in the routine analysis of the mixture of the three compounds.

Preparation of New Molecularly Imprinted Polymers and its Use in the Selective Extraction For Determination Phenylephrine Hydrochloride at Pharmaceuticals

“…According to review, phenylephrine hydrochloride was determination by several methods such as these recent spectrophotometry, 5 spectrophotometry method for estimating infinitesimal amounts of phenylephrine hydrochloride, 6 HPLC reversed-phase technique, 7 HPLC technique, 8 liquid chromatography technique, 9 thin layer chromatography technique, 10 voltammetric differential Pulse Method, 11 capillary electrophoresis method, 12 combining liquid chromatography and mass spectrometry techniques and comparing results with HPLC-UV, 13 micellar electrokinetic chromatography *Author for Correspondence: yahyaalbayti@yahoo.com method. 14 Several techniques used for determination drugs and pharmaceuticals.…”

Analysis of pharmaceutical preparations containing antihistamine drugs by micellar liquid chromatography

“…The determination of antihistamines in pharmaceutical preparations for its quality control has been performed using several analytical techniques, such as volumetric analysis [2,3], voltametria [4], atomic absorption [5], fluorimetry [6,7], spectrophotometry [8][9][10][11][12], gas chromatography [13] liquid chromatography [14][15][16][17], capillary electrophoresis [18] and micellar electrokinetic chromatography [19,20].…”

Determination of prednisolone, naphazoline, and phenylephrine in local pharmaceutical preparations by micellar electrokinetic chromatography

“…The methods described for the assay of PHE are UV spectrophotometry for amphetamines [9], HPLC for catecholamines [10], and capillary zone electrophoresis for other beta-amino alcohols [11] and amphetamine [12] using a phosphate buffer (pH 3.2) and beta-cyclodextrin to obtain an enantiomeric separation.…”

“…Prednisolone (PRE) is determined in combination with other natural and synthetic corticosteroids [2,3] in pharmaceuticals with a limit of detection (LOD) around 1 mg/L and together with their metabolites [4] in biological fluids by reversed phase HPLC, by liquid chromatography-ion spray mass spectrometry [5,6], and by MEKC in serum after prior SPE [7,8] using phosphate-borate buffer (pH 8) with SDS and 16% acetonitrile with detection at 254 nm in 10 min with limits of quantification (LOQ) of 0.5 mg/L. The methods described for the assay of PHE are UV spectrophotometry for amphetamines [9], HPLC for catecholamines [10], and capillary zone electrophoresis for other beta-amino alcohols [11] and amphetamine [12] using a phosphate buffer (pH 3.2) and beta-cyclodextrin to obtain an enantiomeric separation.…”