Chromatographic separation and determination of fruit acids

Goudie, Alexander J.; Rieman, William

doi:10.1016/s0003-2670(00)88408-1

Cited by 11 publications

(3 citation statements)

References 9 publications

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“…Fruits, which contain a mixture of cis-diols such as 1,2-diphenols, alditols, α-hydroxy acids and saccharides [39][40][41] , were used as an example for this demonstration. These cis-diols react reversibly with phenylboronic acid (PBA) in an aqueous environment.…”

Section: Nanopore Signatures Of Model Cis-diolsmentioning

confidence: 99%

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Nanopore analysis of cis-diols in fruits

Fan,

Cao,

Zhang

et al. 2024

Nat Commun

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Natural fruits contain a large variety of cis-diols. However, due to the lack of a high-resolution sensor that can simultaneously identify all cis-diols without a need of complex sample pretreatment, direct and rapid analysis of fruits in a hand-held device has never been previously reported. Nanopore, a versatile single molecule sensor, can be specially engineered to perform this task. A hetero-octameric Mycobacterium smegmatis porin A (MspA) nanopore modified with a sole phenylboronic acid (PBA) adapter is prepared. This engineered MspA accurately recognizes 1,2-diphenols, alditols, α-hydroxy acids and saccharides in prune, grape, lemon, different varieties of kiwifruits and commercial juice products. Assisted with a custom machine learning program, an accuracy of 99.3% is reported and the sample pretreatment is significantly simplified. Enantiomers such as DL-malic acids can also be directly identified, enabling sensing of synthetic food additives. Though demonstrated with fruits, these results suggest wide applications of nanopore in food and drug administration uses.

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Section: Nanopore Signatures Of Model Cis-diolsmentioning

confidence: 99%

“…2a). They represent 1,2-diphenols, alditols, αhydroxy acids and saccharides that are widely encountered in fruits [39][40][41] . MspA-PBA, a hetero-octameric MspA nanopore modified with a sole phenylboronic acid (PBA) reactive adapter at site 90 Fig.…”

Section: Nanopore Signatures Of Model Cis-diolsmentioning

confidence: 99%