Chromatographic Separation of Antihistamine Drugs using HPLC

Abstract

The chromatographic separation of antihistamine drugs using High-Performance Liquid Chromatography (HPLC) has garnered significant attention due to its pivotal role in pharmaceutical analysis. Antihistamines, vital in managing allergic disorders, present complex challenges in terms of analytical assessment owing to their diverse chemical structures and stereochemistry. This review aims to provide an overview of one of the best methodologies employed in HPLC-based separation of antihistamine drugs. Emphasis is placed on the separation of both nonracemic and racemic forms, considering their distinct pharmacological profiles and therapeutic implications. Various aspects, including method development, optimization strategies, column selection, and detection techniques, are scrutinized to highlight the crucial parameters influencing separation efficiency and accuracy. Furthermore, the review delves into the application of chiral HPLC methods for resolving enantiomeric mixtures of antihistamines, underscoring their importance in pharmaceutical research and development. By synthesizing recent literature findings and methodological advancements, this review offers insights into the current state-of-the-art in HPLC-based separation of antihistamine drugs and outlines future directions for research in this critical field.